Journal of Integrative Agriculture ›› 2015, Vol. 14 ›› Issue (12): 2565-2573.DOI: 10.1016/S2095-3119(15)61040-1

• 论文 • 上一篇    下一篇

Transgenic Arabidopsis thaliana expressing a wheat oxalate oxidase exhibits hydrogen peroxide related defense response

 WEI Fang, HU Jie, YANG Yan, HAO Zhi-da, WU Rui-hua, TIAN Bao-ming, CAO Gang-qiang, ZANG Xin   

  1. 1、School of Life Sciences, Zhengzhou University, Zhengzhou 450001, P.R.China
    2、Zhengzhou Key Laboratory of Plant Molecular Breeding, Zhengzhou 450001, P.R.China
    3、Henan Key Laboratory of Bioactive Macromolecules, Zhengzhou 450001, P.R.China
  • 收稿日期:2014-11-18 出版日期:2015-12-01 发布日期:2015-12-16
  • 通讯作者: TIAN Bao-ming, Tel/Fax: +86-371-67739513, E-mail: tianbm@zzu.edu.cn
  • 作者简介:WEI Fang, Tel/Fax: +86-371-67739513, E-mail: fangwei@zzu.edu.cn;* These authors contributed equally to this study.
  • 基金资助:

    This work was financially supported by the National Key Technology R&D Program of China (2010BAD01B02) and the National Natural Science Foundation of China (U1204308), and the Education Department of Henan Province, China (13A180437).

Transgenic Arabidopsis thaliana expressing a wheat oxalate oxidase exhibits hydrogen peroxide related defense response

 WEI Fang, HU Jie, YANG Yan, HAO Zhi-da, WU Rui-hua, TIAN Bao-ming, CAO Gang-qiang, ZANG Xin   

  1. 1、School of Life Sciences, Zhengzhou University, Zhengzhou 450001, P.R.China
    2、Zhengzhou Key Laboratory of Plant Molecular Breeding, Zhengzhou 450001, P.R.China
    3、Henan Key Laboratory of Bioactive Macromolecules, Zhengzhou 450001, P.R.China
  • Received:2014-11-18 Online:2015-12-01 Published:2015-12-16
  • Contact: TIAN Bao-ming, Tel/Fax: +86-371-67739513, E-mail: tianbm@zzu.edu.cn
  • About author:WEI Fang, Tel/Fax: +86-371-67739513, E-mail: fangwei@zzu.edu.cn;* These authors contributed equally to this study.
  • Supported by:

    This work was financially supported by the National Key Technology R&D Program of China (2010BAD01B02) and the National Natural Science Foundation of China (U1204308), and the Education Department of Henan Province, China (13A180437).

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摘要： Oxalic acid (OA) is considered as an important pathogenetic factor of some destructive diseases caused by some fungal pathogens such as Sclerotinia sclerotiorum. Oxalate degradation is important for plant health, and plants that contain oxalate oxidase (OXO) enzymes could breakdown oxalate into CO2 and H2O2, which subsequently evokes defense responses. However, some species, such as Arabidopsis thaliana, have no oxalate oxidase activity identified to date. The present study aims to develop transgenic Arabidopsis expressing a wheat oxalate oxidase, to test for the response to OA exposure and fungal infection by S. sclerotiorum. The results showed that the transgenic Arabidopsis lines that expressed the wheat OXO exhibited enhanced resistance to OA exposure and S. sclerotiorum infection in the tolerance assays. In the same manner, it could convert OA to CO2 and H2O2 to a higher extent than the wild-type. Intensive osmotic adjustments were also detected in the transgenic Arabidopsis lines. The higher level of produced H2O2 subsequently induced an elevated activity of antioxidant enzymes including superoxide dismutase (SOD) and peroxidase (POD) in the transgenic Arabidopsis plants. The present study indicated that the expression of a gene encoding wheat OXO could induce intensive osmotic adjustments and hydrogen peroxide related defense response, and subsequently increased tolerance to S. sclerotiorum in transgenic A. thaliana.

关键词: oxalate oxidase , oxalic acid , Sclerotinia sclerotiorum , hydrogen peroxide , Arabidopsis thaliana

Abstract: Oxalic acid (OA) is considered as an important pathogenetic factor of some destructive diseases caused by some fungal pathogens such as Sclerotinia sclerotiorum. Oxalate degradation is important for plant health, and plants that contain oxalate oxidase (OXO) enzymes could breakdown oxalate into CO2 and H2O2, which subsequently evokes defense responses. However, some species, such as Arabidopsis thaliana, have no oxalate oxidase activity identified to date. The present study aims to develop transgenic Arabidopsis expressing a wheat oxalate oxidase, to test for the response to OA exposure and fungal infection by S. sclerotiorum. The results showed that the transgenic Arabidopsis lines that expressed the wheat OXO exhibited enhanced resistance to OA exposure and S. sclerotiorum infection in the tolerance assays. In the same manner, it could convert OA to CO2 and H2O2 to a higher extent than the wild-type. Intensive osmotic adjustments were also detected in the transgenic Arabidopsis lines. The higher level of produced H2O2 subsequently induced an elevated activity of antioxidant enzymes including superoxide dismutase (SOD) and peroxidase (POD) in the transgenic Arabidopsis plants. The present study indicated that the expression of a gene encoding wheat OXO could induce intensive osmotic adjustments and hydrogen peroxide related defense response, and subsequently increased tolerance to S. sclerotiorum in transgenic A. thaliana.

Key words: oxalate oxidase , oxalic acid , Sclerotinia sclerotiorum , hydrogen peroxide , Arabidopsis thaliana