中国农业科学

• • 上一篇    

绵羊ADIPOQ多态性与生长性状的关联分析

梁鹏,张天闻,孟科,邵顺成,邹诗凡,荣轩,强浩,冯登侦   

  1. 宁夏大学农学院,银川 750021
  • 发布日期:2021-09-13

Association Analysis of the ADIPOQ Variation with Sheep Growth Traits

LIANG Peng, ZHANG TianWen, MENG Ke, SHAO ShunCheng, ZOU ShiFan, RONG Xuan, QIANG Hao, FENG DengZhen   

  1. College of Agricultural, Ningxia University Yinchuan 750021
  • Online:2021-09-13

摘要: 【目的】探讨ADIPOQ遗传变异对绵羊生长性状的影响,找到与宁夏优质肉羊培育中生长性状相关的分子遗传标记,以达到分子辅助育种的目的。【方法】首先利用液相捕获测序技术获得ADIPOQ在杜泊羊、滩羊和小尾寒羊中的变异位点,同时采集3833个品种的不同杂交后代群体耳组织,采用飞行质谱技术对确定的SNPs位点进行基因分型检测,并使用Haploview软件对多态性位点进行连锁不平衡分析和构建单倍型,与绵羊初生和3月龄的生长性状进行关联分析。【结果】共筛选到7SNPs位点,且7个位点在杂交后代群体中均表现出多态性,SNP1—SNP7位点的优势基因型分别为CCGGGGCTAGGGAA,优势等位基因分别为CGGCGGAX2检验发现,所有位点均处于Hardy-Weinberg平衡状态(除SNP7位点在所有个体中偏离了平衡)。SNP1F2代除外)、SNP4SNP5SNP6位点在各杂交后代及所有个体中均处于中度多态(0.25≤PIC<0.50),SNP2和SNP3F2代及SNP7H1代群体中均处于中度多态(0.25≤PIC<0.50),而在其它群体中处于低度多态(PIC<0.25)。连锁不平衡分析发现,SNP2-SNP3SNP5-SNP6形成了两处强连锁,均构建出3种单倍型,组合后分别形成46种基因型,其中优势基因型分别为H1H1H4H6。将SNP2-SNP3SNP5-SNP6形成的单倍型再组合后产生13种基因型,其优势基因型为H1H1H4H6。单个位点关联分析表明:SNP1位点在F1代群体中,GG基因型的初生胸围显著高于CG基因型(P<0.05),在H2代群体中,CC基因型的初生体斜长显著高于CG基因型(P<0.05)。SNP2位点在H1代群体中,CC基因型的初生体高显著低于CGGG基因型(P<0.05)。SNP3位点在F1代群体中,AG基因型的3月龄体重显著高于GG基因型(P<0.05),在H1代群体中,AA基因型的初生体高显著低于AGGG基因型(P<0.05)。SNP4位点在F1代群体中,CC基因型的3月龄体重显著高于CTTT基因型(P<0.05),在F2代群体中,CT基因型的初生体重和初生胸围显著高于TT基因型(P<0.05),在H1代群体中,TT基因型的初生体重显著低于CCCT基因型(P<0.05),在H2代群体中,TT基因型的初生体高和初生体斜长显著高于CT基因型(P<0.05)。SNP5位点在F2代群体中,AA基因型的初生体高和初生体斜长显著高于GG基因型(P<0.05)。SNP6位点的不同基因型在F2代、H1代和H2代的初生体重、体高、体斜长、3月龄体高胸围上均有不同程度的显著差异(P<0.05)。SNP7位点在H2代群体中,AA基因型的初生体斜长显著高于GA基因型(P<0.05)。联合所有群体进行分析时发现,SNP2位点CG基因型的初生体重和体斜长显著高于GG基因型(P<0.05),SNP4位点TT基因型的初生体重显著低于CCCT基因型(P<0.05),SNP6位点AA基因型的初生体重显著高于GGGA基因型(P<0.05)、初生胸围显著高于GG基因型(P<0.05),其它5个位点的基因型生长性状均无显著差异(P>0.05)。单倍型组合关联分析发现:H2H3基因型的初生体重和初生体斜长显著高于其它基因型(P<0.05);而SNP5-SNP6单倍型组合中,H5H5基因型的初生体重显著高于H5H6H6H6P<0.05)、初生体高显著高于H5H6P<0.05初生胸围显著高于H6H6P<0.05),H4H4基因型的3月龄体重显著高于H5H5基因型(P<0.05)、3月龄体高和体斜长显著高于H4H6基因型(P<0.05)、3月龄胸围显著高于H4H5H5H6H5H5基因型(P<0.05)。SNP2-SNP3SNP5-SNP6形成的单倍型再组合后,H2H3H4H4基因型的初生体重、体高、体斜长和胸围最高,与其它基因型有不同程度的差异,H1H1H4H63月龄体斜长显著低于H1H2H4H4H2H2H4H4基因型(P<0.05)。【结论】ADIPOQ的遗传变异对绵羊的生长性状具有不同程度的影响,本研究的7SNPs位点可以作为宁夏优质肉羊选育中生长性状的潜在分子标记。


关键词: 绵羊, ADIPOQ, 多态性, 生长性状, 单倍型

Abstract: ObjectiveThe aim of the study was to explore the effects of genetic variation of ADIPOQ on growth traits of sheep, and to find the molecular genetic markers related to growth traits in Ningxia high-quality mutton sheep breeding, so as to achieve the purpose of molecular assisted breeding. MethodFirstly, the mutation sites of ADIPOQ in Dupo sheep, Tan sheep and small-tailed Han sheep were obtained by Allegro Targeted Genotyping. At the same time, ear tissues of 383 different hybrid progenies of three breeds were collected. The SNPs were genotyped by Sequenom Mass ARRAY?SNP. Haploview was used to analysis linkage disequilibrium and construct haplotypes of the polymorphic loci. Association analysis between the SNPs in ADIPOQ with growth traits of newborn and 3-month-old sheep.ResultA total of 7 SNPs were screened, and 7 SNPs showed polymorphism in the hybrid population. The dominant genotypes of SNP1-SNP7 were CC, GG, GG, CT, AG, GG and AA, and the dominant alleles were C, G, G, C, G, G and A. X2 test showed that all loci were in Hardy Weinberg equilibrium (except SNP7 site deviated from the equilibrium in all individuals). SNP1 (except F2), SNP4, SNP5 and SNP6 were moderately polymorphic (0.25 ≤ PIC < 0.50), SNP2 and SNP3 were moderately polymorphic (0.25 ≤ PIC < 0.50) in F2 and SNP7 were moderately polymorphic (0.25 ≤PIC < 0.50) in H1, and were low polymorphic (PIC < 0.25) in other populations. The results of linkage imbalance analysis showed that SNP2-SNP3 and SNP5-SNP6 formed two strong linkage, each of which constructed three haplotypes, and formed 4 and 6 genotypes respectively after combination, among which the dominant genotype was H1H1 and H4H6. 13 genotypes were produced after the haplotypes formed by SNP2-SNP3 and SNP5-SNP6 were recombined, and the dominant genotype was H1H1H4H6. Single SNP correlation analysis revealed that: SNP1 site in F1 population, the primary chest of GG genotype was significantly higher than that of CG genotype(P<0.05), and in H2 population, the primary body length of CC genotype was significantly higher than that of GG genotype(P<0.05). SNP2 site in H1 population, the primary body height of CC genotype was significantly lower than that of CG and GG genotypes (P < 0.05). SNP3 site in F1 population, the three months body weight of AG genotype was significantly higher than that of GG genotype, and in H1 population, the primary body height of AA genotype was significantly lower than AG and GG genotypes (P<0.05). SNP4 site in F1 population, the three months weight of CC genotype was significantly higher than that of CT and TT genotypes(P<0.05), in F2 population, the primary weight and primary chest of CT genotype were significantly higher than that of TT genotype(P<0.05). and that of TT genotype was significantly lower than that of CC and CT genotype(P<0.05) in H1 population, and in H2 population, the primary body height and primary body length of TT genotype were significantly higher than that of CT genotype(P<0.05). SNP5 site in F2 population, AA genotype had significantly higher primary body height and primary body length than GG genotype(P<0.05). There were significant differences in primary weight, body height, body length, three months body height and chest among different genotypes of SNP6(P<0.05). SNP7 site in H2 population, AA genotype had significantly higher primary body length than GA genotype(P<0.05).When combined with all groups, we found that , the primary weight and body length of CG genotype at SNP2 were significantly higher than GG genotype(P<0.05), the primary weight of TT genotype at SNP4 was significantly lower than CC and CT genotypes(P<0.05), the primary weight of AA genotype at SNP6 was significantly higher than GG and GA genotype(P<0.05), and the primary chest was significantly higher than GG genotypes(P<0.05). There was no significant difference in growth traits among the other five genotypes(P>0.05). The results of haplotype association analysis showed that the primary weight and primary body length of H2H3 genotype were significantly higher than those of other genotypes(P<0.05), while the primary weight of H5H5 genotype was significantly higher than that of H5H6 and H6H6(P<0.05), the primary body height was significantly higher than that of H5H6(P<0.05), the primary chest was significantly higher than that of H6H6(P<0.05), and the three months weight of H4H4 genotype was significantly higher than that of H5H5 genotype(P<0.05), the body weight and body length at three months were significantly higher than those of H4H6 genotype(P<0.05), and the chest of three months was significantly higher than that of H4H5,H5H6 and H5H5 genotype(P<0.05). After SNP2-SNP3 and SNP5-SNP6 haplotypes were recombined, the primary weight, body height, body length and chest of H2H3H4H4 genotype were the highest, which were different from other genotypes. The body length of H1H2H4H4 and H2H2H4H4 genotypes(P<0.05).【Conclusion】The results showed that the different SNPs and combined genotypes in ADIPOQ had effect on sheep different growth traits, the seven SNPs in this study can be used as potential molecular markers for growth traits in Ningxia high quality mutton sheep breeding.


Key words: sheep, ADIPOQ, polymorphisms, growth traits, haplotype