Regulatory Function of Polyamine Oxidase-Generated Hydrogen Peroxide in Ethylene-Induced Stomatal Closure in Arabidopsis thaliana

doi:10.1016/S2095-3119(13)60224-5

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (2): 251-262.DOI: 10.1016/S2095-3119(13)60224-5

Regulatory Function of Polyamine Oxidase-Generated Hydrogen Peroxide in Ethylene-Induced Stomatal Closure in Arabidopsis thaliana

HOU Zhi-hui, LIU Guo-hua, HOU Li-xia, WANG Lan-xiang , LIU Xin

College of Life Sciences, Qingdao Agricultural University/University Key Laboratory of Plant Biotechnology in Shandong Province, Universities of Shandong, Qingdao 266109, P.R.China

收稿日期:2012-01-09 出版日期:2013-02-01 发布日期:2013-02-06
通讯作者: Correspondence LIU Xin, Tel: +86-532-88030224, Fax: +86-532-86080640, E-mail: liuxin6080@yahoo.com.cn
基金资助:
This study was supported by the National Natural Science Foundation of China (30970228 and 31170237), the National Science Foundation of Shandong Province, China (ZR2010CM024), and the Foundation of State Key Laboratory of Plant Physiology and Biochemistry, China (SKLPPBKF11001).

Regulatory Function of Polyamine Oxidase-Generated Hydrogen Peroxide in Ethylene-Induced Stomatal Closure in Arabidopsis thaliana

HOU Zhi-hui, LIU Guo-hua, HOU Li-xia, WANG Lan-xiang , LIU Xin

College of Life Sciences, Qingdao Agricultural University/University Key Laboratory of Plant Biotechnology in Shandong Province, Universities of Shandong, Qingdao 266109, P.R.China

Received:2012-01-09 Online:2013-02-01 Published:2013-02-06
Contact: Correspondence LIU Xin, Tel: +86-532-88030224, Fax: +86-532-86080640, E-mail: liuxin6080@yahoo.com.cn
Supported by:
This study was supported by the National Natural Science Foundation of China (30970228 and 31170237), the National Science Foundation of Shandong Province, China (ZR2010CM024), and the Foundation of State Key Laboratory of Plant Physiology and Biochemistry, China (SKLPPBKF11001).

摘要/Abstract

摘要： Hydrogen peroxide (H2O2) is an important signaling molecule in ethylene-induced stomatal closure in Arabidopsis thaliana. Early studies on the sources of H2O2 mainly focused on NADPH oxidases and cell-wall peroxidases. Here, we report the involvement of polyamine oxidases (PAOs) in ethylene-induced H2O2 production in guard cells. In Arabidopsis epidermal peels, application of PAO inhibitors caused the failure of ethylene to induce H2O2 production and stomatal closure. Results of quantitative RT-PCR analysis and pharmacological experiments showed that AtPAO2 and AtPAO4 transcripts and activities of PAOs were both induced by ethylene. In transgenic Arabidopsis plants over-expressing AtPAO2 and AtPAO4, stomatal movement was more sensitive to ethylene treatment and H2O2 production was also significantly induced. The increased H2O2 production in the transgenic lines compared to the wild-type plants suggests that AtPAO2 and AtPAO4 probably are involved in ethylene-induced H2O2 production. Several factors which induce stomatal closure such as dehydration and high salinity all enhanced the expression of AtPAO2 and AtPAO4 to different degrees. Moreover, GFPAtPAOs fusion protein localized in the nucleus, cytoplasm, and cell wall of the guard cells. Therefore, our results strongly indicated that PAO is a source of H2O2 generation in Arabidopsis guard cells and plays crucial roles in stomatal movement.

关键词: Arabidopsis thaliana , ethylene , guard cell , hydrogen peroxide , polyamine oxidase

Abstract: Hydrogen peroxide (H2O2) is an important signaling molecule in ethylene-induced stomatal closure in Arabidopsis thaliana. Early studies on the sources of H2O2 mainly focused on NADPH oxidases and cell-wall peroxidases. Here, we report the involvement of polyamine oxidases (PAOs) in ethylene-induced H2O2 production in guard cells. In Arabidopsis epidermal peels, application of PAO inhibitors caused the failure of ethylene to induce H2O2 production and stomatal closure. Results of quantitative RT-PCR analysis and pharmacological experiments showed that AtPAO2 and AtPAO4 transcripts and activities of PAOs were both induced by ethylene. In transgenic Arabidopsis plants over-expressing AtPAO2 and AtPAO4, stomatal movement was more sensitive to ethylene treatment and H2O2 production was also significantly induced. The increased H2O2 production in the transgenic lines compared to the wild-type plants suggests that AtPAO2 and AtPAO4 probably are involved in ethylene-induced H2O2 production. Several factors which induce stomatal closure such as dehydration and high salinity all enhanced the expression of AtPAO2 and AtPAO4 to different degrees. Moreover, GFPAtPAOs fusion protein localized in the nucleus, cytoplasm, and cell wall of the guard cells. Therefore, our results strongly indicated that PAO is a source of H2O2 generation in Arabidopsis guard cells and plays crucial roles in stomatal movement.

Key words: Arabidopsis thaliana , ethylene , guard cell , hydrogen peroxide , polyamine oxidase

HOU Zhi-hui, LIU Guo-hua, HOU Li-xia, WANG Lan-xiang , LIU Xin. Regulatory Function of Polyamine Oxidase-Generated Hydrogen Peroxide in Ethylene-Induced Stomatal Closure in Arabidopsis thaliana[J]. Journal of Integrative Agriculture, 2013, 12(2): 251-262.

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Regulatory Function of Polyamine Oxidase-Generated Hydrogen Peroxide in Ethylene-Induced Stomatal Closure in Arabidopsis thaliana

Regulatory Function of Polyamine Oxidase-Generated Hydrogen Peroxide in Ethylene-Induced Stomatal Closure in Arabidopsis thaliana

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