关键词: 吸附剂, 黄曲霉毒素B1, 吸附能力

Abstract:

【Objective】 In this study, the in vitro sorption of aflatoxin B1(AFB1) onto different absorbents was characterized, and the in vitro assay result was verified by comparing growth performance and serum protein levels of broilers exposed to aflatoxin-contamination feed. Three absorbents were product A (the main component is yeast cell extracts), product B (the main component is HSCAS) and product C (the compound of yeast product and HSCAS). 【Method】 AFB1 binding capacity by three adsorbents at pH 2.0, 6.0, 8.0, simulated gastric fluid and simulated intestinal fluid condition, were compared. AFB1 binding speed by three absorbents in vitro conditions were compared. Stability of absorbent-AFB1 complexes in vitro conditions were studied. A total of 240 broilers were assigned to 8 treatments, and the effects of three adsorbents on growth performance and serum protein levels were compared. 【Result】 Product B showed the highest in vitro affinity for AFB1, followed by product C and product A. Product B bounded 97.69% of the AFB1 in solution in 10 min, and it remained over 96.03% in 60 min at pH 8.0. Product A and product C did not show the same efficacy as product B. The product B-AFB1 complex was much stronger than the other two kinds of complex in vitro condition. Feed intake and weight gain decreased (P＜0.05) and feed gain ratio increased (P＜0.05) in the treatment fed aflatoxin-contaminated feed as compared with the control. Serum total protein (TP), albumin (ALB), globulin (GLOB) levels were significantly decreased (P＜0.05). Product B (0.15%) increased growth performance and improved serum protein levels, product A and product C did not show so effectively as product B. 【Conclusion】 Three absorbents all showed binding capacity for AFB1 in some extent and product B could bind AFB1 more effectively than products A and C. These results indicated that product B could alleviate some of the toxic effect in broilers. It might prove to be beneficial in the management of aflatoxin-contaminated feedstuffs for poultry.

Key words: absorbent, AFB1, absorption efficacy