关键词: 猪, SLA-DQA, 第四外显, SNP, PCR-RFLP, 关联分析

Abstract: A novel SNP at the nucleotide 722 of coding sequence, which caused shift mutation, were detected using PCR-RFLP. This study was designed to investigate the effect of DQA on pig economic traits. The results of genotypes in six Chinese indigenous breeds and Large White showed that there exist polymorphism excepting Xiaomeishan Pigs and Large White. The differences in genotype frequency were scored using the χ2 value among the 6 indigenous breeds. The genotype distributions were also analyzed in Large White, Landrace, Tongcheng, Landrace (Large White Tongcheng) and Large White (Landrace Tongcheng), and the association analysis between genotype and traits was performed by GLM. The results showed that the genotypes have significant correlations with average backfat thickness, backfat thickness between 6th and 7th ribs, percentage of leaf and caul and eye muscle width (P<0.05), and nearly significant correlation levels with backfat at the loin (P=0.06). However, there were no significant associations between DQA genotype and growth traits.

Key words: Pig, SLA-DQA, Exon 4, SNP, PCR-RFLP, Association analysis