Induction of Hypersensitive Response and Nonhost Resistance by a Cladosporium fulvum Elicitor CfHNNI1 is Dose-Dependent and Negatively Regulated by Salicylic Acid

doi:10.1016/S1671-2927(00)8699

Journal of Integrative Agriculture ›› 2012, Vol. 12 ›› Issue (10): 1665-1674.DOI: 10.1016/S1671-2927(00)8699

Induction of Hypersensitive Response and Nonhost Resistance by a Cladosporium fulvum Elicitor CfHNNI1 is Dose-Dependent and Negatively Regulated by Salicylic Acid

XU You-ping, CHEN Hui-ying, ZHOU Xin, CAI Xin-zhong

1.Institute of Biotechnology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, P.R.China
2.Center of Analysis and Measurement, Zhejiang University, Hangzhou 310058, P.R.China
3.Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture/Zhejiang University, Hangzhou 310058,P.R.China

收稿日期:2011-11-11 出版日期:2012-10-01 发布日期:2012-11-12
通讯作者: Correspondence CAI Xin-zhong, Tel: +86-571-88982936, Fax: +86-571-88982936, E-mail: xzhcai@zju.edu.cn
作者简介:XU You-ping, E-mail: ypxu@zju.edu.cn
基金资助:
This work was financially supported by grants from the National Basic Research Program of China (2009CB119000), the Genetically Modified Organisms Breeding Major Projects (2009ZX08009-044B), the PCSIRT Project (IRT0943), the Fundamental Research Funds for the Central Universities (2011XZZX006), and the Program for New Century 151 Talents of Zhejiang Province, China.

Induction of Hypersensitive Response and Nonhost Resistance by a Cladosporium fulvum Elicitor CfHNNI1 is Dose-Dependent and Negatively Regulated by Salicylic Acid

XU You-ping, CHEN Hui-ying, ZHOU Xin, CAI Xin-zhong

1.Institute of Biotechnology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, P.R.China
2.Center of Analysis and Measurement, Zhejiang University, Hangzhou 310058, P.R.China
3.Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture/Zhejiang University, Hangzhou 310058,P.R.China

Received:2011-11-11 Online:2012-10-01 Published:2012-11-12
Contact: Correspondence CAI Xin-zhong, Tel: +86-571-88982936, Fax: +86-571-88982936, E-mail: xzhcai@zju.edu.cn
About author:XU You-ping, E-mail: ypxu@zju.edu.cn
Supported by:
This work was financially supported by grants from the National Basic Research Program of China (2009CB119000), the Genetically Modified Organisms Breeding Major Projects (2009ZX08009-044B), the PCSIRT Project (IRT0943), the Fundamental Research Funds for the Central Universities (2011XZZX006), and the Program for New Century 151 Talents of Zhejiang Province, China.

摘要/Abstract

摘要： Nonhost resistance is a phenomenon that enables plants to protect themselves against the majority of potential pathogens, and thus has a great potential for application in plant protection. We recently found that CfHNNI1 (for Cladosporium fulvum host and nonhost plant necrosis inducer 1) is an inducer of plant hypersensitive response (HR) and nonhost resistance. In this study, its functional mechanism was analyzed. CfHNNI1 was a single copy gene in C. fulvum genome. The functional ORF of the CfHNNI1 cDNA was ATG3-TAG780, which showed homology with genes encoding bZIP transcription factors. The functional ORF included in frame an inner one ATG273-TAG780, which was sufficient to induce HR in plants. CfHNNI1 induced plant HR in a dose-dependent manner. CfHNNI1-induced necrosis in NahG transgenic tomato plants was significantly stronger than that in their wild type controls. However, the necrosis in Nr and def1 tomato mutants was similar to that in their corresponding wild type plants. These data demonstrate that induction of HR and nonhost resistance by CfHNNI1 is negatively regulated by salicylic acid signalling pathway but independent of ethylene and jasmonic acid signalling pathways.

关键词: CfHNNI1 , Cladosporium fulvum , ethylene , hypersensitive response , jasmonic acid , nonhost resistance , salicylic acid

Abstract: Nonhost resistance is a phenomenon that enables plants to protect themselves against the majority of potential pathogens, and thus has a great potential for application in plant protection. We recently found that CfHNNI1 (for Cladosporium fulvum host and nonhost plant necrosis inducer 1) is an inducer of plant hypersensitive response (HR) and nonhost resistance. In this study, its functional mechanism was analyzed. CfHNNI1 was a single copy gene in C. fulvum genome. The functional ORF of the CfHNNI1 cDNA was ATG3-TAG780, which showed homology with genes encoding bZIP transcription factors. The functional ORF included in frame an inner one ATG273-TAG780, which was sufficient to induce HR in plants. CfHNNI1 induced plant HR in a dose-dependent manner. CfHNNI1-induced necrosis in NahG transgenic tomato plants was significantly stronger than that in their wild type controls. However, the necrosis in Nr and def1 tomato mutants was similar to that in their corresponding wild type plants. These data demonstrate that induction of HR and nonhost resistance by CfHNNI1 is negatively regulated by salicylic acid signalling pathway but independent of ethylene and jasmonic acid signalling pathways.

Key words: CfHNNI1 , Cladosporium fulvum , ethylene , hypersensitive response , jasmonic acid , nonhost resistance , salicylic acid

XU You-ping, CHEN Hui-ying, ZHOU Xin, CAI Xin-zhong. Induction of Hypersensitive Response and Nonhost Resistance by a Cladosporium fulvum Elicitor CfHNNI1 is Dose-Dependent and Negatively Regulated by Salicylic Acid[J]. Journal of Integrative Agriculture, 2012, 12(10): 1665-1674.

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Induction of Hypersensitive Response and Nonhost Resistance by a Cladosporium fulvum Elicitor CfHNNI1 is Dose-Dependent and Negatively Regulated by Salicylic Acid

Induction of Hypersensitive Response and Nonhost Resistance by a Cladosporium fulvum Elicitor CfHNNI1 is Dose-Dependent and Negatively Regulated by Salicylic Acid

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