FLP/FRT位点特异性重组系统在高等真核生物中的研究进展

doi:10.3864/j.issn.0578-1752.2011.15.021

摘要/Abstract

摘要： 来源于酵母2 μm质粒的FLP/FRT位点特异性重组系统已经被广泛应用于拟南芥、水稻、小鼠、果蝇、线虫等高等真核模式生物，正逐渐成为转基因动植物研究领域进行基因操作的强有力工具。笔者综述了FLP/FRT系统的重组原理及其在高等真核生物中的应用，系统地概述了该系统在转基因植物、哺乳动物、昆虫以及其它相关高等真核模式生物中的研究现状，讨论了FLP/FRT系统在研究中存在的主要问题、应用前景和发展方向。

关键词: FLP/FRT系统, 位点特异性重组, 转基因, 高等真核生物

Abstract: FLP/FRT site-specific recombination system derived from 2 μm plasmid of yeast has been widely used in Arabidopsis thaliana, Oryza sativa Linnaeus, Mus musculus, Drosophila melanogaster, Caenorhabditis elegans and other higher eukaryotic organisms, and gradually become one of the powerful tools of genetic manipulation in transgenic animals and plants research areas. This review introduced the recombination principles of FLP/FRT system and its application in higher eukaryotes, and systematically summarized the main achievements of the system in transgenic plants, mammals, insects and other higher eukaryotic model organisms. In addition, the main problem, application prospect and developmental trend of the FLP/FRT system were discussed in this review.

Key words: FLP/FRT system, site-specific recombination, transgene, higher eukaryotes

赵爱春, 龙定沛, 谭兵, 许龙霞, 向仲怀. FLP/FRT位点特异性重组系统在高等真核生物中的研究进展[J]. 中国农业科学, 2011, 44(15): 3252-3263.

ZHAO Ai-Chun, LONG Ding-Pei, TAN Bing, XU Long-Xia, XIANG Zhong-Huai. Progress in Research of FLP/FRT Site-Specific Recombination System in Higher Eukaryotes[J]. Scientia Agricultura Sinica, 2011, 44(15): 3252-3263.

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