关键词: 噬菌体抗体库, 单链抗体, 羊

Abstract:

【Objective】 The purpose of this study is to construct the naive murine phage antibody library and acquire monoclonal antibodies against sheep inhibin by screening. 【Method】 The naive murine phage antibody library was constructed using phage display technology. The library was selected by using sheep inhibin as panning antigen by 3 rounds of binding-elution-enrichment procedure. The binding ability of expressed ScFv was detected by ELISA. 【Result】 The naive murine phage antibody library was established. Monoclonal antibodies against sheep inhibin were acquired by screening. Fifty-five out of 96 randomized clones were demonstrated to have binding capacities to sheep inhibin, with the positive ratio as much as 57%.【Conclusion】 The expressed ScFv had the ability to bind sheep inhibin. The production of genetic engineering antibody against sheep inhibin has provided a basis for further study. This strategy may provide an alternative newer approach to antibodies.

Key words: phage antibody library, ScFv, sheep