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| In vitro establishment of a highly effective method of castor bean (Ricinus communis L.) regeneration using shoot explants |
| ZHANG Ji-xing1, 2, WANG Xiao-yu3, FENG Zi-zhou1, 4, GENG Xue-jun1, MU Sha-moli1, 2, HUO Hong-yan1, TONG Huan5, LI Meng-zhu6, LI Yi6, CHI Yue1, 2, CHEN Yong-sheng1, 2 |
1 School of Life Science, Inner Mongolia University for Nationalities, Tongliao 028000, P.R.China
2 Inner Mongolia Industrial Engineering Research Center of Universities for Castor, Tongliao 028000, P.R.China
3 College of Plant Science, Jilin University, Changchun 130062, P.R.China
4 College of Agriculture, Inner Mongolia University for Nationalities, Tongliao 028000, P.R.China
5 School of Life Science, Nanjing Agricultural University, Nanjing 210095, P.R.China
6 School of Life Science, Shandong University, Jinan 25000, P.R.China |
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Abstract An efficient plant regeneration protocol was established for castor bean (Ricinus communis L.), in which 0.3 mg L–1 thidiazuron (TDZ) induced shoot clusters and increased the number of adventitious shoots from hypocotyl tissue. Our results showed that treatment under dark conditions significantly promoted the average number of shoots per explant to 37.36±4.54 (with a 6-d treatment). Modified 1/2 Murashige and Skoog (MS) basal medium supplemented with 440 mg L–1 Ca2+, 0.2 mg L–1 gibberellic acid and 0.1 mg L–1 TDZ significantly increased shoot elongation rates and lowered vitrification rates. Furthermore, 1/2 MS media supplemented with 0.2 mg L–1 1-naphthaleneacetic acid induced a higher rooting rate compared with other culture conditions.
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Received: 28 October 2015
Accepted:
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| Fund: This research was supported by the National Natural Science Foundation of China (31260336 and 31460353). |
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Corresponding Authors:
CHEN Yong-sheng,
E-mail: chenyongshengnmg@hotmail.com
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| About author: ZHANG Ji-xing, Tel: +86-475-8314509, E-mail: 15924475588 @163.com |
Cite this article:
ZHANG Ji-xing, WANG Xiao-yu, FENG Zi-zhou, GENG Xue-jun, MU Sha-moli, HUO Hong-yan, TONG Huan, LI Meng-zhu, LI Yi, CHI Yue, CHEN Yong-sheng.
2016.
In vitro establishment of a highly effective method of castor bean (Ricinus communis L.) regeneration using shoot explants. Journal of Integrative Agriculture, 15(06): 1417-1422.
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