琅琊鸡及其配套系蛋壳质量、钙代谢生化指标和钙结合蛋白CaBP-D28k mRNA表达的比较

doi:10.3864/j.issn.0578-1752.2021.09.017

中国农业科学 ›› 2021, Vol. 54 ›› Issue (9): 2017-2026.doi: 10.3864/j.issn.0578-1752.2021.09.017

• 畜牧·兽医·资源昆虫 • 上一篇下一篇

琅琊鸡及其配套系蛋壳质量、钙代谢生化指标和钙结合蛋白CaBP-D28k mRNA表达的比较

张宁波¹(),韩照清¹,金太花¹,庄桂玉²,李炯奎³,郑全胜³,李永洙¹()

¹临沂大学农林科学学院,山东临沂 276005
²青岛西海岸新区农业农村局,山东青岛 266400
³山东琅琊鸡种业有限公司,山东临沂 276000

收稿日期:2020-04-13 接受日期:2020-09-03 出版日期:2021-05-01 发布日期:2021-05-10
通讯作者: 李永洙
作者简介:张宁波,E-mail：ningbo712@126.com。
基金资助:
山东省农业重大应用技术创新项目(20186243);琅琊鸡配套系构建与产业化推广项目(20171125)

Comparison Analysis on Eggshell Quality, Biochemical Index of Calcium Metabolism and Calcium Binding Protein CaBP-D28k mRNA Expression Between Langya Chicken and Its Synthetic Lines

ZHANG NingBo¹(),HAN ZhaoQing¹,JIN TaiHua¹,ZHUANG GuiYu²,LI JiongKui³,ZHENG QuanSheng³,LI YongZhu¹()

¹College of Agriculture and Forestry Science, Linyi University, Linyi 276005, Shandong
²Agricultural and Rural Bureau of Qingdao West Coast New District, Qingdao 266400, Shandong
³Shandong Langya Chicken Breed Industry Co., Ltd., Linyi 276000, Shandong

Received:2020-04-13 Accepted:2020-09-03 Online:2021-05-01 Published:2021-05-10
Contact: YongZhu LI

摘要/Abstract

摘要：

【目的】探讨影响琅琊鸡及其配套系蛋壳质量和钙代谢的差异因素,为琅琊鸡品种资源保护及开发提供理论依据。【方法】选取240日龄琅琊鸡及其浅麻羽色、深麻羽色配套系各180只,各品系随机分为6个重复,每个重复30只,在相同饲养条件下饲养至300日龄时各重复随机收集鸡蛋30枚,各重复随机选取6只,翅下静脉采集血样、屠宰后收集左腿胫骨及十二指肠、蛋壳腺、肾脏等组织样,用于检测蛋壳质量、钙、磷相关指标及钙结合蛋白CaBP-D28k mRNA的表达量。【结果】浅麻羽色配套系的蛋重显著低于深麻羽色配套系（P<0.05）,而蛋壳重低于其他品系（P>0.05）;另外,蛋壳厚度和蛋壳比率显著高于深麻羽色配套系（P<0.05）,蛋壳强度显著高于其他品系（P<0.05）;浅麻羽色配套系的蛋黄、蛋壳、胫骨中钙含量显著高于深麻羽色配套系（P<0.05）,蛋壳、胫骨中磷含量显著高于其他品系（P<0.05）;蛋壳中灰分含量浅麻羽色配套系最高,其次为琅琊鸡、深麻羽色配套系,而胫骨中灰分含量和胫骨重浅麻羽色配套系最高,其次为琅琊鸡、深麻羽色配套系,品系间均差异显著（P<0.05）;浅麻羽色配套系的体重、胫骨长度显著低于深麻羽色配套系（P<0.05）;浅麻羽色配套系的血钙、降钙素含量显著低于其他品系,而浅麻羽色配套系的血磷、碱性磷酸酶、甲状旁腺素含量显著高于其他品系,且品系间均差异显著（P<0.05）;浅麻羽色配套系的钙结合蛋白含量显著低于深麻羽色配套系（P<0.05）,而两个配套系的骨钙素显著高于琅琊鸡（P<0.05）;浅麻羽色配套系的十二指肠部位钙结合蛋白CaBP-D28k mRNA表达量显著高于其他品系（P<0.05）,而蛋壳腺部位表达量显著高于深麻羽色配套系（P<0.05）,肾脏部位钙结合蛋白表达量显著高于琅琊鸡（P<0.05）。【结论】在240—300日龄产蛋期,浅麻羽色配套系的血磷、碱性磷酸酶、甲状旁腺素、骨钙素等相关活性物质及十二指肠、肾脏、蛋壳腺部位钙结合蛋白CaBP-D28k mRNA表达水平高于其他品系,可能促进其小肠上段对钙磷的吸收和骨钙的释放、转运能力,影响蛋黄、蛋壳中矿物质的沉积和改善蛋壳、胫骨质量。

关键词: 琅琊鸡, 配套系, 蛋品质, 营养成分, 钙结合蛋白

Abstract:

【Objective】The aim of this research was to provide a theoretical basis for the conservation of Langya Chicken breed resources and the development of its synthetic lines based on the comparative study on eggshell quality and calcium metabolism of Langya Chicken and its two synthetic lines. 【Method】Each of one hundred and eighty 240-day-old birds of Langya Chicken and its two synthetic lines (Light linen and Dark linen synthetic lines), were randomly divided into 6 repeats, each with 30 replicates. When the chicken were raised to 300 days of age under the same breeding conditions, thirty eggs and six birds were randomly collected from each repeat, blood samples were taken from the veins under the wings, and tissue samples from the left leg tibia and duodenum, egg shell glands, and kidneys were collected after slaughter. Calcium- and phosphorus-related indicators and the expression levels of calcium binding protein CaBP-D28k mRNA were detected. 【Result】The results showed that the egg weight of the light linen synthetic line was significantly lower than that of the dark linen synthetic line (P<0.05), and the eggshell weight was lower than other lines (P>0.05). In addition, the eggshell thickness and eggshell ratio were significantly higher than those of the linen line (P<0.05), the strength of eggshell was significantly higher than that of other lines (P<0.05). The calcium content of egg yolk, eggshell, and tibia in light linen synthetic line was significantly higher than that in dark linen synthetic line (P<0.05). The content of phosphorus in the tibia was significantly higher than that of other lines (P<0.05), and the ash content in eggshells and tibia was the highest in the light linen synthetic line, followed by the Langya Chicken and the dark linen synthetic line (P<0.05). The body weight and tibia length of light linen synthetic line was significantly lower than those of dark linen synthetic line (P<0.05). The contents of plasma calcium and calcitonin in the light linen synthetic line were significantly lower than those in other lines, while the plasma phosphorus, alkaline phosphatase, and parathyroid hormone contents in the light linen synthetic line were significantly higher than those in other lines, and the differences between the lines were significant (P<0.05); the calcium binding protein content of the light linen synthetic line was significantly lower than that of the dark linen synthetic line (P<0.05). While the osteocalcin of the synthetic lines was significantly higher than that of the Langya Chicken (P<0.05). The expression level of calcium binding protein CaBP-D28k mRNA in duodenum of light linen synthetic line was significantly higher than that of other lines (P<0.05), and the expression level in eggshell gland was significantly higher than that of dark linen synthetic line (P<0.05). The expression level of calcium binding protein in kidney was significantly higher than that of Langya Chicken (P<0.05). 【Conclusion】The above results showed that the plasma phosphorus, alkaline phosphatase, parathyroid hormone, osteocalcin and other related active substances and calcium-binding protein CaBP-D28k mRNA expression level in duodenum, kidney, and shell glands of light linen synthetic lines was all higher than other lines during the 240-300 day-old laying period. which could promote the absorption of calcium and phosphorus in the upper small intestine and the release and transport of bone calcium, affect the deposition of minerals in egg yolk and eggshell, and improve the quality of eggshell and tibia.

Key words: Langya Chicken, synthetic line, egg quality, nutrient content, calcium binding protein

张宁波,韩照清,金太花,庄桂玉,李炯奎,郑全胜,李永洙. 琅琊鸡及其配套系蛋壳质量、钙代谢生化指标和钙结合蛋白CaBP-D28k mRNA表达的比较[J]. 中国农业科学, 2021, 54(9): 2017-2026.

ZHANG NingBo,HAN ZhaoQing,JIN TaiHua,ZHUANG GuiYu,LI JiongKui,ZHENG QuanSheng,LI YongZhu. Comparison Analysis on Eggshell Quality, Biochemical Index of Calcium Metabolism and Calcium Binding Protein CaBP-D28k mRNA Expression Between Langya Chicken and Its Synthetic Lines[J]. Scientia Agricultura Sinica, 2021, 54(9): 2017-2026.

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项目 Items	含量 Content (%)	营养水平 Nutrient levels	含量 Content
玉米 Corn	58.94	粗蛋白 CP	16.50
大豆粕Soybean meal	1.86	代谢能Me/(MJ·kg^-1) ²⁾	12.82
麸皮Rice bran	8.61	钙 Ca	3.90
玉米胚芽粕Corn germ meal	5.00	有效磷 P³⁾	0.44
干酒糟DDGS	10.00	盐NaCl	0.34
石粉 Limestone	5.07	赖氨酸 Lys	0.79
玉米蛋白粉Corn gluten meal	5.00	蛋氨酸 Met	0.39
磷酸氢钙 CaHPO₄	1.12	蛋+胱Met+Sys	0.66
DL-蛋氨酸 Met	0.03	苏氨酸Thr	0.59
盐(氯化钠) NaCl	0.32
70%赖氨酸Lys	0.50
70%氯化胆碱 Choline chloride	0.05
预混料 Premix¹⁾	3.50
总计 Total	100.00

基因 Gene	基因编号 GenBank ID	引物序列 Sequence of primers (sense/antisense) (5′-3′)	产物大小 Length (bp)
钙结合蛋白 CaBP-D28k	NM_205513	5′-TTAAATCTGCGTTGCTTCCATACA-3′ -3′	298
钙结合蛋白 CaBP-D28k	NM_205513	5′-GGCCCATCCTGCACTCCATAAC-3′ -3′	298
β肌动蛋白 β-actin	NM_205518	5′-CCCAAGGCCAACCGTGAGAAGAGT-3′ -3′	337
β肌动蛋白 β-actin	NM_205518	5′-CGAAGTCCAGGGCCACGTAGCAGAG-3′ -3′	337

项目 Items	琅琊鸡 Langya Chicken	浅麻羽色配套系 Light linen synthetic line	深麻羽色配套系 Dark linen synthetic line	P值 P value
产蛋率 Egg production (%)	68.23±5.35ab	73.45±6.32a	62.78±3.45b	0.027
蛋重 Egg weight (g)	49.58±4.25ab	46.09±2.55a	55.92±1.79b	0.035
蛋壳重Eggshell weight (g)	4.53±0.34	4.44±0.26	4.87±0.18	0.747
蛋壳强度 Eggshell strength (kg·cm^-2)	32.27±5.74a	39.65±8.34b	30.87±8.90a	0.041
蛋壳厚度Eggshell thickness (mm)	34.85±2.61ab	35.36±3.07a	32.26±2.32b	0.024
蛋黄比例Yolk ratio (%)	33.82±5.74	34.23±2.45	33.64±2.19	0.874
蛋壳比例 Eggshell ratio (%)	9.14±0.86ab	9.63±0.22a	8.71±0.43b	0.036

项目 Items		琅琊鸡 Langya Chicken	浅麻羽色配套系 Light linen synthetic line	深麻羽色配套系 Dark linen synthetic line	P值 P value
蛋黄 Yolk	钙Ca (%)	1.09±0.05ab	1.24±0.07a	1.06±0.02b	0.024
	磷 P (%)	2.40±0.05	2.28±0.04	2.47±0.07	0.821
	灰分Ash (%)	1.70±0.01	1.88±0.02	1.64±0.04	0.067
蛋壳 Eggshell	钙Ca (%)	32.15±2.14ab	33.31±2.62a	31.38±1.38b	0.042
	磷 P (%)	0.12±0.03a	0.14±0.02b	0.12±0.04a	0.045
	灰分Ash (%)	38.17±5.41a	40.38±6.24b	36.40±3.74c	0.037
胫骨 Tibia	钙Ca (%)	34.14±1.42a	38.45±4.11b	36.27±2.85c	0.044
	磷 P (%)	13.18±1.74a	15.78±2.14b	14.12±1.06c	0.032
	灰分Ash (%)	44.56±2.84a	48.26±6.42b	40.42±5.14c	0.067

项目 Items	琅琊鸡 Langya Chicken	浅麻羽色配套系 Light linen synthetic line	深麻羽色配套系 Dark linen synthetic line	P值 P value
开产体重 Birth weight (g)	1354.8±35.6a	1368.5±64.8a	1465.3±49.2b	0.037
300日龄体重 Weight at 300 days (g)	1625.71±24.45ab	1551.57±62.41a	1727.14±17.80b	0.011
胫骨重Tibial weight (g)	57.55±2.85a	59.15±3.74b	54.23±5.27c	0.046
胫骨长度Length (cm)	80.17±6.42ab	79.29±3.47a	83.00±2.46b	0.042
胫骨指数Tibial index (%)	3.54±0.14	3.81±0.31	3.14±0.10	0.072
胫骨密度TMD (kg·cm^-2)	14.22±2.41a	16.47±3.85b	14.87±2.67a	0.067

琅琊鸡及其配套系蛋壳质量、钙代谢生化指标和钙结合蛋白CaBP-D28k mRNA表达的比较

Comparison Analysis on Eggshell Quality, Biochemical Index of Calcium Metabolism and Calcium Binding Protein CaBP-D28k mRNA Expression Between Langya Chicken and Its Synthetic Lines

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项目 Items	琅琊鸡 Langya Chicken	浅麻羽色配套系 Light linen synthetic line	深麻羽色配套系 Dark linen synthetic line	P值 P value
血浆钙Plasma Ca (mmol·L^-1)	1.46±0.24a	1.32±0.31b	1.58±0.18c	0.028
血浆磷Plasma P (mmol·L^-1)	0.78±0.27a	0.72±0.56b	0.86±0.34c	0.043
碱性磷酸酶ALP (U·L^-1)	24.62±2.52a	29.50±2.74b	19.77±5.47c	0.024
甲状旁腺素PTH (pg·mL^-1)	75.68±14.12a	96.74±10.27b	87.26±9.51c	0.011
钙结合蛋白CaBP-D28k (ng·mL^-1)	17.14±1.21a	19.35±2.17b	16.84±1.72a	0.015
骨钙素BGP (ng·mL^-1)	2.98±0.52a	3.75±0.27b	3.64±0.85b	0.041
降钙素CT (pg·mL^-1)	16.24±1.40a	14.76±1.85b	17.68±2.40c	0.017

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