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| Anti-Recombinant Gametocyte 56 Protein IgY Protected Chickens from Homologous Coccidian Infection |
| DING Jun, LIU Qiao-rong, HAN Jin-peng, QIAN Wei-feng, LIU Qun |
| 1.Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture/College of Veterinary Medicine, China Agricultural University, Beijing 100193, P.R.China |
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摘要 Coccidiosis is caused by intra-cellular infection of Eimeria spp., which goes through a complex life cycle in the intestinal mucosa of infected hosts. Specific immunoglobulins (IgY) could be produced in egg yolk by immunizing hens with specific antigens. In the present study, we cloned the E. maxima gam56 gene, expressed the GST-GAM56 fusion protein and raised IgY to GST-GAM56 in hens. The anti-GST-GAM56 IgY antibody was isolated and used to treat chickens infected with E. maxima oocysts. Intramuscular injection of the antibodies provided minimal protection against parasite infection. However, oral dosing of the IgY 3 or 5 d after oocyst inoculation significantly improved body weight gain, reduced oocyst output and intestinal lesion score were reduced at 3 or 5 d after oocyst challenging, compared to the untreated control group. Our findings suggest that the IgY to gam56 could be an effective prophylactic or therapeutic agent against E. maxima infection in chickens and should have a practical application value.
Abstract Coccidiosis is caused by intra-cellular infection of Eimeria spp., which goes through a complex life cycle in the intestinal mucosa of infected hosts. Specific immunoglobulins (IgY) could be produced in egg yolk by immunizing hens with specific antigens. In the present study, we cloned the E. maxima gam56 gene, expressed the GST-GAM56 fusion protein and raised IgY to GST-GAM56 in hens. The anti-GST-GAM56 IgY antibody was isolated and used to treat chickens infected with E. maxima oocysts. Intramuscular injection of the antibodies provided minimal protection against parasite infection. However, oral dosing of the IgY 3 or 5 d after oocyst inoculation significantly improved body weight gain, reduced oocyst output and intestinal lesion score were reduced at 3 or 5 d after oocyst challenging, compared to the untreated control group. Our findings suggest that the IgY to gam56 could be an effective prophylactic or therapeutic agent against E. maxima infection in chickens and should have a practical application value.
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Received: 13 June 2011
Accepted:
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| Fund: This work was supported by the Program for Cheung Kong Scholars and Innovative Research Team in University of China (IRT0866). |
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Corresponding Authors:
Correspondence LIU Qun, Tel: +86-10-62734496, E-mail: qunliu@cau.edu.cn
E-mail: qunliu@cau.edu.cn
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| About author: DING Jun, E-mail: dingjun407@163.com |
Cite this article:
DING Jun, LIU Qiao-rong, HAN Jin-peng, QIAN Wei-feng, LIU Qun.
2012.
Anti-Recombinant Gametocyte 56 Protein IgY Protected Chickens from Homologous Coccidian Infection. Journal of Integrative Agriculture, 12(10): 1721-1728.
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