中国农业科学 ›› 2007, Vol. 40 ›› Issue (5): 1024-1032 .

• 畜牧·兽医·资源昆虫 • 上一篇    下一篇

苯并咪唑氨基甲酸酯类药物抗猪囊尾蚴的作用靶点

李庆章,郝艳红,高学军,刘永杰,高文学,赵 冰   

  1. 东北农业大学生命科学学院
  • 收稿日期:2006-03-23 修回日期:1900-01-01 出版日期:2007-05-10 发布日期:2007-05-10

Target Position and Effective Mechanism of benzimidazole carbamate against Cysticerci cellulosae

  

  1. 东北农业大学生命科学学院
  • Received:2006-03-23 Revised:1900-01-01 Online:2007-05-10 Published:2007-05-10

摘要: 【目的】研究苯并咪唑氨基甲酸酯类抗囊药物对猪带绦虫囊尾蚴的作用靶点,为药物的类型衍化和新药开发提供理论依据。【方法】测定体外培养的和猪体内的囊尾蚴于阿苯哒唑和奥芬哒唑分别作用后能量代谢途径物质含量及其关键酶活性变化。【结果】阿苯哒唑和奥芬哒唑对体外培养猪六钩蚴、未成熟期和成熟期猪囊尾蚴,以及体内发育未成熟期和成熟期猪囊尾蚴的糖无氧分解途径、丙酮酸羧化支路与逆向三羧酸循环途径均有一定程度抑制作用,代偿性促进糖异生及蛋白质、脂类和核酸分解途径,葡萄糖吸收受阻。阿苯哒唑和奥芬哒唑体外非竞争性抑制延胡索酸还原酶复合体活性。【结论】并咪唑氨基甲酸酯类抗囊药物非竞争性抑制猪囊尾蚴FR复合体活性,抑制葡萄糖的摄取。

关键词: 阿苯哒唑, 奥芬哒唑, 猪囊尾蚴, 作用靶点, 能量代谢

Abstract: Using the technique to detect enzyme activity and metabolite content, the activity of key enzyme in pathway of energy metabolism was studied after drugs were applied. The target position of drug benzimidazole carbamate against Cysticerci cellulosae is fumaric reductase(FR)complex. The effective mechanism is the inhibition of uptaking glucose, noncompetitive inhibition of activity of fumaric reductase(FR)complex, at last disorder of energy metabolism, burnout of energy, and lead to death of Cysticerci cellulosae.

Key words: Cysticerci cellulosae, target position, effective mechanism, energy metabolism