Effects of Nitric Oxide on the Quality and Pectin Metabolism of Yali Pears During Cold Storage

doi:10.1016/S1671-2927(11)60102-8

摘要/Abstract

摘要： The effect of fumigation with 10, 20, and 30 μL L-1 nitric oxide (NO) was investigated to study the effects of NO on thequality of Yali pears during cold storage. The ethylene production, composition of cell walls, and cell-wall-modifyingenzyme activities were measured on fruits which were fumigated with NO (20 μL L-1). The results showed that NO not onlyreduced the peak value of ethylene production rate, the soluble sugar, soluble solid content, maintained higher firmness,starch, and NO content, but also retarded the degradation of covalent soluble pectin, accumulation of ionic soluble pectinand water soluble pectin. Moreover, NO fumigation decreased the activities of polygalacturonase (PG) and â-galactosidase(â-Gal) and delayed the peak of PG activity of fruits. Therefore, it indicated that NO fumigation could delay the softeningand ripening of Yali pears.

关键词:

pear, nitric oxide, cell wall material, fruit softening

Abstract: The effect of fumigation with 10, 20, and 30 μL L-1 nitric oxide (NO) was investigated to study the effects of NO on thequality of Yali pears during cold storage. The ethylene production, composition of cell walls, and cell-wall-modifyingenzyme activities were measured on fruits which were fumigated with NO (20 μL L-1). The results showed that NO not onlyreduced the peak value of ethylene production rate, the soluble sugar, soluble solid content, maintained higher firmness,starch, and NO content, but also retarded the degradation of covalent soluble pectin, accumulation of ionic soluble pectinand water soluble pectin. Moreover, NO fumigation decreased the activities of polygalacturonase (PG) and â-galactosidase(â-Gal) and delayed the peak of PG activity of fruits. Therefore, it indicated that NO fumigation could delay the softeningand ripening of Yali pears.

Key words:

pear, nitric oxide, cell wall material, fruit softening

LIU Li-qin, DONG Yu, GUAN Jun-feng. Effects of Nitric Oxide on the Quality and Pectin Metabolism of Yali Pears During Cold Storage[J]. Journal of Integrative Agriculture, 2011, 10(7): 1125-1133.

参考文献

[1]       Andrews P K, Li S L. 1995. Cell wall hydrolytic enzyme activityduring development of nonclimacteric sweet cherry (Prunusavium L.) fruit. Journal of Horticultural Science, 70, 561-567.
[2]       Blumenkrantz N, Asboe-Hansen G. 1973. New method forquantitative determination of uronic acids. AnalyticalBiochemistry, 54, 484-489.
[3]       Bradford M M. 1976. A rapid and sensitive method forquantitation of microgram quantities of protein utilizing theprinciple of protein-dye binding. Analytical Biochemistry,72, 248-254.
[4]       Brummell D A, Cin V D, Crisosto C H, Labavitch J M. 2004.Cell wall metabolism during maturation,ripening andsenescence of peach fruit. Journal of Experimental Botany,55, 2029-2039.
[5]       Carrington C M S, Creve L C, Lahavitch J M. 1993. Cell wallmetabolism in ripening fruit. VI. Effect of the antisensepolygalacturonase gene on cell wall changes accompanyingripening in transgenic tornatoes. Plant Physiology, 103, 429-434.
[6]       Cheng G P, Yang E, Lu W J. 2009. Effect of nitric oxide onethylene synthesis snd softening of banana fruit slice duringripening. Journal of Agricultural and Food Chemistry, 57,5799-5804.
[7]       Ciobotari G, Morariu A, Gradinariu G. 2009. Aspects of graftinginfluence on carbon and nitrogen movement of several pear(Pyrus sativa) cultivars. Notulae Boanicaet Horti AgrobotaniciCluj-Napoca, 37, 134-138.
[8]       Fairbairn N J. 1953. A modified anthrone reagent. ChemicalIndustries, 4, 86.Fernandez-Trujillo J P, Cano A, Artés F. 2000. Interactions amongcooling, fungicide and postharvest ripening temperature onpeaches. International Journal of Refrigeration, 30, 441-450.
[9]       Fischer R L, Bennett A B. 1991. Role of cell wall hydrolases infruit ripening. Annual Review of Plant Physiology and PlantMolecular Biology, 42, 675-703.
[10]    Fishman M L, Levaj B, Gillespie D, Scorza R. 1993. Changes inthe physico-chemical properties of peach fruit pectin duringon-tree ripening and storage. Journal of the American Societyfor Horticultural Science, 118, 343-349.
[11]    Francisco B. 2008. Effect of a pretreatment with nitric oxide onpeach (Prunus persica L.) storage at room temperature.European Food Research and Technology, 227, 1599-1611.
[12]    Gross K C. 1982. A rapid and sensitive spectrophotometricmethod for assaying polygalacturonase using 2-cyanoacetamide. Horticultural Science, 17, 933-934.
[13]    Hu X, Fang J, Cai W, Tang Z. 2003. NO-mediated hypersensitiveresponses of rice suspension cultures induced by incompatibleelicitor. Chinese Science Bulletin, 48, 358-363.
[14]    Huber D J. 1984. Strawberry fruit softening: the potential rolesof polyuronides and hemicelluloses. Journal of Food Science,49, 1310-1315.
[15]    Leshem Y Y, Pinchasov Y. 2000. Non-invasive photoacousticspectroscopic determination of relative endogenous nitricoxide and ethylene content stoichiometry during the ripeningof strawberries Fragaria anannasa (Duch.) and avocadosPersea americana (Mill.). Journal Experimental Botany, 51,1471-1473.
[16]    Leshem Y Y, Wills R B H. 1998. Harnessing senescence delayinggeses nitric oxide and nitrous oxide a novel approach topostharvest control of fresh horticultural produce. Biologyof Plant, 41, 1-10.
[17]    Leshem Y Y, Wills R B H, Ku V V V. 1998. Evidence for thefunction of the free radical gas - nitric oxide (NO) - as anendogenous maturation and senescence regulating factor inhigher plants. Plant Physiology and Biochemistry, 36, 825-833.
[18]    Leshem Y Y, Wills R B H, Yehoushua B S. 2001. Application ofnitric oxide (NO) for postharvest control. Acta HorticultureScience, 553, 571-575.
[19]    Radi R, Beckman J S, Bush K M, Freeman B A. 1991.Peroxynitrite induced membrane lipid per oxidation: thecytotoxic potential of superoxide and nitric oxide. Archivesof Biochemistry and Biophysics, 288, 481-487.
[20]    Ross G S, Redgwell R J, Macrae E A. 1993. Kiwifruit β- galactosidase: isolation and activity against specific fruit cellwallpolysaccharedes. Planta, 189, 499-506.
[21]    Sozzi G O, Trinchero G F, Fraschina A A. 2003. Delayed ripeningof ‘Bartlet’pears treated with nitric oxide. Journal ofHorticultural Science and Biotechnology, 78, 899-903.
[22]    Wang F, Sanz A, Brenner M L, Smith A. 1993. Sucrose synthase,starch accumulation, and tomato fruit sink strength. PlantPhysiology, 101, 321-327.
[23]    Wei J M, Ma F W, Guan J F, Yuan J W, Zhu X Q. 2009. Cell wallmetabolism and its regulation in harvested Pyrus ussuriensisMaxin. cv. Jingbaili fruit during ripening. Scientia AgriculturaSinica, 42, 2987-2996.
[24]    (in Chinese)Wendehenne D, Durner J, Klessing D F. 2004. Nitric oxide: anew player in plant signaling and defence response. CurrentCpinion in Plant Biology, 7, 449-455.
[25]    Zhou H W, Sonego L, Ben-Arie R, Lurie S. 1999. Analysis of cellwall components in juice of ‘Flavortop’ nectarines duringnormal ripening and woolliness development. Journal of theAmerican Society for Horticultural Science, 124, 424-429.
[26]    Zhu S H, Liu M C, Zhou J. 2006a. Effects of fumigation withnitric oxide on cell wall metabolisms of postharvest feichengpeaches. Scientia Agricultura Sinica, 39, 1878-1884. (inChinese)
[27]   Zhu S H, Liu M C, Zhou J. 2006b. Inhibition by nitric oxide ofethylene biosynthesis and lipoxygenase activity in peachfruit during storage. Postharvest Biology and Technology,42, 41-48.