中国农业科学 ›› 2013, Vol. 46 ›› Issue (9): 1756-1761.doi: 10.3864/j.issn.0578-1752.2013.09.002

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

拟南芥AtBT4对灰葡萄孢的抗性机制分析

 郝丛丛, 杨萍, 陈展, 贾娇, 赵斌, 司贺龙, 韩建民, 邢继红, 董金皋   

  1. 1.河北农业大学生命科学学院/真菌毒素与植物分子病理学实验室,河北保定 071001
    2.河北省农林科学院果树研究所,石家庄 050061
    3.吉林省农林科学院植物保护研究所,吉林公主岭 050061
  • 收稿日期:2012-12-30 出版日期:2013-05-01 发布日期:2013-03-18
  • 通讯作者: 通信作者董金皋,Tel:0312-7528266;E-mail:dongjingao@126.com;通信作者邢继红,E-mail:xingjihong2000@126.com
  • 作者简介:郝丛丛,E-mail:hcc_622@163.com。杨萍,E-mail:10834855@qq.com。郝丛丛和杨萍为同等贡献作者
  • 基金资助:

    国家自然科学基金(31200203)、河北省自然科学基金(C2012204032)、高等学校博士学科点专项科研基金(20121302120007)

Mechanism Analysis of AtBT4 from Arabidopsis thaliana Against Botrytis cinerea

 HAO  Cong-Cong, YANG  Ping, CHEN  Zhan, JIA  Jiao, ZHAO  Bin, SI  He-Long, HAN  Jian-Min, XING  Ji-Hong, DONG  Jin-Gao   

  1. 1.College of Life Science, Agricultural University of Hebei/Mycotoxin and Molecular Plant Pathology Laboratory, Baoding 071001, Hebei
    2.Institute of Fruit Tree, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050061
    3.Institute of Plant Protection, Jilin Academy of Agricultural Sciences, Gongzhuling 050061, Jilin
  • Received:2012-12-30 Online:2013-05-01 Published:2013-03-18

摘要: 【目的】揭示AtBT4在拟南芥抗灰葡萄孢中与SA、JA信号途经的相互关系。【方法】利用RT-PCR技术,检测用SA、SA类似物BTH、JA、ACC及灰葡萄孢处理拟南芥野生型Col-0后其AtBT4的表达情况;检测经SA和JA处理后拟南芥SA、JA途径相关突变体AtBT4的表达情况;并检测接种灰葡萄孢后拟南芥野生型Col-0、bt4突变体和回复突变体抗病相关基因的表达情况。【结果】JA处理和接种灰葡萄孢后,拟南芥野生型Col-0中AtBT4的表达明显增强。但经JA处理后,JA不敏感突变体jar1的AtBT4表达变化趋势与野生型明显不同,AtBT4的表达水平不受JA诱导。SA信号途径相关突变体eds5、sid2和npr1中AtBT4的表达明显低于野生型,但变化趋势与野生型基本一致。bt4突变体中抗病相关基因PR1、PR4、PDF1.2和BIK1的表达明显低于野生型和回复突变体。【结论】AtBT4的表达受JA和灰葡萄孢的诱导,AtBT4突变影响抗病相关基因PR1、PR4、PDF1.2和BIK1的表达,AtBT4可能通过SA、JA信号途径影响拟南芥对灰葡萄孢的抗性。

关键词: AtBT4 , 拟南芥 , 灰葡萄孢 , 抗病信号途径

Abstract: 【Objective】The objective of this study is to reveal the interactions between AtBT4 gene and SA, JA signal pathways in Arabidopsis thaliana resistance to Botrytis cinerea.【Method】RT-PCR technology was used to analyze the expression of AtBT4 in Col-0 treated with SA, its analogues BTH, JA, ACC and B. cinerea, the expression of AtBT4 in various signals pathway mutants following SA or JA treatment and the expression of resistance-related genes in Col-0, bt4 and AtBT4 complemented plants. 【Result】The expression of AtBT4 in Col-0 treated with JA and B. cinerea was significantly enhanced. But no significant difference was observed in AtBT4 expression between the JA-insensitive jar1 mutants treated for 0 and 12 h with JA. The AtBT4 expression was significantly down-regulated in the eds5, sid2, and npr1 mutants compared with the Col-0 plants. Compared with the Col-0 and AtBT4 complemented plants, the expression of PR1, PR4, PDF1.2 and BIK1 genes was significantly down-regulated in the bt4 mutants. 【Conclusion】 The AtBT4 gene expression is induced by JA and B. cinerea and the AtBT4 gene deficiency alters expression of resistance-related genes, PR1, PR4, PDF1.2 and BIK1, suggesting the AtBT4 gene may be involved in the regulation of SA and JA singnal pathway to impact the resistance to B. cinerea in A. thaliana.

Key words: AtBT4 , Arabidopsis thaliana , Botrytis cinerea , disease-resistant pathway