Effects of NaCl and Iso-Osmotic Polyethylene Glycol Stress on Na+/H+ Antiport Activity of Three Malus species with Different Salt Tolerance

doi:10.1016/S2095-3119(13)60627-9

Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (6): 1276-1283.DOI: 10.1016/S2095-3119(13)60627-9

Effects of NaCl and Iso-Osmotic Polyethylene Glycol Stress on Na+/H+ Antiport Activity of Three Malus species with Different Salt Tolerance

YANG Hong-bing, DONG Chun-hai, XU Xue-feng, WANG Yi , HAN Zhen-hai

1、Key Lab of Plant Biotechnology in Universities of Shandong/College of Life Sciences, Qingdao Agricultural University, Qingdao 266109,
P.R.China
2、Stress Physiology and Molecular Biology Laboratory of Fruit Trees, China Agricultural University, Beijing 100193, P.R.China

收稿日期:2013-05-28 出版日期:2014-06-01 发布日期:2014-06-06
通讯作者: HAN Zhen-hai, Tel: +86-10-62736880, E-mail: rschan@cau.edu.cn
作者简介:YANG Hong-bing, Tel: +86-532-88030995, E-mail: hbyang@qau.edu.cn
基金资助:
The authors would like to acknowledge the financial support from the National Natural Science Foundation of China (39740027) and the Special Fund for Agro-Scientific Research in the Public Interest of China (201203075).

Effects of NaCl and Iso-Osmotic Polyethylene Glycol Stress on Na+/H+ Antiport Activity of Three Malus species with Different Salt Tolerance

YANG Hong-bing, DONG Chun-hai, XU Xue-feng, WANG Yi , HAN Zhen-hai

1、Key Lab of Plant Biotechnology in Universities of Shandong/College of Life Sciences, Qingdao Agricultural University, Qingdao 266109,
P.R.China
2、Stress Physiology and Molecular Biology Laboratory of Fruit Trees, China Agricultural University, Beijing 100193, P.R.China

Received:2013-05-28 Online:2014-06-01 Published:2014-06-06
Contact: HAN Zhen-hai, Tel: +86-10-62736880, E-mail: rschan@cau.edu.cn
About author:YANG Hong-bing, Tel: +86-532-88030995, E-mail: hbyang@qau.edu.cn
Supported by:
The authors would like to acknowledge the financial support from the National Natural Science Foundation of China (39740027) and the Special Fund for Agro-Scientific Research in the Public Interest of China (201203075).

摘要/Abstract

摘要： Salt stress contains osmotic and ionic stress, while iso-osmotic polyethylene glycol (PEG) has only osmotic stress. This study aimed to compare the different effects on the activity of H+-ATPase, proton pump and Na+/H+ antiport in Malus seedlings between osmotic and ionic stress. Species of salt tolerant Malus zumi, middle salt tolerant Malus xiaojinensis and salt sensitive Malus baccata were used as experimental materials. Malus seedlings were treated with NaCl and iso-osmotic PEG stress. The activity of H+-ATPase, proton pump and Na+/H+ antiport of plasmolemma and tonoplast in Malus seedlings were obviously increased under salt stress, and those in salt-tolerant species increased more. Under the same NaCl concentration, the activity of H+- ATPase, proton pump and Na+/H+ antiport of plasmolemma and tonoplast in salt-tolerant species were all obviously higher than those in salt-sensitive one. Higher Na+/H+ antiport activity of plasmolemma and tonoplast in salt-tolerant species could help to extrude and compartmentalize sodium in roots under salt stress. The ascent rate of activity of H+-ATPase, proton pump and Na+/ H+ antiport in Malus seedlings under the three salt concentration stress was all obviously higher than that under the iso-osmotic PEG stress. It indicated that the sodium ion effect had more stimulation on the activity of H+-ATPase, proton pump and Na+/H+ antiport in salt-tolerant species, and salt-tolerant species has higher capability of sodium extrusion and compartmentalization in roots and is therefore more salt tolerant.

关键词: Malus , NaCl , polyethylene glycol (PEG) , Na+/H+ antiport activity , salt tolerance

Abstract: Salt stress contains osmotic and ionic stress, while iso-osmotic polyethylene glycol (PEG) has only osmotic stress. This study aimed to compare the different effects on the activity of H+-ATPase, proton pump and Na+/H+ antiport in Malus seedlings between osmotic and ionic stress. Species of salt tolerant Malus zumi, middle salt tolerant Malus xiaojinensis and salt sensitive Malus baccata were used as experimental materials. Malus seedlings were treated with NaCl and iso-osmotic PEG stress. The activity of H+-ATPase, proton pump and Na+/H+ antiport of plasmolemma and tonoplast in Malus seedlings were obviously increased under salt stress, and those in salt-tolerant species increased more. Under the same NaCl concentration, the activity of H+- ATPase, proton pump and Na+/H+ antiport of plasmolemma and tonoplast in salt-tolerant species were all obviously higher than those in salt-sensitive one. Higher Na+/H+ antiport activity of plasmolemma and tonoplast in salt-tolerant species could help to extrude and compartmentalize sodium in roots under salt stress. The ascent rate of activity of H+-ATPase, proton pump and Na+/ H+ antiport in Malus seedlings under the three salt concentration stress was all obviously higher than that under the iso-osmotic PEG stress. It indicated that the sodium ion effect had more stimulation on the activity of H+-ATPase, proton pump and Na+/H+ antiport in salt-tolerant species, and salt-tolerant species has higher capability of sodium extrusion and compartmentalization in roots and is therefore more salt tolerant.

Key words: Malus , NaCl , polyethylene glycol (PEG) , Na+/H+ antiport activity , salt tolerance

YANG Hong-bing, DONG Chun-hai, XU Xue-feng, WANG Yi , HAN Zhen-hai. Effects of NaCl and Iso-Osmotic Polyethylene Glycol Stress on Na+/H+ Antiport Activity of Three Malus species with Different Salt Tolerance[J]. Journal of Integrative Agriculture, 2014, 13(6): 1276-1283.

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Effects of NaCl and Iso-Osmotic Polyethylene Glycol Stress on Na+/H+ Antiport Activity of Three Malus species with Different Salt Tolerance

Effects of NaCl and Iso-Osmotic Polyethylene Glycol Stress on Na+/H+ Antiport Activity of Three Malus species with Different Salt Tolerance

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