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1. 基于改进Cascade R-CNN算法的黄粘板图像上十字花科蔬菜害虫田间智能监测系统
Yufan Gao, Fei Yin, Chen Hong, Xiangfu Chen, Hang Deng, Yongjian Liu, Zhenyu Li, Qing Yao
Journal of Integrative Agriculture    2025, 24 (1): 220-234.   DOI: 10.1016/j.jia.2024.06.017
摘要83)      PDF    收藏

十字花科蔬菜是重要的食用蔬菜,但在生长过程中极易受到害虫的影响。为了实现虫情测报和科学防治,需要对这些害虫进行实时准确的监测。目前,悬挂黄粘板是黄色趋向性蔬菜害虫监测和诱捕的常用方法。为了实现对粘虫板上蔬菜害虫的实时、低成本、智能化监测,我们建立了一个由智能摄像头、Web平台和部署在服务器上的害虫检测算法组成的智能监测系统。工作人员在系统平台上设置好摄像头的拍摄预置点和拍摄时间后,布置在田间的摄像头每天定点、定时自动采集多张黄粘板的图像。黄粘板上诱捕的害虫包括小菜蛾(Plutella xylostella)、黄曲条跳甲(Phyllotreta striolata)、蝇类(Muscidae)三种,它们的虫体小且易破损,增加了模型检测的难度。针对害虫体型小、易破损导致的识别效果差的问题,我们提出了基于改进Cascade R-CNN模型的蔬菜害虫智能检测算法来识别这三种目标害虫。该算法引入了重叠滑动窗口方法,使用改进的Res2Net网络作为骨干网络,同时采用递归特征金字塔网络作为特征融合网络。测试结果表明,该算法对黄粘板图像上的三种目标害虫取得了良好的检测效果,精确率分别为96.5%92.2%75.0%,召回率分别为96.6%93.1%74.7%F10.880。与其他算法相比,我们的算法在检测小目标害虫的能力上具有明显优势。为了准确获取图像上每天新增的害虫,我们提出了一种基于双阶段匹配的蔬菜害虫匹配计数算法,测试结果显示,该算法取得了与手工统计结果高度一致的害虫增长情况,平均误差仅2.2%。蔬菜害虫智能监测系统实现了蔬菜害虫监测的精准化、可视化和智能化,为农户防控害虫提供依据,具有重要意义。

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2. 基于SSR分子标记的安徽省茶树遗传多样性分析及核心种质构建
TAO Ling-ling, TING Yu-jie, CHEN Hong-rong, WEN Hui-lin, XIE Hui, LUO Ling-yao, HUANG Ke-lin, ZHU Jun-yan, LIU Sheng-rui, WEI Chao-ling
Journal of Integrative Agriculture    2023, 22 (9): 2719-2728.   DOI: 10.1016/j.jia.2023.07.020
摘要255)      PDF    收藏

茶树是中国主要经济作物之一,安徽省茶树栽培历史悠久具有丰富的种质资源和遗传多样性茶树的高度杂合性导致其种质资源收集、管理及保护等方面工作进展缓慢,并且长期保存管理成本高,因此提高安徽省茶树种质资源的管理质量和效率,构建核心种质迫在眉睫本研究从安徽省6个主要产茶区收集了573份有代表性的茶树基于60SSR分子标记进行系统发育关系、群体结构和主坐标分析。聚类结果显示安徽省573茶树被分5个类群,这些类群分布地区收集样品的地理位置相关,聚类结果PCoA分群结果也基本一致。最后,我们构建了一个由115茶树品种组成的核心种质,占原始种质的20%。核心种质观察到的等位基因数(Na)具有90.9%的保留率,并且核心种质和原始种质的香农信息指数(I)及其他遗传多样性参数之间没有显著性差异所有代表性茶区均保留了部分代表性品种,其中黄山地区保留了39份茶树,占核心种质的33.9%金寨县保留了10份茶树,占核心种质的8.9%PCoA结果表明构建的核心种质均匀的分布在收集的安徽省茶树种质中,说明构建的核心种质能够代表安徽省茶树种质的遗传多样性。本研究对安徽省茶树种质资源的高效保存和利用具有一定的参考价值。

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3. JIA-2021-1309 一个RNaseH-like蛋白LHL1通过影响生长素信号调控水稻毛状体形成
CHEN Hong-yan, ZHU Zhu, WANG Xiao-wen, LI Yang-yang, HU Dan-ling, ZHANG Xue-fei, JIA Lu-qi, CUI Zhi-bo, SANG Xian-chun
Journal of Integrative Agriculture    2023, 22 (1): 31-40.   DOI: 10.1016/j.jia.2022.08.101
摘要494)      PDF    收藏
水稻叶片表皮毛是由表皮细胞分化发育形成,表皮毛在植物的抗逆以及防止紫外直射等过程中都具有重要的作用,但关于水稻毛状体发育的研究还存在很大未知。在本研究中,我们对野生型籼系水稻西大1B进行EMS (ethylmethane sulfonate) 诱导,通过表型观察分析筛选出了毛状体发育缺陷突变体,将其命名为lhl1 (Less Hairy Leaf 1)。我们对其进行了基因定位和图位克隆,将其定位在2号染色体两个分子标记的70 kb的区间内,通过基因测序将LOC_Os02g25230确定为候选基因。之后我们构建了干涉以及超表达株系,扫描电镜观察分析发现LHL1-RNAi叶片同lhl1一样存在毛状体发育缺陷,但LHL1-OE株系叶片表面的毛状体形态与野生型相似,但数目大大增加。qRT-PCR分析发现,与野生型相比,突变体lhl1中正向调控毛状体发育相关的基因表达下调。对生长素相关基因定量分析发现,突变体hl7中生长素相关基因的表达严重下调,进一步通过激素响应分析发现HL7的表达受到生长素的诱导,证明了HL7影响水稻叶片毛状体的发育可能与生长素途径有关。
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4. JIA-2021-0331 高日产机插中籼杂交稻生长及产量特征研究
DENG Fei, HE Lian-hua, CHEN Duo, ZHANG Chi, TIAN Qing-lan, WU Zhen-yuan, LI Qiu-ping, ZENG Yu-ling, ZHONG Xiao-yuan, CHEN Hong, WANG Li, REN Wan-jun
Journal of Integrative Agriculture    2022, 21 (9): 2547-2558.   DOI: 10.1016/j.jia.2022.07.030
摘要200)      PDF    收藏

该研究以西南地区近年来审定的20个杂交籼稻品种为主要研究对象,以日产量为聚类指标将20个水稻品种分类为高日产、中日产和低日产3个类型,并进一步分析不同日产量类型水稻生育进程、分蘖成穗、干物质积累转运及产量构成的差异。较低日产类型水稻,高日产类型水稻生育期缩短5-6d,分蘖发生速率则提高了13.33-16.13%。从干物质积累转运特性来看,高日产类型水稻拔节前干物质积累速率、积累量和比例显著低于中日产类型水稻,但其拔节-齐穗和齐穗-成熟阶段干物质积累速率均明显高于中日产和低日产类型水稻,从而提高了成熟期生物量。尽管,高日产类型水稻千粒重不及中日产和低日产类型水稻,但其每穗颖花数显著提高,加之结实率高,齐穗后穗部干物质积累量和营养器官干物质转运量大,最终使其产量较中日产和低日产类型显著提高了6.51–23.16%。综合来看,高日产类型机插杂交籼稻具有分蘖发生快,每穗颖花数高,拔节后生长速率快,齐穗期茎鞘干物质分配比例高的特征


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5. JIA-2021-0994 苹果茎沟病毒与黄金蜜柚黄化斑驳花叶病相关性研究
XUAN Zhi-you, ZHANG Song, LI Ping, YANG Fang-yun, CHEN Hong-ming, LIU Ke-hong, ZHOU Yan, LI Zhong-an, ZHOU Chang-yong, CAO Meng-ji
Journal of Integrative Agriculture    2022, 21 (7): 2031-2041.   DOI: 10.1016/S2095-3119(21)63823-6
摘要201)      PDF    收藏

苹果茎沟病毒 (apple stem grooving virus, ASGV) 是一种重要的潜隐类果树病毒,对柑橘、梨和苹果等多种果树的生产构成了严重的威胁。2018年,在中国南方广泛种植的黄金蜜柚 (Citrus grandis cv. Huangjinmiyou) 上观察到了严重的黄化、斑驳和花叶症状,推测其可能由病毒引起。取5株表现相关症状果树的叶片样品构建混库并送高通量测序分析,从其中鉴定到了3个ASGV变异体,通过RT-PCR和RACE技术验证了其基因组序列。序列分析显示,这3个变异体的基因组核苷酸序列一致性为81.03%–82.34%,其基因组结构与过往报道的侵染其它果树的变异体类似。基于病毒全基因组核苷酸序列和外壳蛋白氨基酸序列的系统发育分析显示,3个黄金蜜柚ASGV变异体分别与来自不同寄主和地区的ASGV变异体聚在一枝。重组分析显示,3个ASGV变异体可能来自于ASGV不同株系间的重组。在全国11个主要柑橘种植省份采集了507份黄金蜜柚样品进行RT-PCR检测发现,在每个省份表现上述相关症状的样品中,ASGV的检出率均在92.7%以上,而在40份没有症状的样品中,均未检测到ASGV。将其中6个省份的感病样品嫁接到ASGV的指示植物——Rusk枳橙上,新生的系统叶表现出典型的碎叶症状,进一步验证了黄金蜜柚中ASGV的侵染。进一步探究了病毒和症状与温度的关系,发现嫁接的感病黄金蜜柚样品在30°C–35°C条件下症状消失,同时RT-PCR也检测不到ASGV的存在。而后,再将其置于20°C–24°C的条件下一段时间后,黄金蜜柚症状恢复,且ASGV可以由RT-PCR检测到。本文揭示了黄金蜜柚黄化斑驳花叶病与ASGV侵染的相关性,并提示了该病害大面积流行的风险,为进一步的病害防控提供了相应参考


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6. JIA-2021-0997 HBP1通过直接增强JAK2的表达来激活STAT3信号通路从而抑制鸡前脂肪细胞分化
CHEN Hong-yan, CHENG Bo-han, MA Yan-yan, ZHANG Qi, LENG Li, WANG Shou-zhi, LI Hui
Journal of Integrative Agriculture    2022, 21 (6): 1740-1754.   DOI: 10.1016/S2095-3119(21)63895-9
摘要320)      PDF    收藏

本研究以东北农业大学肉鸡高、低腹脂双向选择品系1-7周龄鸡只和永生化鸡前脂肪细胞(ICP2细胞)为材料,利用RT-qPCR和Western blot方法分析HBP1在脂肪组织和前脂肪细胞分化过程中的表达模式;以稳定过表达HBP1的ICP2细胞、敲除HBP1的ICP2细胞以及各自的对照细胞为材料,利用油红O染色、RT-qPCR和Western blot检测过表达/敲除HBP1对鸡前脂肪细胞分化的影响;利用信号通路分析试剂盒筛选HBP1调控鸡前脂肪细胞分化的潜在信号通路;在稳定过表达HBP1的ICP2细胞中添加信号转导和转录激活因子3(STAT3)的化学抑制剂或转染siRNA进行功能拯救实验。结果:基因表达分析结果表明,HBP1的表达与鸡腹部脂肪沉积和前脂肪细胞分化有关。过表达HBP1抑制鸡前脂肪细胞分化(P<0.05),敲除HBP1促进鸡前脂肪细胞分化(P<0.05)。进一步研究发现,HBP1靶向激活Janus激酶2(JAK2)基因的转录来激活STAT3信号通路。功能拯救实验结果表明,STAT3信号介导了HBP1对鸡前脂肪细胞分化的调控作用。以上结果表明,HBP1通过直接上调JAK2的表达来激活STAT3信号通路,从而抑制鸡前脂肪细胞的分化。本研究阐明了HBP1在鸡前脂肪细胞分化中的基本功能,并揭示了其部分分子机制。这些发现为进一步解析鸡脂肪组织生长发育的分子遗传基础提供了新的见解。


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7. 华东南地区机械化栽培下头季稻氮肥的优化管理及其对再生稻的递延效应
HUANG Jin-wen, WU Jia-yi, CHEN Hong-fei, ZHANG Zhi-xing, FANG Chang-xun, SHAO Cai-hong, LIN Wei-wei, WENG Pei-ying, Muhammad Umar KHAN, LIN Wen-xiong
Journal of Integrative Agriculture    2022, 21 (2): 351-364.   DOI: 10.1016/S2095-3119(21)63668-7
摘要255)      PDF    收藏

本试验以甬优1540”为材料,通过2年的田间重复试验,在季稻总施纯氮量(225.00kg·hm-2)不变的前提下,设置季不同氮肥运筹处理(基肥:第一次分蘖肥:第二次分蘖肥:穗肥比分别为3:1:2:4(N1)3:2:1:4(N2)3:3:0:4(N3)4:3:0:3(N4)),从水稻群体分蘖特性、冠层结构特点、光合生理变化及干物质积累与运转规律入手,探讨了东南稻作区机械化栽培下季稻氮肥运筹对再生稻产量形成的影响。结果表明,季前氮后移施肥的N1处理有助于增加季分蘖数,提高分蘖成穗率,N1处理成穗率高达70.18%,比基蘖肥比重高的当地常规施肥N4处理提高了9.15%N1处理还有助于提高群体LAI值,其季及再生季齐穗期LAI值分别比N4处理提高了16.52% 29.87%。就茎鞘物质运转率及冠层光能截获率而言,N1处理下其季稻茎鞘物质运转率比N4处理提高了50.57%,而其再生季齐穗期的冠层光能截获率比N4处理提高了5.07%在本试验中,头季稻前氮后移的N1处理两季实际总产量最高,2年平均实际总产达17351.23 kg·hm-2,比当地常规施肥N4处理提高了23.00%。由此可见,季稻合理氮肥处理有利其提高健桩存量,从而为再生季作物的群体及产量形成奠定基础。在本研究中,低留桩机械化栽培下季稻适当的前氮后移,不仅提高季稻产量,而且还有利其再生腋芽的萌发,增加再生季成穗率,从而实现水稻一种两收目的。

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8. Construction of a high-density adzuki bean genetic map and evaluation of its utility based on a QTL analysis of seed size
WANG Li-xia, WANG Jie, LUO Gao-ling, YUAN Xing-xing, GONG Dan, HU Liang-liang, WANG Su-hua, CHEN Hong-lin, CHEN Xin, CHENG Xu-zhen
Journal of Integrative Agriculture    2021, 20 (7): 1753-1761.   DOI: 10.1016/S2095-3119(20)63343-3
摘要162)      PDF    收藏

小豆(Vigna angularis (Willd.) Ohwi & Ohashi) 属于豇豆属亚洲豇豆亚属,是东亚各国传统种植作物。小豆营养丰富、医食两用,消费市场逐渐遍布全球。然而,小豆的遗传研究相对缓慢,导致育种技术落后、效率低下,难以满足生产和市场的需求。本研究基于高通量基因组测序技术构建了小豆SNP高密度遗传连锁图谱,该图谱共11条连锁群,含2904个标记,每条连锁群的标记数从208个(LG7)到397(LG1)个不等。图谱总长1365.0cM,标记间平均距离0.47cM,每条连锁群的长度从97.4cM(LG9)到155.6cM(LG1)不等。利用该图谱共发掘到两个与籽粒大小有关的主效QTL,分别位于LG2(22.1%) 和LG 9(18.8%)。此外,基于InDel侧翼序列,进一步开发了9718对引物,并随机选择200对进行PCR扩增检验,结果显示有75对在24份小豆种质中具有多态性。本研究中高密度图谱构建及籽粒大小的QTL 分析将进一步提升小豆重要性状基因的发掘等,而InDel标记的开发将有效促进小豆种质资源的遗传多样性分析、基因初步定位等研究。


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9. The impact of the New Rural Cooperative Medical Scheme on the “health poverty alleviation” of rural households in China
QIN Li-jian, Chien-ping CHEN, LI Yu-heng, SUN Yan-ming, CHEN Hong
Journal of Integrative Agriculture    2021, 20 (4): 1068-1079.   DOI: 10.1016/S2095-3119(20)63372-X
摘要138)      PDF    收藏

基于全国性大规模的2016年度中国家庭追踪调查数据,使用工具变量法的IVProbit模型,本文实证研究了新型农村合作医疗保险对农村居民家庭健康脱贫的影响。研究发现,第一,新型农村合作医疗保险对农村居民家庭健康脱贫的效果显著。家庭成员患病进行住院治疗,增加了该家庭陷入贫困的风险,新型农村合作医疗保险显著降低了中国农村居民家庭陷入贫困的概率。第二,新农合对农村家庭健康脱贫的影响在不同收入人群之间存在显著的差别。新农合对中高收入组和高收入组农村家庭的健康脱贫没有影响,但是显著提高了低收入组、尤其中低收入组的农村家庭,其因病致贫和因病返贫的防范能力。第三,新农合对农村家庭健康脱贫的影响效果存在显著的地区差异。新农合显著降低了西部地区农村居民家庭陷入贫困的风险,对东部和中部地区的农村居民家庭没有影响。为了减少和消除贫困,增强农村居民的收入获取能力,本文建议采取以下措施:切实提高新型农村合作医疗保险的实际补偿比、积极推进新农合的支付方式改革以控制医疗费用增长、加强西部地区的医疗卫生服务综合体系建设、加强边缘贫困人口医疗保障的制度建设,以及加强农村地区的人居健康环境改造。


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10. Herbicidal activity of Aureobasidium pullulans PA-2 on weeds and optimization of its solid-state fermentation conditions
GUO Qing-yun, CHENG Liang, ZHU Hai-xia, LI Wei, WEI You-hai, CHEN Hong-yu, GUO Liang-zhi, WENG Hua, WANG Jian
Journal of Integrative Agriculture    2020, 19 (1): 173-182.   DOI: 10.1016/S2095-3119(19)62738-3
摘要127)      PDF    收藏
Fungal strain PA-2 was isolated from infected poplar leaves from the Ping’an District, Haidong City of Qinghai Province, China.  Based on the culture characteristics and the internal transcribed spacer (ITS) sequence of its 16S rDNA, the strain was identified as Aureobasidium pullulans.  The culture and metabolites of strain PA-2 showed high herbicidal potential to five tested weeds Galium aparine var. tenerum, Chenopodium album, Malva crispa, Polygonum lapathifolium and Avena fatua.  For the in vitro test, 5 days after the detached leaves were inoculated with PA-2 culture, all leaves infected by the hyphae and became black and rotten.  For the in vivo test, a metabolite filtrate of PA-2 culture was sprayed over the living weed plants, and five days after inoculation, the weed plants became withered and necrotic.  Seven days after inoculation, the fresh weight reductions of G. aparine var. tenerum, C. album, M. crispa, P.?lapathifolium and A.?fatua were 87.25, 78.46, 82.25, 62.11, and 80.27%, respectively.  Galium aparine var. tenerum and M. crispa exhibited significant reductions in fresh weight.  The bio-safety test on the five crops showed no significant plant height reductions, which was also observed for wheat (Triticum aestivum), faba bean (Vicia faba), and barley (Hordeum vulgare).  By contrast, oilseed rape (Brassica napus) and pea (Pisum sativum) exhibited light spots but no significant reductions in plant height.  These results indicated that A.?pullulans could be a potential microbial herbicide for the control of the target weeds in crops.  Optimization of the carbon and nitrogen sources for cultural media and substances for solid-state fermentation indicated that PA-2 had better colony growth and spore production with the optimal carbon source of glucose (C4), nitrogen source of soybean flour (N2), and the optimal substance was wheat bran.  The results in this study provide useful information for the development of A.?pulluans PA-2 as an herbicide for bio-control of the weed.
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11. Insights into African swine fever virus immunoevasion strategies
WANG Jun, SHI xin-jin, SUN Hai-wei, CHEN Hong-jun
Journal of Integrative Agriculture    2020, 19 (1): 11-22.   DOI: 10.1016/S2095-3119(19)62762-0
摘要107)      PDF    收藏
African swine fever (ASF) is an acute and highly contagious disease that causes severe economic losses to the swine industry.  ASF is caused by infection of African swine fever virus (ASFV) in domestic pigs, leading to almost 100% mortality.  However, no effective vaccines and pharmacologic treatment against ASF are available.  ASF poses a severe threat to the swine industry and the economy.  Here we summarize potential virus-host cell interaction mechanisms involving the suppression of innate and adaptive immune responses to ASFV entry and infection.  These mechanisms include modulation of apoptosis, inhibition of inflammatory responses, reduction of IFN production, inhibition of autophagy, and suppression of MHC-I expression.  Insights into immunoevasion strategies by ASFV may shed light on the development of vaccines, as well as preventive and therapeutic drugs.
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12. Identification and genetic analysis of multiple P chromosomes of Agropyron cristatum in the background of common wheat
CHEN Hong-xin, HAN Hai-ming, LI Qing-feng, ZHANG Jin-peng, LU Yu-qing, YANG Xin-ming, LI Xiuquan, LIU Wei-hua, LI Li-hui
Journal of Integrative Agriculture    2018, 17 (08): 1697-1705.   DOI: 10.1016/S2095-3119(17)61861-6
摘要436)      PDF    收藏
Agropyron cristatum, a wild relative of common wheat (Triticum aestivum L.), provides many desirable genetic resources for wheat improvement, such as tolerance to cold, drought, and disease.  To transfer and utilize these desirable genes, in this study, two wheat-A. cristatum derivatives II-13 and II-23 were identified and analyzed.  We found that the number of root tip cell chromosomes was 44 in both II-13 and II-23, but there were four and six P genome chromosomes in II-13 and II-23, respectively, based on genomic in situ hybridization (GISH).  The chromosome configurations of II-13 and II-23 were both 2n=22II by the meiotic analysis of pollen mother cells (PMCs) at metaphase I, indicating that there were two and three pairs of P chromosomes in II-13 and II-23, respectively.  Notably, wheat chromosome 7D was absent in derivative line II-13 while II-23 lacked chromosomes 4B and 7A based on SSR analysis combining fluorescence in situ hybridization (FISH) analysis with pAs1 and pSc119.2 as probes.  Chromosomes 2P and 7P were detected in both II-13 and II-23.  Another pair of P genome chromosomes in II-23 was determined to be 4P based on expressed-sequences tags-sequence tagged sites (EST-STS) markers specific to A. cristatum and FISH with probes pAcTRT1 and pAcpCR2.  Overall, these results suggest that II-13 was a 7P (7D) substitution line with one pair of additional 2P chromosomes and II-23 was a multiple 4P (4B), 7P (7A) substitution line with one pair of additional 2P chromosomes.  Moreover, we obtained six alien disomic addition lines and five alien disomic substitution lines by backcrossing.  These new materials will allow desirable genes from A. cristatum to be used in common wheat.
 
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13. The genetic diversity analysis in the donkey myostatin gene
LIU Dong-hua, HAN Hao-yuan, ZHANG Xin, SUN Ting, LAN Xian-yong, CHEN Hong, LEI Chu-zhao, DANG Rui-hua
Journal of Integrative Agriculture    2017, 16 (03): 656-663.   DOI: 10.1016/S2095-3119(16)61445-4
摘要914)      PDF    收藏
Myostatin (MSTN) gene negatively controls skeletal muscle development and growth, variations of which play an important role in the regulation of skeletal muscle growth in mammals.  However, study on genetic polymorphism of MSTN gene in donkey is limited.  In this study, we screened the single nucleotide polymorphsims (SNPs) of MSTN gene in 13 Chinese donkey breeds.  Four novel SNPs (g.229T>C, g.872A>G, g.2014G>A, and g.2395C>G) were detected and genotyped by sequencing and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods.  Six haplotypes (H1–H6) were analyzed, which indicated abundant haplotype diversities in Chinese donkeys.  The haplotype H1 was the most dominant and ancient in all breeds.  Xinjiang donkey displayed the highest haplotype diversity.  The Neighbour-Joining (NJ) tree of MSTN gene among different species was constructed.  The clustering result of nine species was consistent with the fact of species differentiation.  Our results will provide a reliable theoretical basis for the preservation, exploration and utilization of Chinese donkey genetic resources.
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14. Effect of temperature on the development of sclerotia in Villosiclava virens
FAN Lin-lin, YONG Ming-li, LI Dan-yang, LIU Yi-jia, LAI Chao-hui, CHEN Hong-ming, CHENG Fang-min, HU Dong-wei
Journal of Integrative Agriculture    2016, 15 (11): 2550-2555.   DOI: 10.1016/S2095-3119(16)61400-4
摘要3004)      PDF    收藏
    The sclerotia of Villosiclava virens were found commonly in high altitude and the temperate regions, where the temperatures are relatively low in rice filling stage. To make sure if low temperature induce the sclerotial formation in V. virens, the inoculated rice panicles in laboratory and the diseased rice panicles cut from paddy fields were treated under different temperatures. The results showed that 3 days of night temperature at 15°C were enough to induce the sclerotial formation. The low temperature was much more effective for young balls with intact membranes. After appearance of chlamydospores on the ball surfaces, the sclerotium could not differentiate anymore. The sclerotia began to differentiate below the chlamydospore layer and gradually grew onto the ball surfaces. This suggests that low temperature in the early development stage of false smut balls is an important factor to induce the sclerotial differentiation, and rice cultivars with long growth periods are able to produce more sclerotium-bearing balls, which will produce mass of spores in paddy field in the coming year.
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15. Survey and examination of the potential alternative hosts of Villosiclava virens, the pathogen of rice false smut, in China
DENG Qi-de, YONG Ming-li, LI Dan-yang, LAI Chao-hui, CHEN Hong-ming, FAN Jing, HU Dong-wei
Journal of Integrative Agriculture    2015, 14 (7): 1332-1337.   DOI: 10.1016/S2095-3119(15)61030-9
摘要2021)      PDF    收藏
Rice false smut is caused by ascomycete Villosiclava virens, whose potential alternative hosts have been assumed previously. Here its potential alternative hosts were surveyed and identified from 2008 to 2013 in the main rice-growing regions in China. Two common weeds in paddy fields, Digitaria sanguinalis Scop. and Echinochloa crusgalli (L.) Beauv., were found to present the similar symptoms to smut diseases in a few individuals in 2012 and 2013 in Zhejiang and Sichuan provinces of China, respectively. After the examinations of the spore morphology, their infection and extension mode in hosts, pathogen cell wall components, and molecular identification, the two pathogens were identified to be the Basidiomycetes, Ustilago syntherismae and Ustilago trichophora, respectively. So far there has been no alternative host of V. virens to be identified in China. These suggest that the alternative hosts of V. virens, if they do exist, are not possible to play an important role in the pathogen life cycle and the disease epidemics.
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16. The complete genome sequence of Citrus vein enation virus from China
HUANG Ai-jun, SONG Zhen, CAO Meng-ji, CHEN Hong-ming, LI Zhong-an, ZHOU Chang-yong
Journal of Integrative Agriculture    2015, 14 (3): 598-601.   DOI: 10.1016/S2095-3119(14)60903-5
摘要2168)      PDF    收藏
The complete nucleotide sequence of an isolate of Citrus vein enation virus (CVEV-XZG) from China has been determined for the first time. The genome consisted of 5 983 nucleotides, coding for five open reading frames (ORFs), had a similar genomic organization features with Pea enation mosaic virus (PEMV). Nucleotide and deduced amino acid sequence identity of the five ORFs compared to isolate CVEV VE-1 range from 97.1 to 99.0% and 97.4 to 100.0%, these values compared to isolate PEMV-1 range from 45.2 to 51.6% and 31.1 to 45.2%. Phylogenetic analysis based on the complete genome sequence showed that the isolate CVEV-XZG had close relationship with Pea enation mosaic virus. The results supports CVEV may be a new member of genus Enamovirus. The full sequence of CVEV-XZG presented here may serve as a basis for future study of CVEV in China.
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17. Exploring the Novel Genetic Variant of PITX1 Gene and Its Effect on Milk Performance in Dairy Goats
LAN Xian-yong, ZHAO Hai-yu, LI Zhuan-jian, ZHOU Rui, PAN Chuan-ying, LEI Chu-zhao, CHEN Hong
Journal of Integrative Agriculture    2013, 12 (1): 118-126.   DOI: 10.1016/S2095-3119(13)60212-9
摘要1748)      PDF    收藏
Paired-like homeodomain transcription factor 1 (PITX1) plays an important role in pituitary development by indirectly regulating the expression of the GH and PRL genes, and therefore PITX1 gene is regarded as a potential candidate gene for building the relationship between the gene polymorphism and milk traits. The aim of this study was to explore the novel genetic variant in PITX1 gene and its effect on milk performance in dairy goats. Herein, a novel genetic variation (NW_00314033: g.201G>A or IVS1+41G>A) located at nt41 position of the first intron of the goat PITX1 gene was reported at the P1 locus, which can be genotyped by the Msp I PCR-RFLP. In the Msp I PCR-RFLP analyis, the GG variant was a major genotype, and the A variant was a minor allele in Guanzhong dairy goats which was at Hardy-Weinberg disequilibrium (chi-square c2=140, P<0.01). The establishment of associations between different genotypes and milk performance was performed in the analyzed population. A total of three significant associations of the polymorphism with average milk fat content (%) (P=0.045), morning milk fat content (%) (P=0.049), and afternoon milk fat content (%) (P=0.050), were found, respectively. A significant relationship between the polymorphism and average total solid content (P=0.029) was also detected. This novel single nucleotide polymorphism (SNP) extended the spectrum of genetic variation of the goat PITX1 gene, and its significant association with milk performance would benefit from the application of DNA markers related to improving milk performance through marker-assisted selection (MAS) in dairy goats.
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18. Novel 6-bp Deletion Mutation in visfatin Gene and Its Associations with Birth Weight and Bodyweight in Chinese Cattle
WANG Mou, ZHANG Ya, YU Hui, LAI Xin-sheng, ZHU Jin-long, JIAO Jin-zhen, LAN Xian-yong, LEI Chuzhao, ZHANG Liang-zhi, CHEN Hong
Journal of Integrative Agriculture    2012, 12 (8): 1327-1332.   DOI: 10.1016/S1671-2927(00)8662
摘要1377)      PDF    收藏
Visfatin, like insulin, induces phosphorylation of signal transduction proteins that operatate downstream of the insulin receptor. The present study is focused on detecting deletion of visfatin gene and analyzing its effect on growth traits in six Chinese cattle breeds (Nangyang, Luxi, Qinchuan, Jiaxian Red, Grassland Red, and Chinese Holstein) using DNA sequencing, PCR-SSCP and PCR-RFLP methods. For the first time, a 6-bp deletion of visfatin was described and two alleles were revealed: W and D. The χ2-test analysis demonstrated that all breeds were in agreement with Hardy-Weinberg equilibrium (P>0.05). The associations of the novel 6-bp deletion of visfatin gene with growth traits of Nanyang cattle at 6-, 12-, 18-, and 24-mon-old were analyzed. Birth weight, 12- and 24-mon-old cattle with genotype WW had greater birth weight and average daily gain than genotype WD (P<0.01 or P<0.05). These results suggest that the deletion may influence the birth weight and bodyweight in 12 mon-old cattle.
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19. An Insect Imaging System to Automate Rice Light-Trap Pest Identification
YAO Qing, LIU Qing-jie, YANG Bao-jun, CHEN Hong-ming, TANG Jian
Journal of Integrative Agriculture    2012, 12 (6): 978-985.   DOI: 10.1016/S1671-2927(00)8621
摘要1776)      PDF    收藏
Identification and counting of rice light-trap pests are important to monitor rice pest population dynamics and make pest forecast. Identification and counting of rice light-trap pests manually is time-consuming, and leads to fatigue and an increase in the error rate. A rice light-trap insect imaging system is developed to automate rice pest identification. This system can capture the top and bottom images of each insect by two cameras to obtain more image features. A method is proposed for removing the background by color difference of two images with pests and non-pests. 156 features including color, shape and texture features of each pest are extracted into an support vector machine (SVM) classifier with radial basis kernel function. The seven-fold cross-validation is used to improve the accurate rate of pest identification. Four species of Lepidoptera rice pests are tested and achieved 97.5% average accurate rate.
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20. Genetic Diversity and Structure of New Inbred Rice Cultivars in China
XU Qun, CHEN Hong, WANG Cai-hong, YU Han-yong, YUAN Xiao-ping, WANG Yi-ping, FENG Yue, TANG Sheng-xiang, WEI Xing-hua
Journal of Integrative Agriculture    2012, 12 (10): 1567-1573.   DOI: 10.1016/S1671-2927(00)8689
摘要1297)      PDF    收藏
A total of 408 inbred rice cultivars bred in the last decade were analyzed for 24 SSR markers. The results showed the genetic diversity of indica cultivars was higher than that of japonica cultivars, and the genetic diversity of new cultivars raised in recent years was lower. Among the six rice cropping regions (RCRs) in China, genetic diversity was the highest in the central rice region (RCR-II) and the southwest rice region (RCR-III). Genetic differences among subpopulations of japonica were more complex than those in indica. Differentiation among seasonal ecotypes and RCRs in indica populations was unclear, but differentiation between RCR-II and northeast rice region (RCR-V) was more distinct for japonica cultivars. Considering the North rice region (RCR-IV) has very low genetic diversity among the tested cultivars, it is important to broaden the genetic background for future cultivars in rice breeding programs.
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21. Single Nucleotide Polymorphism (SNP) Analysis of orexin Gene 5´ Regulatory Region in Chinese Indigenous Cattle Populations
ZHANG Ai-ling, ZHANG Li, ZHANG Liang-zhi, LAN Xian-yong, ZHANG Cun-lei, ZHANG Cun-fang, CHEN Hong
Journal of Integrative Agriculture    2011, 10 (8): 1273-1279.   DOI: 10.1016/S1671-2927(11)60119-3
摘要1997)      PDF    收藏
Orexin is an important neuropeptide that influences livestock’s appetite and food intake and is closely related withlivestock’s growth and development. The variations in the orexin gene 5´ regulatory region might have an influence onthe gene expression. Based on the hypothesis, five overlapped fragments including 1 794 bp of orexin gene 5´ regulatoryregion were investigated for single nucleotide polymorphisms (SNPs) by PCR-SSCP and DNA sequencing in threeindigenous cattle populations. A total of five SSCP patterns observed revealed ten SNPs in the region. Two SSCP patternsgenotypes (A and B) were exhibited in O-2 fragment and three (A, B and C) were found in O-5 fragment. O-2 contained fourSNPs, viz., -583 T>C, -479 C>T, -474 A>T, and -451 A>G. In another lous O-5, six SNPs were identified (-1 610 C>G,-1 585 G>A, -1 550 T>C, -1 548 A>C, -1 438 C>T, and -1 431 C>A). Seven SNPs were found in transcription factor bindingsites and four out of them existed in the core sequences. The SNPs at -479, -474 and -451 did not change the putativerecognition core sequences of their factors. But the mutation at -583 changed the binding sequence of EVI1 into NFA andcreated one new binding site for ZFHX simultaneously. In three populations, the frequencies of A, B and C genotypesof O-2 were 0.2367, 0.4842 and 0.2791, respectively. And the A pattern of O-5 was preponderant (0.7549) and the other Bpattern was not (0.2451). But the frequencies of different SSCP variants varied across three cattle populations. Bgenotype in O-2 had significant associations to body weight (BW) and daily weight gain (DWG) in Nanyang cattle 6- and12-mon aged and might serve as one potential candidate genetic marker for growth and development.
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22. Transgenic Pigs Carrying a Synthesized Fatty Acid Desaturase Gene Yield High Level of ω-3 PUFAs
REN Hong-yan, ZHENG Xin-min, CHEN Hong-xing , LI Kui
Journal of Integrative Agriculture    2011, 10 (10): 1603-1608.   DOI: 10.1016/S1671-2927(11)60157-0
摘要1706)      PDF    收藏
Polyunsaturated fatty acids (PUFAs) are essential for normal growth in mammals, especially the ω-3 PUFAs, which play important roles in preventing several life-threatening diseases, such as coronary heart disease and diabetes. In this study, we aimed to investigate whether the sFat-1 gene from Caenorhabditis briggsae could be functionally expressed in transgenic pigs, and whether the transgenic could synthesize high quality ω-3 PUFAs endogenously. In this study, a gene construct consisting of CMV promoter and 1.9 kb cDNA of ω-3 fatty acid desaturase gene (sFat-1) from C. briggsae was injected into the male pronucleus of pig embryos by microinjection. The piglets were screened for the transgene by PCR, Southern blot and reverse transcription-PCR analysis. Pigs that give positive results were mated with wild-type pigs to produce the next generation and the transmission of transgene was examined by PCR analysis. Fatty acids compositions of various tissues in the transgenic pigs were then analyzed by gas chromatograph. A total of 878 embryos were transferred into 42 recipients, among which 29 successfully got pregnant and gave birth to a total of 162 piglets, and 8 of them were identified to be transgenic. Fatty acid compositions in the transgenic pigs were altered, and the levels of ω-6: ω-3 ratios were decreased from 14.53 in the control to 2.62 in Fat-1 transgenic pigs. A number of primary sFat-1-transgenic pigs were bred in this study, which lays the foundation for cultivation of new varieties of transgenic pigs.
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23. 基于AR眼镜采集的叶片图像上潜叶蝇为害等级自动诊断方法
Zhongru Ye, Yongjian Liu, Fuyu Ye, Hang Li, Ju Luo, Jianyang Guo, Zelin Feng, Chen Hong, Lingyi Li, Shuhua Liu, Baojun Yang, Wanxue Liu, Qing Yao
Journal of Integrative Agriculture    DOI: 10.1016/j.jia.2025.02.008
录用日期: 2025-02-10