稳定同位素技术在肉羊产地溯源中的应用

doi:10.3864/j.issn.0578-1752.2021.02.014

中国农业科学 ›› 2021, Vol. 54 ›› Issue (2): 392-399.doi: 10.3864/j.issn.0578-1752.2021.02.014

稳定同位素技术在肉羊产地溯源中的应用

王倩^1,²(),李政^1,³(),赵姗姗¹,郄梦洁¹,张九凯⁴,王明林³,郭军²,赵燕¹()

¹中国农业科学院农业质量标准与检测技术研究所/农业农村部农产品质量安全重点实验室,北京 100081
²内蒙古农业大学食品科学与工程学院,呼和浩特 010018
³山东农业大学食品科学与工程学院,山东泰安 271018
⁴中国检验检疫科学研究院,农产品安全研究中心,北京 100176

收稿日期:2020-05-21 接受日期:2020-09-03 出版日期:2021-01-16 发布日期:2021-02-03
通讯作者: 赵燕
作者简介:王倩,Tel：15049144206;E-mail： 1639828031@qq.com。|李政,Tel：15652057927;E-mail： 408209783@qq.com。
基金资助:
国家“十三五”重点研发计划(2017YFC1601703)

Application of Stable Isotope Technology in the Origin Traceability of Sheep

WANG Qian^1,²(),LI Zheng^1,³(),ZHAO ShanShan¹,QIE MengJie¹,ZHANG JiuKai⁴,WANG MingLin³,GUO Jun²,ZHAO Yan¹()

¹Institute of Agricultural Quality Standards & Testing Technology, Chinese Academy of Agricultural Sciences, Key Laboratory of Agricultural Product Quality and Safety, Ministry of Agriculture and Rural Areas, Beijing 100081
²College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018
³College of Food Science and Engineering, Shandong Agricultural University, Taian 271018
⁴Chinese Academy of Inspection and Quarantine, Agro-Product Safety Research Center, Beijing 100176

Received:2020-05-21 Accepted:2020-09-03 Online:2021-01-16 Published:2021-02-03
Contact: Yan ZHAO

摘要/Abstract

摘要：

【目的】通过比较不同部位羊肉中碳氮稳定同位素差异,以及对羊肉、全骨粉、脱脂骨粉与骨胶原的稳定同位素分析,为肉羊产地溯源鉴别提供技术支持。【方法】利用同位素比率质谱仪（IRMS）测定不同部位脱脂羊肉中δ¹³C和δ¹⁵N值,进而比较不同产地来源（新西兰和中国宁夏、甘肃、安徽）的羊肉、全骨粉、脱脂骨粉与骨胶原中δ¹³C和δ¹⁵N值,并对结果进行方差分析、线性判别分析与相关性分析,确定碳氮稳定同位素对肉羊产地的溯源能力。【结果】后腿、排骨与胸叉、腹腩、脖子中δ¹³C值具有显著性差异（P<0.05）,δ¹⁵N值在后腿、胸叉、排骨、腹腩和脖子5个部位间无显著差异（P>0.05）。不同产地（宁夏、甘肃、安徽和新西兰）的脱脂羊肉、全骨粉、脱脂骨粉与骨胶原中δ¹³C和δ¹⁵N值差异显著（P<0.05）,其中脱脂羊肉对产地溯源判别效果最佳,原始判别正确率为84.9%,交叉验证判别正确率为82.4%。全骨粉与骨胶原对产地判别正确率达65%以上。相关性分析结果表明脱脂羊肉、全骨粉、脱脂骨粉与骨胶原中δ¹³C、δ¹⁵N具有极显著相关性（P<0.01）,骨胶原与脱脂骨粉、全骨粉中碳同位素相关性最高,相关系数分别为0.903和0.866。【结论】稳定同位素可有效鉴别不同产地来源的肉羊样品,羊肉、全骨粉、脱脂骨粉及骨胶原中稳定同位素组成变化趋势基本一致,因此,利用稳定同位素技术对肉羊进行产地溯源是可行的。

关键词: 稳定同位素, 羊肉, 羊骨, 产地溯源

Abstract:

【Objective】By comparing the differences of carbon and nitrogen stable isotopes in different parts of mutton and analyzing the stable isotopes of mutton, whole bone meal, de-fatted bone meal and bone collagen, the technical support was provided for the origin traceability identification of sheep. 【Method】Isotope Ratio Mass Spectrometer (IRMS) was used to determine δ ¹³C and δ ¹⁵N values in different parts of sheep, and δ ¹³C and δ ¹⁵N values in de-fatted mutton, whole bone meal, de-fatted bone meal and bone collagen of the sheep bones from different origins (New Zealand, and Ningxia, Gansu, Anhui, China). ANOVA test, linear discriminant analysis and correlation analysis were carried out on the results to determine the traceability of carbon and nitrogen stable isotope to the origin of sheep. 【Result】The δ ¹³C values of hind legs and ribs were significantly different from those of chest fork, abdomen and neck (P<0.05), and there was no significant difference in δ¹⁵N values among the five parts of hind legs, chest fork, ribs, abdomen and neck (P>0.05). The δ¹³C and δ ¹⁵N values of de-fatted mutton, whole bone meal, de-fatted bone meal and bone collagen from different origins (Ningxia, Gansu, Anhui and New Zealand) were significantly different (P<0.05). De-fatted mutton had the best effect on the origin traceability, with the original discrimination accuracy rate was 84.9% and the cross-validation discrimination accuracy rate was 82.4%. The accuracy rate of distinguishing the origin by whole bone meal and bone collagen was over 65%. The correlation analysis results show that de-fatted mutton, whole bone meal, de-fatted bone meal and bone collagen had extremely significant correlation (P<0.01), and the carbon isotope in bone collagen had the highest correlation with de-fatted bone meal and whole bone meal, with correlation coefficients of 0.903 and 0.866, respectively. 【Conclusion】Stable isotope could effectively identify sheep samples from different origins, and the change trend of stable isotope composition in mutton, whole bone meal, de-fatted bone meal and bone collagen was basically the same, so it was feasible to trace the origin of sheep by using stable isotope technology.

Key words: stable isotope, mutton, sheep bone, origin traceability

王倩,李政,赵姗姗,郄梦洁,张九凯,王明林,郭军,赵燕. 稳定同位素技术在肉羊产地溯源中的应用[J]. 中国农业科学, 2021, 54(2): 392-399.

WANG Qian,LI Zheng,ZHAO ShanShan,QIE MengJie,ZHANG JiuKai,WANG MingLin,GUO Jun,ZHAO Yan. Application of Stable Isotope Technology in the Origin Traceability of Sheep[J]. Scientia Agricultura Sinica, 2021, 54(2): 392-399.

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https://www.chinaagrisci.com/CN/Y2021/V54/I2/392

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参考文献 33

[1]	MAI Z H, LAI B, SUN M W, SHAO J L, GUO L X. Food adulteration and traceability tests using stable carbon isotope technologies. Tropical Journal of Pharmaceutical Research, 2019,18(8):1771-1784.
[2]	VIOLINO S, ANTONUCCI F, PALLOTTINO F, CECCHINI C, FIGORILLI S, COSTA C. Food traceability: A term map analysis basic review. European Food Research and Technology, 2019,245(10):2089-2099.
[3]	BONER M, FORSTEL H. Stable isotope variation as a tool to trace the authenticity of beef. Analytical and Bioanalytical Chemistry, 2004,378(2):301-310.
[4]	FRANKE B M, KOSLITZ S, MICAUX F, PIANTINI U, MAURY V, PFAMMATTER E, WUNDERLI S, GREMAUD G, BOSSET J, HADORN R, KREUZER M. Tracing the geographic origin of poultry meat and dried beef with oxygen and strontium isotope ratios. European Food Research and Technology, 2008,226(4):761-769.
[5]	FOERSTEL H. The natural fingerprint of stable isotopes-use of IRMS to test food authenticity. Analytical and Bioanalytical Chemistry, 2007,388(3):541-544. pmid: 17396248
[6]	LV J, ZHAO Y. Combined stable isotopes and multi-element analysis to research the difference between organic and conventional chicken. Food Analytical Methods, 2017,10(2):347-353.
[7]	ZHAO Y, ZHANG B, CHEN G, CHEN A L, YANG S M, YE Z H. Tracing the geographic origin of beef in china on the basis of the combination of stable isotopes and multielement analysis. Journal of Agricultural and Food Chemistry, 2013,61(29):7055-7060.
[8]	郭波莉, 魏益民, 魏帅, 孙倩倩, 张磊, 师振强. 牦牛肉中稳定同位素指纹特征及影响因素. 中国农业科学, 2018,51(12):2391-2397.
	GUO B L, WEI Y M, WEI S, SUN Q Q, ZHANG L, SHI Z Q. The characters and influence factors of stable isotope fingerprints in yak muscle. Scientia Agricultura Sinica, 2018,51(12):2391-2397. (in Chinese)
[9]	MONAHAN F J, SCHMIDT O, MOLONEY A P. Meat provenance: Authentication of geographical origin and dietary background of meat. Meat Science, 2018,144:2-14.
[10]	BIONDI L, D'URSO M G, VASTA V, LUCIANO G, SCERRA M, PRIOLO A, ZILLER L, BONTEMPO L, CAPARRA P, CAMIN F. Stable isotope ratios of blood components and muscle to trace dietary changes in lambs. Animal, 2013,7(9):1559-1566.
[11]	BONTEMPO L, CAMIN F, ZILLER L, BIONDI L, D'URSO M G, VASTA V, LUCIANO G. Variations in stable isotope ratios in lamb blood fractions following dietary changes: A preliminary study. Rapid Communications in Mass Spectrometry, 2016,30(1):170-174. pmid: 26661984
[12]	ERASMUS S W, MULLER M, BUTLER M, HOFFMAN L C. The truth is in the isotopes: Authenticating regionally unique South African lamb. Food Chemistry, 2018,239:926-934.
[13]	PIASENTIER E, VALUSSO R, CAMIN F, VERSINI G. Stable isotope ratio analysis for authentication of lamb meat. Meat Science, 2003,64(3):239-247.
[14]	VINCI G, PRETI R, TIERI A, VIERI S. Authenticity and quality of animal origin food investigated by stable-isotope ratio analysis. Journal of the Science of Food and Agriculture, 2013,93(3):439-448.
[15]	SUN S M, GUO B L, WEI Y M. Origin assignment by multi-element stable isotopes of lamb tissues. Food Chemistry, 2016,213:675-681.
[16]	GARBOWSKA B, RADZYMINSKA M, JAKUBOWSKA D. Influence of the origin on selected determinants of the quality of pork meat products. Czech Journal of Food Sciences, 2013,31(6):547-552.
[17]	SAEED O A, SAZILI A Q, AKIT H, EBRAHIMI M, ALIMON A R, SAMSUDIN A A. Effects of corn supplementation on meat quality and fatty acid composition of Dorper lambs fed PKC-Urea treated rice straw. BMC Veterinary Research, 2019,15:233.
[18]	刘畅, 罗玉龙, 张亚琨, 李文博, 赵丽华, 苏琳, 靳烨. 苏尼特羊不同部位肌肉抗氧化系统的差异. 中国食品学报, 2020,20(3):291-297.
	LIU C, LUO Y L, ZHANG Y K, LI W B, ZHAO L H, SU L, JIN Y. Differences of muscle antioxidant system in different positions of sunit sheep. Journal of Chinese Institute of Food Science and Technology, 2020,20(3):291-297. (in Chinese)
[19]	韩克光, 甄守艳, 高文伟, 霍乃蕊. 单酶水解羊骨粉效果比较及水解指标相关性分析. 食品科技, 2016,41(1):110-114.
	HAN K G, ZHEN S Y, GAO W W, HUO N R. Comparison of hydrolyzing effect of sheep bone powder by different proteases and correlation analysis for hydrolysis indicators. Food Science and Technology, 2016,41(1):110-114. (in Chinese)
[20]	JIANG D, DU L, GUO Y C, MA J F, LI X Y, HAN L, XU Y S, QIAN Y. Potential use of stable isotope and multi-element analyses for regional geographical traceability of bone raw materials for gelatin production. Food Analytical Methods, 2020,13(3):762-769.
[21]	HARRISON S M, MONAHAN F J, MOLONEY A P, KELLY S D, CUFFE F, HOOGEWERFF J, SCHMIDT O. Intra-muscular and inter-muscular variation in carbon turnover of ovine muscles as recorded by stable isotope ratios. Food Chemistry, 2010,123(2):203-209.
[22]	郭莉, 张寰, 王燕, 高岩, 郑玉山, 张宏博. 基于碳、氮稳定同位素技术的羊肉产地溯源可行性研究. 肉类工业, 2020(2):25-30.
	GUO L, ZHANG H, WANG Y, GAO Y, ZHENG Y S, ZHANG H B. Feasibility study on origin traceability of mutton based on carbon and nitrogen stable isotope technology. Meat Industry, 2020(2):25-30. (in Chinese)
[23]	FRANKE B M, GREMAUD G, HADORN R, KREUZER M. Geographic origin of meat - elements of an analytical approach to its authentication. European Food Research and Technology, 2005,221(3/4):493-503.
[24]	ERASMUS S W, MULLER M, VAN DER RIJST M, HOFFMAN L C. Stable isotope ratio analysis: A potential analytical tool for the authentication of South African lamb meat. Food Chemistry, 2016,192:997-1005. doi: 10.1016/j.foodchem.2015.07.121 pmid: 26304440
[25]	DE SMET S, BALCAEN A, CLAEYS E, BOECKX P, VAN CLEEMPUT O. Stable carbon isotope analysis of different tissues of beef animals in relation to their diet. Rapid Communications in Mass Spectrometry, 2004,18(11):1227-1232.
[26]	SCHWERTL M, AUERSWALD K, SCHAUFELE R, SCHNYDER H. Carbon and nitrogen stable isotope composition of cattle hair: ecological fingerprints of production systems? Agriculture Ecosystems & Environment, 2005,109(1/2):153-165.
[27]	ROGERS K M. Nitrogen isotopes as a screening tool to determine the growing regimen of some organic and nonorganic supermarket produce from New Zealand. Journal of Agricultural and Food Chemistry, 2008,56(11):4078-4083. doi: 10.1021/jf800797w
[28]	MEKKI I, CAMIN F, PERINI M, SMETI S, HAJJI H, MAHOUACHI M, PIASENTIER E, ATTI N. Differentiating the geographical origin of Tunisian indigenous lamb using stable isotope ratio and fatty acid content. Journal of Food Composition and Analysis, 2016,53:40-48. doi: 10.1016/j.jfca.2016.09.002
[29]	DEVINCENZI T, DELFOSSE O, ANDUEZA D, NABINGER C, PRACHE S. Dose-dependent response of nitrogen stable isotope ratio to proportion of legumes in diet to authenticate lamb meat produced from legume-rich diets. Food Chemistry, 2014,152:456-461. doi: 10.1016/j.foodchem.2013.11.164
[30]	CARRIJO A S, PEZZATO A C, DUCATTI C, SARTORI J R, TRINCA L, SILVA E T. Traceability of bovine meat and bone meal in poultry by stable isotope analysis. Brazilian Journal of Poultry Science, 2006,8(1):63-68.
[31]	POUPIN N, MARIOTTI F, HUNEAU J F, HERMIER D, FOUILLET H. Natural isotopic signatures of variations in body nitrogen fluxes: A compartmental model analysis. PLoS Computational Biology, 2014,10(10):e1003865. pmid: 25275306
[32]	STEVENS R E, O'CONNELL T C. Red deer bone and antler collagen are not isotopically equivalent in carbon and nitrogen. Rapid Communications in Mass Spectrometry, 2016,30(17):1969-1984. pmid: 27501431
[33]	JANSEN O E, AARTS G M, DAS K, LEPOINT G, MICHEL L, REIJNDERS P J H. Feeding ecology of harbour porpoises: stable isotope analysis of carbon and nitrogen in muscle and bone. Marine Biology Research, 2012,8(9):829-841. doi: 10.1080/17451000.2012.692164

样品名称 Sample name	胸叉 Chest fork (n=12)	腹腩 Abdomen (n=12)	脖子 Neck (n=11)	后腿 Hind legs (n=12)	排骨 Pork ribs (n=12)
δ¹³C (‰)	-11.97±0.35a	-12.14±0.38a	-12.19±0.40a	-12.60±0.45b	-13.13±0.55c
δ¹⁵N (‰)	3.25±0.43	3.37±0.26	3.15±0.51	3.23±0.43	3.52±0.39

样品名称 Sample name	脱脂羊肉 De-fatted mutton	全骨粉 Whole bone meal	脱脂骨粉 De-fatted bone meal	骨胶原 Bone collagen
中国宁夏 Ningxia, China (n=20)	-13.16±1.17b	-16.09±1.89b	-12.88±1.44b	-13.70±1.94a
中国甘肃 Gansu, China (n=18)	-11.10±0.35a	-16.05±2.39b	-11.59±2.21a	-13.07±2.38a
中国安徽 Anhui, China (n=20)	-18.90±0.69c	-20.59±2.81c	-16.90±1.90c	-17.62±2.03b
新西兰 New Zealand (n=20)	-13.59±0.47b	-14.29±0.69a	-12.56±0.53ab	-12.59±0.52a

样品名称 Sample name	脱脂羊肉 De-fatted mutton	全骨粉 Whole bone meal	脱脂骨粉 De-fatted bone meal	骨胶原 Bone collagen
中国宁夏 Ningxia, China (n=20)	4.44±0.61b	5.31±1.47a	5.04±1.05a	4.42±1.20a
中国甘肃 Gansu, China (n=18)	3.17±0.42c	4.16±1.12b	4.05±0.62b	3.13±0.71b
中国安徽 Anhui, China (n=20)	5.08±0.44a	5.80±1.05a	5.36±0.74a	4.70±0.71a
新西兰 New Zealand (n=20)	5.39±0.41a	5.39±0.82a	5.36±0.74a	4.29±0.56a

样品 Sample	脱脂羊肉 De-fatted mutton	全骨粉 Whole bone meal	脱脂骨粉 De-fatted bone meal	骨胶原 Bone collagen
中国宁夏 Ningxia, China	60.0%	40.0%	40.0%	40.0%
中国甘肃 Gansu, China	90.0%	80.0%	80.0%	85.0%
中国安徽 Anhui, China	95.0%	90.0%	50.0%	75.0%
新西兰 New Zealand	94.4%	72.2%	88.9%	77.8%
原始判别正确率 Original Discriminant Accuracy	84.9%	70.6%	64.7%	69.5%
中国宁夏 Ningxia, China	50.0%	30.0%	35.0%	35.0%
中国甘肃 Gansu, China	90.0%	75.0%	80.0%	80.0%
中国安徽 Anhui, China	95.0%	90.0%	50.0%	75.0%
新西兰 New Zealand	94.4%	72.2%	88.9%	77.8%
交叉验证判别正确率 Cross-validation discrimination accuracy	82.4%	66.8%	63.5%	67.0%

稳定同位素技术在肉羊产地溯源中的应用

Application of Stable Isotope Technology in the Origin Traceability of Sheep

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