Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (8): 1431-1440.DOI: 10.1016/S1671-2927(00)9057

• 论文 • 上一篇    下一篇

Identification of Differentially Expressed Genes During Ethylene Climacteric of Melon Fruit by Suppression Subtractive Hybridization

 GAO Feng, NIU Yi-ding, HAO Jin-feng, BADE Rengui, ZHANG Li-quan , HASI Agula   

  1. Inner Mongolia Key Laboratory of Herbage & Endemic Crop Biotechnology/College of Life Sciences, Inner Mongolia University, Hohhot 010021, P.R.China
  • 收稿日期:2012-09-18 出版日期:2013-08-01 发布日期:2013-09-12
  • 通讯作者: Correspondence HASI Agula, Tel: +86-471-4992209, Fax: +86-471-4992435, E-mail: hasind@sina.com
  • 作者简介:GAO Feng, E-mail: imgaofeng@163.com
  • 基金资助:

    This work was supported by the National Natural Science Foundation of China (30960159) and the Specialized Research Foundation for the Doctoral Program of Higher Education (200801260002).

Identification of Differentially Expressed Genes During Ethylene Climacteric of Melon Fruit by Suppression Subtractive Hybridization

 GAO Feng, NIU Yi-ding, HAO Jin-feng, BADE Rengui, ZHANG Li-quan , HASI Agula   

  1. Inner Mongolia Key Laboratory of Herbage & Endemic Crop Biotechnology/College of Life Sciences, Inner Mongolia University, Hohhot 010021, P.R.China
  • Received:2012-09-18 Online:2013-08-01 Published:2013-09-12
  • Contact: Correspondence HASI Agula, Tel: +86-471-4992209, Fax: +86-471-4992435, E-mail: hasind@sina.com
  • About author:GAO Feng, E-mail: imgaofeng@163.com
  • Supported by:

    This work was supported by the National Natural Science Foundation of China (30960159) and the Specialized Research Foundation for the Doctoral Program of Higher Education (200801260002).

摘要: Melon (Cucumis melo L.) is an important horticultural crop worldwide. Ethylene regulates the ripening process and affects the ripening rate. To screen genes that are differentially expressed at the burst of ethylene climacteric in melon fruit, we performed suppression subtractive hybridization (SSH) to generate forward and reverse libraries, for which we sequenced 439 and 445 clones, respectively. Our BLAST analysis showed that the genes from the 2 libraries were involved in metabolism, signal transduction, cell structure, transcription, translation, and defense. Six genes were analyzed by qRT-PCR during the differential developmental stage of melon fruit. Our results provide new insight into the understanding of climacteric ripening of melon fruit.

关键词: Cucumis melo , ethylene climacteric , fruit ripening , differentially expressed mRNA , gene expression , suppression subtractive hybridization

Abstract: Melon (Cucumis melo L.) is an important horticultural crop worldwide. Ethylene regulates the ripening process and affects the ripening rate. To screen genes that are differentially expressed at the burst of ethylene climacteric in melon fruit, we performed suppression subtractive hybridization (SSH) to generate forward and reverse libraries, for which we sequenced 439 and 445 clones, respectively. Our BLAST analysis showed that the genes from the 2 libraries were involved in metabolism, signal transduction, cell structure, transcription, translation, and defense. Six genes were analyzed by qRT-PCR during the differential developmental stage of melon fruit. Our results provide new insight into the understanding of climacteric ripening of melon fruit.

Key words: Cucumis melo , ethylene climacteric , fruit ripening , differentially expressed mRNA , gene expression , suppression subtractive hybridization