Effect of Nitric Oxide on Alleviating Cadmium Toxicity in Rice (Oryza sativa L.)

doi:10.1016/S2095-3119(13)60417-7

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (9): 1540-1550.DOI: 10.1016/S2095-3119(13)60417-7

Effect of Nitric Oxide on Alleviating Cadmium Toxicity in Rice (Oryza sativa L.)

ZHAO Xiu-feng, CHEN Lin, Muhammad I A Rehmani, WANG Qiang-sheng, WANG Shao-hua, HOU Pengfu, LI Gang-hua , DING Yan-feng

College of Agriculture, Nanjing Agricultural University/Key Laboratory of Crop Physiology and Ecology in Southern China, Ministry of Agriculture, Nanjing 210095, P.R.China

收稿日期:2012-08-19 出版日期:2013-09-01 发布日期:2013-09-15
通讯作者: Correspondence DING Yan-feng, Tel/Fax: +86-25-84395066, E-mail, dingyf@njau.edu.cn
基金资助:
This work was supported by the Key Technologies R&D Program of China during the 11th Five-Year Plan period (2006BAD02A03), the the Foundation of Key Program of Jiangsu Province (2007JA148) and the Natural Science Foundation of Jiangsu Province, China (BK2010449).

Effect of Nitric Oxide on Alleviating Cadmium Toxicity in Rice (Oryza sativa L.)

ZHAO Xiu-feng, CHEN Lin, Muhammad I A Rehmani, WANG Qiang-sheng, WANG Shao-hua, HOU Pengfu, LI Gang-hua , DING Yan-feng

College of Agriculture, Nanjing Agricultural University/Key Laboratory of Crop Physiology and Ecology in Southern China, Ministry of Agriculture, Nanjing 210095, P.R.China

Received:2012-08-19 Online:2013-09-01 Published:2013-09-15
Contact: Correspondence DING Yan-feng, Tel/Fax: +86-25-84395066, E-mail, dingyf@njau.edu.cn
Supported by:
This work was supported by the Key Technologies R&D Program of China during the 11th Five-Year Plan period (2006BAD02A03), the the Foundation of Key Program of Jiangsu Province (2007JA148) and the Natural Science Foundation of Jiangsu Province, China (BK2010449).

摘要/Abstract

摘要： Nitric oxide (NO) is a gaseous signaling molecule in plants that plays a key role in mediating a wide range of physiological processes and responses to biotic and abiotic stresses. The present study was conducted to investigate the effects of the exogenous application of sodium nitroprusside (SNP), an NO donor, on cadmium (Cd)-induced oxidative stress and Cd uptake in rice plants. Rice plants were exposed to Cd stress (0.2 mmol L-1 CdCl2) and different concentrations of SNP (0.05, 0.1, 0.2, and 0.4 mmol L-1). A SNP concentration of 0.1 mmol L-1 (SNP10) significantly reduced the Cd-induced decrease in shoot and root dry weights and leaf chlorophyll concentrations. The addition of NO also reduced the malondialdehyde (MDA), hydrogen peroxide (H2O2) and ascorbic acid (ASA) concentrations. However, the reduction in glutathione (GSH) concentration was inhibited by NO treatment. Moreover, NO prevented the Cd-induced increase in antioxidative enzyme activity. The amount of Cd accumulation in rice plants was also influenced by the addition of NO. The NO supplied by the SNP enhanced the Cd tolerance of the rice by increasing the Cd uptake by the roots and decreasing the Cd accumulation by the shoots. However, the application of potassium ferrocyanide (Cd+Fe) or sodium nitrate and nitrite (Cd+N) (without NO release), did not exhibit the effects of the SNP. Furthermore, the effects of the SNP were reversed by the addition of hemoglobin (an NO scavenger). Our results suggested that exogenous NO was involved in the resistance of rice to Cdtoxicity.

关键词: cadmium toxicity , nitric oxide , oxidative stress , Oryza sativa L.

Abstract: Nitric oxide (NO) is a gaseous signaling molecule in plants that plays a key role in mediating a wide range of physiological processes and responses to biotic and abiotic stresses. The present study was conducted to investigate the effects of the exogenous application of sodium nitroprusside (SNP), an NO donor, on cadmium (Cd)-induced oxidative stress and Cd uptake in rice plants. Rice plants were exposed to Cd stress (0.2 mmol L-1 CdCl2) and different concentrations of SNP (0.05, 0.1, 0.2, and 0.4 mmol L-1). A SNP concentration of 0.1 mmol L-1 (SNP10) significantly reduced the Cd-induced decrease in shoot and root dry weights and leaf chlorophyll concentrations. The addition of NO also reduced the malondialdehyde (MDA), hydrogen peroxide (H2O2) and ascorbic acid (ASA) concentrations. However, the reduction in glutathione (GSH) concentration was inhibited by NO treatment. Moreover, NO prevented the Cd-induced increase in antioxidative enzyme activity. The amount of Cd accumulation in rice plants was also influenced by the addition of NO. The NO supplied by the SNP enhanced the Cd tolerance of the rice by increasing the Cd uptake by the roots and decreasing the Cd accumulation by the shoots. However, the application of potassium ferrocyanide (Cd+Fe) or sodium nitrate and nitrite (Cd+N) (without NO release), did not exhibit the effects of the SNP. Furthermore, the effects of the SNP were reversed by the addition of hemoglobin (an NO scavenger). Our results suggested that exogenous NO was involved in the resistance of rice to Cdtoxicity.

Key words: cadmium toxicity , nitric oxide , oxidative stress , Oryza sativa L.

ZHAO Xiu-feng, CHEN Lin, Muhammad I A Rehmani, WANG Qiang-sheng, WANG Shao-hua, HOU Pengfu, LI Gang-hua , DING Yan-feng. Effect of Nitric Oxide on Alleviating Cadmium Toxicity in Rice (Oryza sativa L.)[J]. Journal of Integrative Agriculture, 2013, 12(9): 1540-1550.

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Effect of Nitric Oxide on Alleviating Cadmium Toxicity in Rice (Oryza sativa L.)

Effect of Nitric Oxide on Alleviating Cadmium Toxicity in Rice (Oryza sativa L.)

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