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| Development of a Multiplex PCR for Diagnosis of Staphylococcus aureus, Escherichia coli and Bacillus cereus from Cows with Endometritis |
| SUNDong-bo, WURui, HEXian-jing, WANGShuang, LINYun-cheng, HANXu, WANGYue-qiang, GUOTing-ting, WUGuo-jun , YANGKe-li |
| 1.College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University |
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摘要 Staphylococcus aureus, Escherichia coli and Bacillus cereus are the major agents of cow endometritis in dairy cows. A multiplex PCR (SEB-mPCR) was established based on the conserved genes of S. aureus, E. coli and B. cereus, and the detection limits were 103, 102 and 103 CFU mL-1, respectively. SEB-mPCR could not amplify genomic DNA of pathogenic bacteria of other common bovine diseases. A total of 309 vaginal discharge samples from cows with endometritis were tested by SEB-mPCR. Of the samples, 23.95% had the three kinds of bacteria detected, 17.15% had S. aureu and E. coli, 9.39% had E. coli and B. cereus, and 9.71% had S. aureus and B. cereus. The rates of infections with S. aureus, E. coli and B. cereus were 11.35, 16.18 and 9.06%, respectively. Therefore, SEB-mPCR has a potential as a diagnosis tool for endometritis in dairy cows.
Abstract Staphylococcus aureus, Escherichia coli and Bacillus cereus are the major agents of cow endometritis in dairy cows. A multiplex PCR (SEB-mPCR) was established based on the conserved genes of S. aureus, E. coli and B. cereus, and the detection limits were 103, 102 and 103 CFU mL-1, respectively. SEB-mPCR could not amplify genomic DNA of pathogenic bacteria of other common bovine diseases. A total of 309 vaginal discharge samples from cows with endometritis were tested by SEB-mPCR. Of the samples, 23.95% had the three kinds of bacteria detected, 17.15% had S. aureu and E. coli, 9.39% had E. coli and B. cereus, and 9.71% had S. aureus and B. cereus. The rates of infections with S. aureus, E. coli and B. cereus were 11.35, 16.18 and 9.06%, respectively. Therefore, SEB-mPCR has a potential as a diagnosis tool for endometritis in dairy cows.
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Received: 24 August 2010
Accepted:
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| Fund: This work was supported by a grant from the Startup Foundation for Doctors of Heilongjiang Bayi Agricultural University, China (B2009-4). |
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Corresponding Authors:
Correspondence WU Rui, Professor, Tel: +86-459-6819191, Fax: +86-459-6819191, E-mail: fuhewu@126.com
E-mail: fuhewu@126.com
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| About author: SUN Dong-bo, Ph D, E-mail: dongbosun@yahoo.com.cn |
Cite this article:
SUNDong-bo , WURui , HEXian-jing , WANGShuang , LINYun-cheng , HANXu , WANGYue-qiang , GUOTing-ting , WUGuo-jun , YANGKe-li .
2011.
Development of a Multiplex PCR for Diagnosis of Staphylococcus aureus, Escherichia coli and Bacillus cereus from Cows with Endometritis. Journal of Integrative Agriculture, 10(10): 1624-1629.
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