Hydrogen Sulfide May Function Downstream of Nitric Oxide in Ethylene- Induced Stomatal Closure in Vicia faba L.

doi:10.1016/S1671-2927(00)8697

Journal of Integrative Agriculture ›› 2012, Vol. 12 ›› Issue (10): 1644-1653.DOI: 10.1016/S1671-2927(00)8697

Hydrogen Sulfide May Function Downstream of Nitric Oxide in Ethylene- Induced Stomatal Closure in Vicia faba L.

LIU Jing, HOU Zhi-hui, LIU Guo-hua, HOU Li-xia, LIU Xin

Key Laboratory of Plant Biotechnology in Universities of Shandong Province/College of Life Science, Qingdao Agricultural University,Qingdao 266109, P.R.China

收稿日期:2011-10-21 出版日期:2012-10-01 发布日期:2012-11-12
通讯作者: Correspondence LIU Xin, Tel: +86-532-88030224, E-mail: liuxin6080@yahoo.com.cn
基金资助:
This study was supported by the National Natural Science Foundation of China (30970288 and 31170237), the National Science Foundation of Shandong Province, China (ZR2010CM024), and the Foundation of State Key Laboratory of Plant Physiology and Biochemistry, China (SKLPPBKF11001).

Hydrogen Sulfide May Function Downstream of Nitric Oxide in Ethylene- Induced Stomatal Closure in Vicia faba L.

LIU Jing, HOU Zhi-hui, LIU Guo-hua, HOU Li-xia, LIU Xin

Key Laboratory of Plant Biotechnology in Universities of Shandong Province/College of Life Science, Qingdao Agricultural University,Qingdao 266109, P.R.China

Received:2011-10-21 Online:2012-10-01 Published:2012-11-12
Contact: Correspondence LIU Xin, Tel: +86-532-88030224, E-mail: liuxin6080@yahoo.com.cn
Supported by:
This study was supported by the National Natural Science Foundation of China (30970288 and 31170237), the National Science Foundation of Shandong Province, China (ZR2010CM024), and the Foundation of State Key Laboratory of Plant Physiology and Biochemistry, China (SKLPPBKF11001).

摘要/Abstract

摘要： Pharmacological, laser scanning confocal microscopic (LSCM), and spectrophotographic approaches were used to study the roles of hydrogen sulfide (H2S) and nitric oxide (NO) in signaling transduction of stomatal movement in response to ethylene in Vicia faba L. Ethylene treatment resulted in the dose-dependent stomatal closure under light, and this effect was blocked by the inhibitors of H2S biosynthesis in V. faba L. Additionally, ethylene induces H2S generation and increases L-/D-cysteine desulfhydrase (pyridoxalphosphate-dependent enzyme) activity in leaves of V. faba L. Inhibitors of H2S biosynthesis have no effect on the ethylene-induced stomatal closure, NO accumulation, and nitrate reductase (NR) activity in guard cells or leaves of V. faba L. Moreover, the ethylene-induced increase of H2S levels and L-/Dcysteine desulfhydrase activity declined when NO generation was inhibited. Therefore, we conclude that H2S and NO probably are involved in the signal transduction pathway of ethylene-induced stomatal closure. H2S may represent a novel component downstream of NO in the ethylene-induced stomatal movement in V. faba L.

关键词: hydrogen sulfide , nitric oxide , ethylene , stomatal closure , Vicia faba L.

Abstract: Pharmacological, laser scanning confocal microscopic (LSCM), and spectrophotographic approaches were used to study the roles of hydrogen sulfide (H2S) and nitric oxide (NO) in signaling transduction of stomatal movement in response to ethylene in Vicia faba L. Ethylene treatment resulted in the dose-dependent stomatal closure under light, and this effect was blocked by the inhibitors of H2S biosynthesis in V. faba L. Additionally, ethylene induces H2S generation and increases L-/D-cysteine desulfhydrase (pyridoxalphosphate-dependent enzyme) activity in leaves of V. faba L. Inhibitors of H2S biosynthesis have no effect on the ethylene-induced stomatal closure, NO accumulation, and nitrate reductase (NR) activity in guard cells or leaves of V. faba L. Moreover, the ethylene-induced increase of H2S levels and L-/Dcysteine desulfhydrase activity declined when NO generation was inhibited. Therefore, we conclude that H2S and NO probably are involved in the signal transduction pathway of ethylene-induced stomatal closure. H2S may represent a novel component downstream of NO in the ethylene-induced stomatal movement in V. faba L.

Key words: hydrogen sulfide , nitric oxide , ethylene , stomatal closure , Vicia faba L.

LIU Jing, HOU Zhi-hui, LIU Guo-hua, HOU Li-xia, LIU Xin. Hydrogen Sulfide May Function Downstream of Nitric Oxide in Ethylene- Induced Stomatal Closure in Vicia faba L.[J]. Journal of Integrative Agriculture, 2012, 12(10): 1644-1653.

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Hydrogen Sulfide May Function Downstream of Nitric Oxide in Ethylene- Induced Stomatal Closure in Vicia faba L.

Hydrogen Sulfide May Function Downstream of Nitric Oxide in Ethylene- Induced Stomatal Closure in Vicia faba L.

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