Exploring the Novel Genetic Variant of PITX1 Gene and Its Effect on Milk Performance in Dairy Goats

doi:10.1016/S2095-3119(13)60212-9

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (1): 118-126.DOI: 10.1016/S2095-3119(13)60212-9

Exploring the Novel Genetic Variant of PITX1 Gene and Its Effect on Milk Performance in Dairy Goats

LAN Xian-yong, ZHAO Hai-yu, LI Zhuan-jian, ZHOU Rui, PAN Chuan-ying, LEI Chu-zhao, CHEN Hong

1.College of Animal Science and Technology, Northwest A&F University/Shaanxi Key Laboratory of Molecular Biology for Agriculture,Yangling 712100, P.R.China
2.College of Life Sciences, Northwest A&F University, Yangling 712100, P.R.China

收稿日期:2012-03-02 出版日期:2013-01-01 发布日期:2013-01-24
通讯作者: Correspondence CHEN Hong, Tel: +86-29-87092004, Fax: +86-29-87092164, E-mail: chenhong1212@263.net
作者简介:LAN Xian-yong, Tel: +86-29-87092004, Fax: +86-29-87092164, E-mail: lanxianyong79@yahoo.com.cn; ZHAO Hai-yu, Tel: +86-29-87092004, Fax: +86-29-87092164, E-mail: haiyuzhao616@gmail.com;
基金资助:
This paper was funded by the National Natural Science Foundation of China (31172184), the Young New Star Project on Science & Technology of Shaanxi Province, China (2011kjxx64), the Natural Science Foundation of Shaanxi Province of China (2011JQ3009), the Young Topnotch Researcher Support Project of Northwest A&F University, China (QNGG-2009-007), and the Special Fund for Basic Scientific Research and Operation Expenses in Sci-Tech Innovation of Northwest A&F University, China (QN2011102).

Exploring the Novel Genetic Variant of PITX1 Gene and Its Effect on Milk Performance in Dairy Goats

LAN Xian-yong, ZHAO Hai-yu, LI Zhuan-jian, ZHOU Rui, PAN Chuan-ying, LEI Chu-zhao, CHEN Hong

1.College of Animal Science and Technology, Northwest A&F University/Shaanxi Key Laboratory of Molecular Biology for Agriculture,Yangling 712100, P.R.China
2.College of Life Sciences, Northwest A&F University, Yangling 712100, P.R.China

Received:2012-03-02 Online:2013-01-01 Published:2013-01-24
Contact: Correspondence CHEN Hong, Tel: +86-29-87092004, Fax: +86-29-87092164, E-mail: chenhong1212@263.net
About author:LAN Xian-yong, Tel: +86-29-87092004, Fax: +86-29-87092164, E-mail: lanxianyong79@yahoo.com.cn; ZHAO Hai-yu, Tel: +86-29-87092004, Fax: +86-29-87092164, E-mail: haiyuzhao616@gmail.com;
Supported by:
This paper was funded by the National Natural Science Foundation of China (31172184), the Young New Star Project on Science & Technology of Shaanxi Province, China (2011kjxx64), the Natural Science Foundation of Shaanxi Province of China (2011JQ3009), the Young Topnotch Researcher Support Project of Northwest A&F University, China (QNGG-2009-007), and the Special Fund for Basic Scientific Research and Operation Expenses in Sci-Tech Innovation of Northwest A&F University, China (QN2011102).

摘要/Abstract

摘要： Paired-like homeodomain transcription factor 1 (PITX1) plays an important role in pituitary development by indirectly regulating the expression of the GH and PRL genes, and therefore PITX1 gene is regarded as a potential candidate gene for building the relationship between the gene polymorphism and milk traits. The aim of this study was to explore the novel genetic variant in PITX1 gene and its effect on milk performance in dairy goats. Herein, a novel genetic variation (NW_00314033: g.201G>A or IVS1+41G>A) located at nt41 position of the first intron of the goat PITX1 gene was reported at the P1 locus, which can be genotyped by the Msp I PCR-RFLP. In the Msp I PCR-RFLP analyis, the GG variant was a major genotype, and the A variant was a minor allele in Guanzhong dairy goats which was at Hardy-Weinberg disequilibrium (chi-square c2=140, P<0.01). The establishment of associations between different genotypes and milk performance was performed in the analyzed population. A total of three significant associations of the polymorphism with average milk fat content (%) (P=0.045), morning milk fat content (%) (P=0.049), and afternoon milk fat content (%) (P=0.050), were found, respectively. A significant relationship between the polymorphism and average total solid content (P=0.029) was also detected. This novel single nucleotide polymorphism (SNP) extended the spectrum of genetic variation of the goat PITX1 gene, and its significant association with milk performance would benefit from the application of DNA markers related to improving milk performance through marker-assisted selection (MAS) in dairy goats.

关键词: dairy goat , PITX1 gene , genetic variant , association , milk performance

Abstract: Paired-like homeodomain transcription factor 1 (PITX1) plays an important role in pituitary development by indirectly regulating the expression of the GH and PRL genes, and therefore PITX1 gene is regarded as a potential candidate gene for building the relationship between the gene polymorphism and milk traits. The aim of this study was to explore the novel genetic variant in PITX1 gene and its effect on milk performance in dairy goats. Herein, a novel genetic variation (NW_00314033: g.201G>A or IVS1+41G>A) located at nt41 position of the first intron of the goat PITX1 gene was reported at the P1 locus, which can be genotyped by the Msp I PCR-RFLP. In the Msp I PCR-RFLP analyis, the GG variant was a major genotype, and the A variant was a minor allele in Guanzhong dairy goats which was at Hardy-Weinberg disequilibrium (chi-square c2=140, P<0.01). The establishment of associations between different genotypes and milk performance was performed in the analyzed population. A total of three significant associations of the polymorphism with average milk fat content (%) (P=0.045), morning milk fat content (%) (P=0.049), and afternoon milk fat content (%) (P=0.050), were found, respectively. A significant relationship between the polymorphism and average total solid content (P=0.029) was also detected. This novel single nucleotide polymorphism (SNP) extended the spectrum of genetic variation of the goat PITX1 gene, and its significant association with milk performance would benefit from the application of DNA markers related to improving milk performance through marker-assisted selection (MAS) in dairy goats.

Key words: dairy goat , PITX1 gene , genetic variant , association , milk performance

LAN Xian-yong, ZHAO Hai-yu, LI Zhuan-jian, ZHOU Rui, PAN Chuan-ying, LEI Chu-zhao, CHEN Hong. Exploring the Novel Genetic Variant of PITX1 Gene and Its Effect on Milk Performance in Dairy Goats[J]. Journal of Integrative Agriculture, 2013, 12(1): 118-126.

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Exploring the Novel Genetic Variant of PITX1 Gene and Its Effect on Milk Performance in Dairy Goats

Exploring the Novel Genetic Variant of PITX1 Gene and Its Effect on Milk Performance in Dairy Goats

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