关键词: 猪, 资源家系, 群体结构, QTL定位, 模拟

Abstract:

【Objective】To optimize the QTL mapping design in pigs, the effects of different F2 schemes on QTL mapping were investigated using a simulation method. 【Method】 Using a simulation method, 18 population structures were simulated to evaluate the effects of the numbers of individuals in F0, F1 and F2 generations, the ratios of males to females in F1 and F2 generations and the QTL frequencies in Fo generation on QTL mapping. 【Result】With the increasing number of F2 individuals, the power of QTL detection increased, and the 95% confidence interval narrowed, while the estimate of the addictive effect of the QTL was downward. The reason for the downward biased estimator of addictive effect was that the QTL alleles were not alternatively fixed in the two founder breeds. As the proportion of F0 females increasing, the 95% confidence interval reduced. The power and the estimates were improved with the difference of QTL frequencies increasing in the founder populations. 【Conclusion】The number of F2 animals, the proportion of F0 females and the QTL allele frequencies in Fo generation had significant effects on QTL detection, but the numbers of F1 and F0 individuals and the proportion of F1 females had no significant effect on the QTL mapping.

Key words: pig, resource family, population structure, QTL mapping, simulation