Novel 6-bp Deletion Mutation in visfatin Gene and Its Associations with Birth Weight and Bodyweight in Chinese Cattle

doi:10.1016/S1671-2927(00)8662

Journal of Integrative Agriculture ›› 2012, Vol. 12 ›› Issue (8): 1327-1332.DOI: 10.1016/S1671-2927(00)8662

Novel 6-bp Deletion Mutation in visfatin Gene and Its Associations with Birth Weight and Bodyweight in Chinese Cattle

WANG Mou, ZHANG Ya, YU Hui, LAI Xin-sheng, ZHU Jin-long, JIAO Jin-zhen, LAN Xian-yong, LEI Chuzhao, ZHANG Liang-zhi, CHEN Hong

Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University,Yangling 712100, P.R.China

收稿日期:2011-07-04 出版日期:2012-08-01 发布日期:2012-09-09
通讯作者: Correspondence CHEN Hong, E-mail: chenhong1212@263.net
基金资助:
This study was supported by the National Natural Science Foundation of China (30972080), the Keystone Project of Transfer Gene in China (2009ZX08009-157B), and the Program of National Beef Cattle Industrial Technology System of China (CARS-38).

Novel 6-bp Deletion Mutation in visfatin Gene and Its Associations with Birth Weight and Bodyweight in Chinese Cattle

WANG Mou, ZHANG Ya, YU Hui, LAI Xin-sheng, ZHU Jin-long, JIAO Jin-zhen, LAN Xian-yong, LEI Chuzhao, ZHANG Liang-zhi, CHEN Hong

Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University,Yangling 712100, P.R.China

Received:2011-07-04 Online:2012-08-01 Published:2012-09-09
Contact: Correspondence CHEN Hong, E-mail: chenhong1212@263.net
Supported by:
This study was supported by the National Natural Science Foundation of China (30972080), the Keystone Project of Transfer Gene in China (2009ZX08009-157B), and the Program of National Beef Cattle Industrial Technology System of China (CARS-38).

摘要/Abstract

摘要： Visfatin, like insulin, induces phosphorylation of signal transduction proteins that operatate downstream of the insulin receptor. The present study is focused on detecting deletion of visfatin gene and analyzing its effect on growth traits in six Chinese cattle breeds (Nangyang, Luxi, Qinchuan, Jiaxian Red, Grassland Red, and Chinese Holstein) using DNA sequencing, PCR-SSCP and PCR-RFLP methods. For the first time, a 6-bp deletion of visfatin was described and two alleles were revealed: W and D. The χ2-test analysis demonstrated that all breeds were in agreement with Hardy-Weinberg equilibrium (P>0.05). The associations of the novel 6-bp deletion of visfatin gene with growth traits of Nanyang cattle at 6-, 12-, 18-, and 24-mon-old were analyzed. Birth weight, 12- and 24-mon-old cattle with genotype WW had greater birth weight and average daily gain than genotype WD (P<0.01 or P<0.05). These results suggest that the deletion may influence the birth weight and bodyweight in 12 mon-old cattle.

关键词: visfatin, deletion, mutation, cattle

Abstract: Visfatin, like insulin, induces phosphorylation of signal transduction proteins that operatate downstream of the insulin receptor. The present study is focused on detecting deletion of visfatin gene and analyzing its effect on growth traits in six Chinese cattle breeds (Nangyang, Luxi, Qinchuan, Jiaxian Red, Grassland Red, and Chinese Holstein) using DNA sequencing, PCR-SSCP and PCR-RFLP methods. For the first time, a 6-bp deletion of visfatin was described and two alleles were revealed: W and D. The χ2-test analysis demonstrated that all breeds were in agreement with Hardy-Weinberg equilibrium (P>0.05). The associations of the novel 6-bp deletion of visfatin gene with growth traits of Nanyang cattle at 6-, 12-, 18-, and 24-mon-old were analyzed. Birth weight, 12- and 24-mon-old cattle with genotype WW had greater birth weight and average daily gain than genotype WD (P<0.01 or P<0.05). These results suggest that the deletion may influence the birth weight and bodyweight in 12 mon-old cattle.

Key words: visfatin, deletion, mutation, cattle

WANG Mou, ZHANG Ya, YU Hui, LAI Xin-sheng, ZHU Jin-long, JIAO Jin-zhen, LAN Xian-yong, LEI Chuzhao, ZHANG Liang-zhi, CHEN Hong. Novel 6-bp Deletion Mutation in visfatin Gene and Its Associations with Birth Weight and Bodyweight in Chinese Cattle[J]. Journal of Integrative Agriculture, 2012, 12(8): 1327-1332.

参考文献

[1]Berndt J, Kloting N, Kralisch S, Kovacs P, Fasshauer M, Schon M R, Stumvoll M, Bluher M. 2005. Plasma visfatin concentrations and fat depot-specific expression in humans. Diabetes, 54, 2911-2916.

[2]Berulava T, Horsthemke B. 2010. The obesity-associated SNPs in intron 1 of the FTO gene affect primary transcript levels. European Journal of Human Genetics, 18, 1054-1056.

[3]Bland J M, Altman D G. 1995. Multiple significance tests: the bonferroni method. British Medical Journal, 310, 170-170.

[4]Bottcher Y, Teupser D, Enigk B, Berndt J, Kloting N, Schon M R, Thiery J, Bluher M, Stumvoll M, Kovacs P. 2006. Genetic variation in the visfatin gene (PBEF1) and its relation to glucose metabolism and fat-depot-specific messenger ribonucleic acid expression in humans. Journal of Clinical Endocrinology & Metabolism, 91, 2725-2731.

[5]Botstein D, White R L, Skolnick M, Davis R W. 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American Journal of Human Genetics, 32, 314-331.

[6]Christopher H, Harvey F. 2005. Lodish visfatin: a new adipokine. Science, 21, 366-367.

[7]Dominik G H, Ammon H, Angela S, Elena V. 2007. Visfatin response to glucose is reduced in women with gestational diabetes mellitus diabetes care. Agriculture Journals, 307, 1889-1891.

[8]Fukuhara A, Matsuda M, Nishizawa M, Segawa K, Tanaka M, Kishimoto K, Matsuki Y, Murakami M, Ichisaka T, Murakami H, et al. 2005. Visfatin: A protein secreted by visceral fat that mimics the effects of insulin. Science, 307, 426-430.

[9]Greenwood T A, Kelsoe J R. 2003. Promoter and intronic variants affect the transcriptional regulation of the human dopamine transporter gene. Genomics, 82, 511-520.

[10]Han R L, Lan X Y, Zhang L Z, Ren G, Jing Y J, Li M J, Zhang B, Zhao M, Guo Y K, Kang X T, et al. 2010. A novel single-nucleotide polymorphism of the visfatin gene and its associations with performance traits in the chicken. Journal of Applied Genetics, 51, 59-65.

[11]Han R L, Li Z J, Li M J, Li J Q, Lan X Y, Sun G R, Kang X T, Chen H. 2011. Novel 9-bp indel in visfatin gene and its associations with chicken growth. British Poultry Science, 52, 52-57.

[12]Kershaw E E, Flier J S. 2004. Adipose tissue as an endocrine organ. Journal of Clinical Endocrinology and Metabolism, 89, 2548-2556.

[13]Kloting N, Kloting I. 2005. Visfatin: gene expression in isolated adipocytes and sequence analysis in obese WOKW rats compared with lean control rats. B i o c h e m i c a l a n d B i o p h y s i c a l R e s e a r c h Communications, 332, 1070-1072.

[14]Krzysik-Walker S M, Ocon-Grove O M, Maddineni S R. 2008. Is visfatin an adipokine or myokine? Evidence for greater visfatin expression in skeletal muscle than visceral fat in chickens. Endocrinology, 149, 1543-1550.

[15]Le H H, Nott A, Moore M J. 2003. How introns influence and enhance eukaryotic gene expression. Trends in Biochemical Sciences, 28, 215-220.

[16]Lemor A, Mielenz M, Altmann M. 2010. mRNA abundance of adiponectin and its receptors, leptin and visfatin and of G-protein coupled receptor 41 in five different fat depots from sheep. Journal of Animal Physiology and Animal Nutrition, 94, e96-e101. Mistry T, Digby J E, Raghu R, Joe J, Desai K M, Randeva H S. 2008. A novel role for the adipokine visfatin (pre-B cell colony-enhancing factor 1 (PBEF)/nicotinamide phosphoribosyltransferase (NMPRTase)) in prostate carcinogenesis. European Urology Supplements, 7, 964. Nei M, Li W H. 1979. Mathematic model for studying genetic variation in terms of restriction endonucleaes. Proceedings of the National Academy of Sciences of the United States of America, 76, 5269-5273.

[17]Ons E, Gertler A, Buyse J. 2010. Visfatin gene expression in chickens is sex and tissue dependent. Domestic Animal Endocrinology, 38, 63-74.

[18]Palin M F, Labrecque B, Beaudry D, Mayhue M, Bordignon V, Murphy B D. 2008. Visfatin expression is not associated with adipose tissue abundance in the porcine model. Domestic Animal Endocrinology, 35, 58-73.

[19]Sethi J K, Vidal-Puig A. 2005. Visfatin: the missing link between intra-abdominal obesity and diabetes? Trends in Molecular Medicine, 11, 344-347.

[20]Skop V, Kontrova K, Zidek V, Pravenec M, Kazdova L, Mikulik K, Sajdok J, Zidkova J. 2010. Autocrine effects of visfatin on hepatocyte sensitivity to insulin action. Physiological Research, 59, 615-618.

[21]Sambrook J R D. 2002. Molecular Cloning: A Laboratory Manual. 3rd ed. China Science Press, Beijing, China. Walters R G, Jacquemont S, Valsesia A, de Smith A J, Martinet D, Andersson J. 2010. A new highly penetrant form of obesity due to deletions on chromosome 16p11. 2. Nature, 463, 671-677.

[22]Wang M, Yu H, Chen H, Lan X Y, Zhang L Z, Zhao M, Lai X S, Wang X L, Wang K Y, Lei C Z, et al. 2009. Novel 35-bp insertion in visfatin gene in Chinese cattle. Molecular Biology, 43, 557-561.

[23]Xie H, Tang S Y, Luo X H, Huang J, Cui R R, Yuan L Q, Zhou H D, Wu X P, Liao E Y. 2007. Insulin-like effects of visfatin on human osteoblasts. Calcified Tissue International, 80, 201-210.

[24]Zrustova J, Knoll A, Urban T. 2009. The visfatin (NAMPT; PBEF1) gene polymorphisms and associations with meat performance traits in three pig breeds kept in the Czech Republic. Czech Journal of Animal Science, 54, 443-447.

Novel 6-bp Deletion Mutation in visfatin Gene and Its Associations with Birth Weight and Bodyweight in Chinese Cattle

Novel 6-bp Deletion Mutation in visfatin Gene and Its Associations with Birth Weight and Bodyweight in Chinese Cattle

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 8

编辑推荐

Metrics

[1]	MENG Qing-feng, LI Da-wei, ZHANG Juan, ZHOU Lian-ren, MA Xian-fa, WANG Hong-yan, WANG Guang-cheng. Soil properties and corn (Zea mays L.) production under manure application combined with deep tillage management in solonetzic soils of Songnen Plain, Northeast China[J]. Journal of Integrative Agriculture, 2016, 15(4): 879-890.
[2]	ZHAI Hui-jie, FENG Zhi-yu, LIU Xin-ye, CHENG Xue-jiao, PENG Hui-ru, YAO Ying-yin, SUN Qi-xin, NI Zhong-fu. A genetic linkage map with 178 SSR and 1 901 SNP markers constructed using a RIL population in wheat (Triticum aestivum L.)[J]. Journal of Integrative Agriculture, 2015, 14(9): 1697-1705.
[3]	ZHANG Yong-qiang, LIU Hai-sheng, WU Xiao-dong, WANG Xiao-zhen, LI Jin-ming, ZHAO Yonggang, Lü Yan, REN Wei-jie, GE Sheng-qiang, WANG Zhi-liang. A novel real-time RT-PCR with TaqMan-MGB probes and its application in detecting BVDV infections in dairy farms[J]. Journal of Integrative Agriculture, 2015, 14(8): 1637-1643.
[4]	WU Lei, JIA Yan-li, WU Gang, LU Chang-ming. Molecular evidence for blocking erucic acid synthesis in rapeseed (Brassica napus L.) by a two-base-pair deletion in FAE1 (fatty acid elongase 1)[J]. Journal of Integrative Agriculture, 2015, 14(7): 1251-1260.
[5]	ZHAO Shan-jiang, ZHAO Xue-ming, DU Wei-hua, HAO Hai-sheng, LIU Yan, QIN Tong, WANG Dong. Production of early monozygotic twin bovine embryos in vitro by the blastomere separation and coculture technique[J]. Journal of Integrative Agriculture, 2015, 14(10): 2034-2041.
[6]	LI Ling-ling , LI Shu-tian. Nitrogen Mineralization from Animal Manures and Its Relation to Organic N Fractions[J]. Journal of Integrative Agriculture, 2014, 13(9): 2040-2048.
[7]	LI Ming-gui, LIU Zhen-shan, PAN Zeng-xiang, LUO Hua, XIE Zhuang,LI Qi-fa. The mRNA Expression and Methylation Status in Imprinting Control Region of H19 Gene Between Cattle-Yak and Their Parents[J]. Journal of Integrative Agriculture, 2012, 12(10): 1691-1699.
[8]	ZHANG Ai-ling, ZHANG Li, ZHANG Liang-zhi, LAN Xian-yong, ZHANG Cun-lei, ZHANG Cun-fang, CHEN Hong. Single Nucleotide Polymorphism (SNP) Analysis of orexin Gene 5´ Regulatory Region in Chinese Indigenous Cattle Populations[J]. Journal of Integrative Agriculture, 2011, 10(8): 1273-1279.