关键词: 昆虫, 抗真菌肽, 毕赤酵母, 分泌型表达, 抗真菌活性鉴定

Abstract: The antifungal peptides drosomycin (Drs) and its isoform drosomycin-like C (Drs-lC) from Drosophila melanogaster and thanatin from Podisus maculiventris have broad and potent activity to filamentous fungi. The secretive expression of these antifungal peptide genes in yeast will provide the possibility to use their expressive products as protective regents on fruit, vegetable, food and agricultural products directly. The three genes of drs, Drs-lC and thanatin were cloned into pPICZα, a secretive vector of yeast. The recombinant vectors which were named pPICZαA-drs、pPICZαA-Drs-lC and pPICZα-Thanatin were integrated into the genome of Pichia pastoris by electric transformation method. The recombinant Pichia pastoris was induced to express antifungal peptide by methanol. The expressive products of the three recombinant showed antifungal activity to 5 of 6 tested fungi species and the expressive product of thanatin also had strong activity to the tested bacteria.

Key words: secretive expression, activity detection, insect, antimicrobial peptides, Pichia pastoris