关键词: 香蕉, 脂氧合酶, 成熟, 跃变

Abstract:

【Objective】 The role of lipoxygenase (LOX) in banana fruit ripening was studied. 【Method】 Fruit ripened naturally and fruit treated with 1 000 μL?L-1 propylene for 10 h and 48 h after harvest were used as experimental materials. LOX activity and the expression patterns of MaLOX, MaACS, MaACO genes and ethylene production and its relation to fruit ripening were analyzed. 【Result】 The results showed that the peak of LOX activity and transcript accumulation firstly decreased, then increased, moreover, the increase in LOX activity and transcript accumulation appeared earlier than that of mRNA accumulations of MaACS and MaACO gene in the peel of propylene treated fruit at 48 h after harvest. Although propylene could induce the accumulations of MaACS and MaACO gene in the fruit of propylene treated fruit at 10h after harvest, but could not increase LOX activity and transcript accumulation, and thus could not accelerate banana fruit ripening.【Conclusion】These results suggest that LOX, ACS and ACO are likely to play a role in regulating the climacteric ethylene production and banana fruit ripening coordinately.

Key words: banana, lipoxygenase, ripening, climacteric