异三聚体G蛋白在UV-B诱导拟南芥气孔关闭中的作用

doi:10.3864/j.issn.0578-1752.2012.05.004

摘要/Abstract

摘要： 【目的】了解异三聚体G蛋白在紫外线B（UV-B）辐射诱导气孔关闭中的作用，为进一步阐明植物细胞转导UV-B辐射信号的机制提供依据。【方法】以拟南芥（Arabidopsis thaliana）野生型、G蛋白α亚基基因缺失突变体及其超表达株系和组成活化型株系为材料，并结合药理学试验，通过气孔开度分析确定G蛋白在0.5 W•m-2 UV-B辐射诱导拟南芥叶片气孔关闭中的作用。【结果】细菌毒素试验表明，G蛋白抑制剂百日咳毒素（PTX）能显著抑制UV-B诱导的气孔关闭，而其活化剂霍乱毒素（CTX）能模拟UV-B的作用诱导可见光下叶片气孔关闭。遗传学试验显示，UV-B不能诱导异三聚体G蛋白α亚基GPA1功能缺失突变体gpa1-1和gpa1-2的气孔关闭，却能诱导其超表达株系wGα和组成活化型株系cGα的气孔关闭，且cGα在可见光下气孔开度明显小于野生型，在UV-B辐射初期其气孔关闭速度明显快于野生型。【结论】异三聚体G蛋白α亚基参与了UV-B辐射诱导拟南芥叶片气孔关闭的信号转导过程。

关键词: 异三聚体G蛋白, 紫外线B辐射, 气孔运动, 拟南芥

Abstract: 【Objective】 The role of heterotrimeric G protein in UV-B-induced stomatal closure was analyzed to provide valuable evidence for further elucidating the mechanism of UV-B signaling transduction in plants.【Method】By using Arabidopsis (Arabidopsis thaliana) wild type, Gα-subunit-knockout mutants gpa1-1 and gpa1-2, wGα (a gpa1 overexpression line) and cGα (overexpression constitutively active form mutant of GPA1) combined with pharmacological analysis, the role of heterotrimeric G protein in 0.5 W•m-2 UV-B-induced stomatal closure was explored via stomatal bioassay.【Result】Pharmacological results showed that pertussis toxin (PTX), an inhibitor of G protein α subunit, inhibited UV-B-induced stomatal closure significantly. Meanwhile, cholera toxin (CTX), an activator of G protein α subunit, induced stomatal closure of leaves under light as well as UV-B radiation. Genetic data showed that UV-B-induced stomatal closure was completely impaired in the mutants gpa1-1 and gpa1-2, but not in the overexpression lines of wGα and cGα. In contrast, cGα showed not only smaller stomatal aperture than wild type under light, but also faster stomatal closure induced by UV-B than they did in the wild type.【Conclusion】 Results of this study strongly suggest the involvement of heterotrimeric G protein α subunit in the signaling transduction pathway of UV-B-induced stomatal closure in Arabidopsis.

Key words: heterotrimeric G protein, UV-B radiation, stomatal movement, Arabidopsis

马鲜歌, 贺军民. 异三聚体G蛋白在UV-B诱导拟南芥气孔关闭中的作用[J]. 中国农业科学, 2012, 45(5): 848-853.

MA Xian-Ge, HE Jun-Min. Role of Heterotrimeric G Protein in UV-B-Induced Arabidopsis Stomatal Closure[J]. Scientia Agricultura Sinica, 2012, 45(5): 848-853.

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参考文献

[1]Rozema J, Boelen P, Blokker P. Depletion of stratospheric ozone over the Antarctic and Arctic: responses of plants of polar terrestrial ecosystems to enhanced UV-B, an overview. Environmental Pollution, 2005, 137: 428-442.

[2]Jenkins G I. Signal transduction in responses to UV-B radiation. Annual Review of Plant Biology, 2009, 60: 407-431.

[3]Brown B A, Jenkins G I. UV-B Signaling pathways with different fluence-rate response profiles are distinguished in mature arabidopsis leaf tissue by requirement for UVR8, HY5, and HYH. Plant Physiology, 2008: 146: 576-588.

[4]Jones A M, Assmann S M. Plants: the latest model system for G-protein research. EMBO Reports, 2004, 5: 572-578.

[5]Milligan G, Kostenis E. Heterotrimeric G-proteins: a short history. British Journal of Pharmacology, 2006, 147: S46-S55.

[6]Okamoto H, Matsui M, Deng X W. Overexpression of the heterotrimeric G-protein α-subunit enhances phytochrome-mediated inhibition of hypocotyl elongation in Arabidopsis. The Plant Cell, 2001, 13: 1639-1651.

[7]Jones A M, Ecker J R, Chen J G. A reevaluation of the role of the heterotrimeric G protein in coupling light responses in Arabidopsis. Plant Physiology, 2003, 131: 1623-1627.

[8]Wang X Q, Ullah H, Jones A M, Assmann S M. G protein regulation of ion channels and abscisic acid signaling in Arabidopsis guard cells. Science, 2001, 292: 2070-2072.

[9]Zhang W, Jeon B W, Assmann S M. Heterotrimeric G-protein regulation of ROS signalling and calcium currents in Arabidopsis guard cells. Journal of Experimental Botany, 2011,62(7): 2371-2379.

[10]Fan L M, Zhang W, Chen J G, Taylor J P, Jones A M, Assmann S M. Abscisic acid regulation of guard-cell K+ and anion channels in Gβ- and RGS-deficient Arabidopsis lines. Proceedings of the National Academy of Sciences, USA, 2008, 105: 8476-8481.

[11]Pandey S, Wang R S, Wilson L, Li S, Zhao Z, Gookin T E, Assmann S M, Albert R. Boolean modeling of transcriptome data reveals novel modes of heterotrimeric G-protein action. Molecular Systems Biology, 2010, 6: 372.

[12]Fujisawa Y, Kato T, Ohki S, Ishikawa A, Kitano H, Sasaki T, Asahi T, Iwasaki Y. Suppression of the heterotrimeric G protein causes abnormal morphology, including dwarfism, in rice. Proceedings of the National Academy of Sciences, USA, 1999, 96: 7575-7580.

[13]Ullah H, Chen J G, Wang S, Jones A M. Role of a heterotrimeric G protein in regulation of Arabidopsis seed germination. Plant Physiology, 2002, 129: 897-907.

[14]Ullah H, Chen J G, Youg J C, Im K H, Sussman M R, Jones A M. Modulation of cell proliferation by heterotrimeric G protein in Arabidopsis. Science, 2001, 292: 2066-2069.

[15]Okamoto1 H, Göbel C, Capper R G, Saunders N, Feussner I, Knight M R. The α-subunit of the heterotrimeric G-protein affects jasmonate responses in Arabidopsis thaliana. Journal of Experimental Botany, 2009, 60(7): 1991-2003.

[16]Xing T, Higgins V J, Blumwald E. Identification of G proteins mediating fungal elicitor-induced dephosphorylation of host plasma membrane H+-ATPase. Journal of Experimental Botany, 1997, 48: 229-237.

[17]Rajasekhar V K, Lamb C, Dixon R A. Early events in the signal pathway for the oxidative burst in soybean cells exposed to avirulent Pseudomonas syringae pv glycinea. Plant Physiology, 1999, 120: 1137-1146.

[18]张占全, 宋水山, 张英春, 杨文香, 刘大群. 异三聚体G蛋白参与小麦抗叶锈病反应的研究. 中国农业科学, 2009, 42(1):117-123.

Zhang Z Q, Song S S, Zhang Y C, Yang W X, Liu D Q. Involvement of the heterotrimeric G protein in the defense responses of wheat to Puccinia triticina. Scientia Agricultura Sinica, 2009, 42(1):117-123. (in Chinese)

[19]Joo J H, Wang S, Chen J G, Jones A M, Fedoroff N V. Different signaling and cell death roles of heterotrimeric G protein α and β subunits in the Arabidopsis oxidative stress response to ozone. The Plant Cell, 2005, 17: 957-970.

[20]Chen Y L, Huang R, Xiao Y M, Lü P, Chen J, Wang X C. Extracellular calmodulin-induced stomatal closure is mediated by heterotrimeric G protein and H2O2. Plant Physiology, 2004, 136: 4096-4103.

[21]Li J H, Liu Y Q, Lü P, Lin H F, Bai Y, Wang X C, Chen Y L. A signaling pathway linking nitric oxide production to heterotrimeric G protein and hydrogen peroxide regulates extracellular calmodulin induction of stomatal closure in. Arabidopsis. Plant Physiology, 2009, 150: 114-124.

[22]Seo M, Cho C H, Lee Y I, Shin E Y, Park D, Bae C D, Lee J W, Lee E S, Juhnn Y S. Cdc42-dependent mediation of UV-induced p38 activation by G protein βγ subunits. The Journal of Biological Chemistry, 2004, 279(17): 17366-17375.

[23]Seo M, Lee M J, Heo J H, Lee Y I, Kim Y, Kim S Y, Lee E S, Juhnn Y S. G Protein βγ subunits augment UVB-induced apoptosis by stimulating the release of soluble heparin-binding epidermal growth factor from human keratinocytes. The Journal of Biological Chemistry, 2007, 282(34): 24720-24730.

[24]Seo M, Juhnn Y S. Gq protein mediates UVB-induced cyclooxygenase-2 expression by stimulating HB-EGF secretion from HaCaT human keratinocytes. Biochemical and Biophysical Research Communication, 2010, 393(2): 190-195.

[25]Coursol S, Fan L M, Le Stunff H, Spiegel S, Gilroy S, Assmann S M. Sphingolipid signalling in Arabidopsis guard cells involves heterotrimeric G proteins. Nature, 2003, 423: 651-654.

[26]Liu X, Yue Y, Li B, Nie Y, Li W, Wu W H, Ma L. A G protein-coupled receptor is a plasma membrane receptor for the plant hormone abscisic acid. Science, 2007, 315: 1712-1716.

[27]Dai Q J, Peng S B, Chavez A Q, Vergara B S. Effects of UVB radiation on stomatal density and opening in rice (Oryza sativa L.). Annals of Botany, 1995, 76: 65-70.

[28]Nogués S, Allen D J, Morison J I L, Baker N R. Characterization of stomatal closure caused by ultraviolet-B radiation. Plant Physiology, 1999, 121: 489-496.

[29]Jansen M A K, van den Noort R E. Ultraviolet-B radiation induces complex alterations in stomatal behaviour. Physiologia Plantarum, 2000, 110: 189-194.

[30]Kuryshev Y A, Naumov A P, Avdonin P V, Mozhayeva G N. Evidence for involvement of a GTP-binding protein in activation of Ca2+ influx by epidermal growth factor in A431 cells: effects of fluoride and bacterial toxins. Cellular Signalling, 1993, 5: 555-564.

[31]van Corven E J, Hordijk P L, Medema R H, Bos J L, Moolenaar W H. Pertussis toxin-sensitive activation of p21ras by G protein-coupled receptor agonists in fibroblasts. Proceedings of the National Academy of Sciences, USA, 1993, 90: 1257-1261.

[32]Pandey S, Assmann S M. The Arabidopsis putative G protein-coupled receptor GCR1 interacts with the G protein α subunit GPA1 and regulates abscisic acid signaling. The Plant Cell, 2004, 16: 1616-1632.

[33]He J M, Xu H, She X P, Song X G, Zhao W M. The role and the interrelationship of hydrogen peroxide and nitric oxide in the UV-B-induced stomatal closure in broad bean. Functional Plant Biology, 2005, 32: 237-247.

[34]He J M, Yue X Z, Wang R B, Zhang Y. Ethylene mediates UV-B-induced stomatal closure via peroxidase-dependent hydrogen peroxide synthesis in Vicia faba L. Journal of Experimental Botany, 2011, 62(8): 2657-2666.

[35]He J M, Zhang Z, Wang R B, Chen Y P. UV-B-induced stomatal closure occurs via ethylene-dependent NO generation in Vicia faba. Functional Plant Biology, 2011, 38(4): 293-302.