关键词: 白菜, cDNA-AFLP, 转录图谱

Abstract: 【Objective】Developing construction of cDNA-AFLPs molecular transcriptome map of Chinese cabbage-pak-choi (Brassica campestris L. ssp. chinensis Makino), which can establish the molecular basis of molecular breeding and heterosis for Chinese cabbage.【Method】With the inbred line ‘Aijiaohuang 97-3-2’, the inbred line ‘Baimanjing 001-24’ of turnip [B. campestris ssp. rapifera (Matzg.) Sinsk ] and F6 recombinant inbred(RI) population as experimental materials, Using 56 couples cDNA-AFLP primers out of 256, the segregation and distribution of cDNA-AFLPs in parents and F6 plants were analysized. 【Result】A total of 138 cDNA-AFLPs marker loci were mapped to 12 linkage groups, covering 1309.5 cM with an average distance of 9.2 cM. 【Conclusion】It was the first transcriptome map of Chinese cabbage by using cDNA-AFLP.