关键词: 马铃薯, 晚疫病, StPK1启动子, TAIL-PCR

Abstract:

【Objective】 The objective of the experiment is to study the function of potato (Solanum tuberosum L.) protein kinase gene StPK1. 【Method】 The promoters of StPK1 were cloned by TAIL-PCR from leaves of three potato cultivars with distinct resistance types to late blight: quantitative, qualitative and susceptible. The promoter cloned from the qualitative resistance potato was fused to the GUS to construct the plant expression vector, and then the chimeric gene was transferred into Nicotiana benthamiana by Agrobacterium tumefaciens-mediated method. The transformed plants were treated with water, Phytophthora infestans and salicylic acid (SA), and the expression activity of the promoter was analyzed as well. 【Result】 The length of the promoters obtained are 922 bp, 929 bp, and 922 bp, respectively. The structure comparison indicated that all the promoters possess TATA-box, CAAT-box and the elements responsive to plant disease infection as well as those related to temporal and spatial expression. Their main differences were only observed in a little change of bases. And the results demonstrated that GUS was expressed in the leaves of transgenic tobacco treated with P. infestans and SA. 【Conclusion】 It showed that the promoter is active and pathogen- and SA-inducible.

Key words: potato, late blight, StPK1 promoter, TAIL-PCR