菌核青霉HY5中3,5-二甲基苔色酸类混源萜的结构及其除草活性

doi:10.3864/j.issn.0578-1752.2025.22.009

中国农业科学 ›› 2025, Vol. 58 ›› Issue (22): 4673-4687.doi: 10.3864/j.issn.0578-1752.2025.22.009

菌核青霉HY5中3,5-二甲基苔色酸类混源萜的结构及其除草活性

蔡海林¹(), 黄馨瑢², 曾维爱¹, 彭治鑫², 赵阿娟¹, 薄纯斌¹, 向世鹏¹, 高鹏程³, 张成省², 赵栋霖²()

¹ 湖南省烟草公司长沙市公司，长沙 410011
² 中国农业科学院烟草研究所/国家农业环境微生物种质资源库（山东）/青岛市农业微生物种业技术创新中心，山东青岛 266101
³ 山东临沂烟草有限公司，山东临沂 276001

收稿日期:2025-08-12 接受日期:2025-09-27 出版日期:2025-11-16 发布日期:2025-11-21
通信作者:
赵栋霖，E-mail：zhaodonglin@caas.cn。
联系方式: 蔡海林，E-mail：hailincai@126.com。
基金资助:
国家自然科学基金(32470420); 中国烟草总公司重大科技项目(110202401015(LS-05)); 青岛市自然科学基金原创探索项目(23-2-1-183-zyyd-jch)

Structure and Herbicidal Activity of 3,5-Dimethylorsellinic Acid-Derived Meroterpenoids from Penicillium sclerotiorum HY5

CAI HaiLin¹(), HUANG XinRong², ZENG WeiAi¹, PENG ZhiXin², ZHAO AJuan¹, BO ChunBin¹, XIANG ShiPeng¹, GAO PengCheng³, ZHANG ChengSheng², ZHAO DongLin²()

¹ Changsha Tobacco Company of Hunan Province, Changsha 410011
² Institute of Tobacco Research, Chinese Academy of Agricultural Sciences/National Agricultural Environmental Microbial Resource Bank (Shandong)/Qingdao Agricultural Microbial Seed Industry Technology Innovation Center, Qingdao 266101, Shandong
³ Shandong Linyi Tobacco Co., Ltd., Linyi 276001, Shandong

Received:2025-08-12 Accepted:2025-09-27 Published:2025-11-16 Online:2025-11-21

摘要/Abstract

摘要：

【背景】3，5-二甲基苔色酸（3，5-dimethylorsellinic acid，DMOA）类混源萜化学结构多样，生物活性广泛，然而对其农用生物活性鲜有报道。化学除草剂易造成杂草抗性增强并引发环境问题，亟需研发新型生物除草剂。【目的】从菌核青霉（Penicillium sclerotiorum）HY5中挖掘DMOA类混源萜，评价其除草活性，初步阐明其作用机制，为开发新型天然产物除草剂提供先导分子。【方法】运用正/反相硅胶柱层析、薄层层析、Sephadex LH-20凝胶柱层析和半制备高效液相色谱（HPLC）等方法分离DMOA类混源萜，采用核磁（NMR）、质谱（MS）等方法对化合物进行结构鉴定；运用种子萌发抑制法测定化合物对牛筋草（Eleusine indica）、狗尾草（Setaria viridis）、反枝苋（Amaranthus retroflexus）和三叶草（Trifolium repens）的除草活性；采用非线性回归分析方法，计算活性化合物的EC₅₀；采用转录组-代谢组联用的方法初步分析活性化合物的除草作用机制。【结果】从HY5发酵提取物中获得了9个DMOA类混源萜，分别为berkeleydione（1）、preaustinoid A3（2）、miniolutelide B（3）、22-epoxyberkeleydione（4）、22-deoxyminiolutelide B（5）、miniolutelide C（6）、berkeleyacteal（7）、preaustinoid A2（8）和berkeleyacetal A（9）。化合物4对双子叶杂草三叶草表现出明显的促生效果，对胚根和胚芽生长的促进率分别达到30.5%和55.1%。化合物3、4、5和6对单子叶杂草狗尾草和牛筋草种子具有显著的萌发抑制作用，其中化合物6的除草活性最强，抑制牛筋草胚根和胚芽伸长的EC₅₀分别达到19.1和17.9 μg·mL^-1，强于对照药剂草甘膦（30.9和66.2 μg·mL^-1）。同时发现，化合物对胚根伸长的抑制效果优于对胚芽效果。进一步研究表明，化合物6通过双重调控机制抑制杂草种子萌发：一方面，降解茉莉酸信号通路中的JAZ蛋白，从而促进脱落酸合成；另一方面，干扰色氨酸代谢途径，导致血清素的异常积累，最终抑制杂草的生长。【结论】DMOA类混源萜结构新颖，对单子叶杂草具有明显的除草活性，有望开发成为新型天然产物除草剂。

关键词: 菌核青霉, DMOA类混源萜, 结构鉴定, 除草活性, 作用机制, 茉莉酸信号通路, 色氨酸代谢途径

Abstract:

【Background】3,5-Dimethylorsellinic acid (DMOA)-derived meroterpenoids exhibit diverse chemical structures and broad biological activities. However, their agricultural bioactivities have rarely been reported. Chemical herbicides often lead to increased weed resistance and environmental problem, highlighting the urgent need for the development of novel bioherbicides. 【Objective】The objective of this study is to explore DMOA-derived meroterpenoids from Penicillium sclerotiorum HY5, evaluate their herbicidal activity, and preliminarily elucidate their modes of action, thus to provide lead molecules for the development of novel natural-product herbicides. 【Method】DMOA-derived meroterpenoids were isolated using normal/ reversed-phase silica gel column chromatography (CC), thin-layer chromatography, Sephadex LH-20 gel CC, and semi- preparative high-performance liquid chromatography (HPLC). The structures of the compounds were identified via nuclear magnetic resonance (NMR) and mass spectrometry (MS). The herbicidal activity of the compounds was assessed using a seed germination inhibition assay against Eleusine indica, Setaria viridis, Amaranthus retroflexus, and Trifolium repens. The EC₅₀ values for the bioactive compounds were determined by nonlinear regression analysis of dose-response curves. The herbicidal mechanism of the bioactive compounds was analyzed using a combined transcriptomic-metabolomic approach. 【Result】Nine DMOA-derived meroterpenoids were obtained from the HY5 fermentation extract, namely berkeleydione (1), preaustinoid A3 (2), miniolutelide B (3), 22-epoxyberkeleydione (4), 22-deoxyminiolutelide B (5), miniolutelide C (6), berkeleyacteal (7), preaustinoid A2 (8), and berkeleyacetal A (9). Compound 4 showed a significant promoting effect on the growth of the dicotyledonous weed T. repens, with the promotion rates of radicle and germ growth reaching 30.5% and 55.1%, respectively. Compounds 3, 4, 5, and 6 exhibited significant inhibitory effects on the germination of monocot weed seeds (S. viridis and E. indica). Notably, compound 6 showed the strongest herbicidal activity, with EC₅₀ values of 19.1 and 17.9 μg·mL^-1 for radicle and germ elongation of E. indica respectively, even stronger than the positive control glyphosate (30.9 and 66.2 μg·mL^-1). Additionally, the compounds demonstrated more potent herbicidal activity against radicle elongation compared to germ. Further studies revealed that compound 6 inhibits weed germination through dual regulatory mechanisms: (1) degrading JAZ proteins in the jasmonic acid signaling pathway, thereby promoting abscisic acid synthesis, and (2) disrupting the tryptophan metabolism pathway, leading to abnormal serotonin accumulation and ultimately suppressing weed growth. 【Conclusion】DMOA-derived meroterpenoids possess novel structures and exhibit significant inhibitory effects on monocot weeds, showing promise for development as new natural-product herbicides.

Key words: Penicillium sclerotiorum, DMOA-derived meroterpenoids, structural identification, herbicidal activity, modes of action, jasmonic acid signaling pathway, tryptophan metabolism pathway

蔡海林, 黄馨瑢, 曾维爱, 彭治鑫, 赵阿娟, 薄纯斌, 向世鹏, 高鹏程, 张成省, 赵栋霖. 菌核青霉HY5中3,5-二甲基苔色酸类混源萜的结构及其除草活性[J]. 中国农业科学, 2025, 58(22): 4673-4687.

CAI HaiLin, HUANG XinRong, ZENG WeiAi, PENG ZhiXin, ZHAO AJuan, BO ChunBin, XIANG ShiPeng, GAO PengCheng, ZHANG ChengSheng, ZHAO DongLin. Structure and Herbicidal Activity of 3,5-Dimethylorsellinic Acid-Derived Meroterpenoids from Penicillium sclerotiorum HY5[J]. Scientia Agricultura Sinica, 2025, 58(22): 4673-4687.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2025.22.009

https://www.chinaagrisci.com/CN/Y2025/V58/I22/4673

图/表 8

表1

图1

图2

图3

表2

表3

图4

图5

参考文献 39

[1]	李香菊. 近年我国农田杂草防控中的突出问题与治理对策. 植物保护, 2018, 44(5): 77-84.
	LI X J. Main problems and management strategies of weeds in agricultural fields in China in recent years. Plant Protection, 2018, 44(5): 77-84. (in Chinese)
[2]	STOKSTAD E. Jury verdicts cloud future of popular herbicide. Science, 2019, 364(6442): 717-718.
[3]	强胜. 中国杂草生物学研究的新进展. 杂草学报, 2018, 36(2): 1-9.
	QIANG S. New progresses on studies of weed biology in China. Journal of Weed Science, 2018, 36(2): 1-9. (in Chinese)
[4]	DUKE S O, DAYAN F E. The search for new herbicide mechanisms of action: Is there a ‘holy grail’? Pest Management Science, 2022, 78(4): 1303-1313.
[5]	邵旭升, 杜少卿, 李忠, 钱旭红. 中国绿色农药的研究和发展. 世界农药, 2020, 42(4): 16-24.
	SHAO X S, DU S Q, LI Z, QIAN X H. Research and development of green pesticides in China. World Pesticides, 2020, 42(4): 16-24. (in Chinese)
[6]	TAKANO H K, DAYAN F E. Glufosinate-ammonium: A review of the current state of knowledge. Pest Management Science, 2020, 76(12): 3911-3925.
[7]	刘璐, 朱哲远, 李颖曦, 王颉, 彭迪. 微生物除草剂的研究进展. 生物技术通报, 2024, 40(9): 161-171.
	LIU L, ZHU Z Y, LI Y X, WANG J, PENG D. Research progress in microbial herbicides. Biotechnology Bulletin, 2024, 40(9): 161-171. (in Chinese)
[8]	CIMMINO A, MASI M, EVIDENTE M, SUPERCHI S, EVIDENTE A. Fungal phytotoxins with potential herbicidal activity: Chemical and biological characterization. Natural Product Reports, 2015, 32(12): 1629-1653.
[9]	SPARKS T C, DUKE S O. Structure simplification of natural products as a lead generation approach in agrochemical discovery. Journal of Agricultural and Food Chemistry, 2021, 69(30): 8324-8346.
[10]	VURRO M, BOARI A, CASELLA F, ZONNO M C. Fungal phytotoxins in sustainable weed management. Current Medicinal Chemistry, 2018, 25(2): 268-286.
[11]	ZHAO M, TANG Y, XIE J, ZHAO Z, CUI H. Meroterpenoids produced by fungi: Occurrence, structural diversity, biological activities, and their molecular targets. European Journal of Medicinal Chemistry, 2021, 209: 112860.
[12]	BARRA L, ABE I. Chemistry of fungal meroterpenoid cyclases. Natural Product Reports, 2021, 38(3): 566-585.
[13]	QI B, LIU X, MO T, ZHU Z, LI J, WANG J, SHI X, ZENG K, WANG X, TU P, ABE I, SHI S. 3,5-Dimethylorsellinic acid derived meroterpenoids from Penicillium chrysogenum MT-12, an endophytic fungus isolated from Huperzia serrata. Journal of Natural Products, 2017, 80(10): 2699-2707.
[14]	VALIANTE V, MATTERN D J, SCHUFFLER A, HORN F, WALTHER G, SCHERLACH K, PETZKE L, DICKHAUT J, GUTHKE R, HERTWECK C, NETT M, THINES E, BRAKHAGE A A. Discovery of an extended austinoid biosynthetic pathway in Aspergillus calidoustus. ACS Chemical Biology, 2017, 12(5): 1227-1234.
[15]	WANG W, WANG M, WANG X B, LI Y Q, DING J L, LAN M X, GAO X, ZHAO D L, ZHANG C S, WU G X. Phytotoxic azaphilones from the mangrove-derived fungus Penicillium sclerotiorum HY5. Frontiers in Microbiology, 2022, 13: 880874.
[16]	HUANG X R, JIANG C N, WANG H S, GU G, WANG M, SUI X N, SI T, ZHANG Z F, ZHANG P, ZHAO D L. Herbicidal sorbicillinoid analogs cause lignin accumulation in Aspergillus aculeatus TE- 65L. Journal of Agricultural and Food Chemistry, 2024, 72(38): 21102-21111.
[17]	代义乐, 陈可可, 刘东丽, 蔡加岭, 吴越, 张辉, 张继. 链霉菌YJ-81中活性成分的分离、鉴定及除草活性. 农药学学报, 2023, 25(5): 1104-1112.
	DAI Y L, CHEN K K, LIU D L, CAI J L, WU Y, ZHANG H, ZHANG J. Isolation and characterization of a herbicidal component produced by Streptomyces sp. YJ-81. Chinese Journal of Pesticide Science, 2023, 25(5): 1104-1112. (in Chinese)
[18]	REN Y, YU G, SHI C, LIU L, GUO Q, HAN C, ZHANG D, ZHANG L, LIU B, GAO H, et al. Majorbio Cloud: A one-stop, comprehensive bioinformatic platform for multiomics analyses. iMeta, 2022, 1(2): e12.
[19]	CHEN J C, HUANG H J, WEI S H, HUANG Z F, WANG X, ZHANG C X. Investigating the mechanisms of glyphosate resistance in goosegrass (Eleusine indica (L.) Gaertn.) by RNA sequencing technology. The Plant Journal, 2017, 89(2): 407-415.
[20]	STIERLE D B, STIERLE A A, HOBBS J D, STOKKEN J, CLARDY J. Berkeleydione and berkeleytrione, new bioactive metabolites from an acid mine organism. Organic Letters, 2004, 6(6): 1049-1052.
[21]	LO H C, ENTWISTLE R, GUO C J, AHUJA M, SZEWCZYK E, HUNG J H, CHIANG Y M, OAKLEY B R, WANG C C C. Two separate gene clusters encode the biosynthetic pathway for the meroterpenoids austinol and dehydroaustinol in Aspergillus nidulans. Journal of the American Chemical Society, 2012, 134(10): 4709-4720.
[22]	IIDA M, OOI T, KITO K, YOSHIDA S, KANOH K, SHIZURI Y, KUSUMI T. Three new polyketide-terpenoid hybrids from Penicillium sp. Organic Letters, 2008, 10(5): 845-848.
[23]	HOANG T P T, ROULLIER C, BOUMARD M C, DU PONT T R, NAZIH H, GALLARD J F, POUCHUS Y F, BENIDDIR M A, GROVEL O. Metabolomics-driven discovery of meroterpenoids from a mussel-derived Penicillium ubiquetum. Journal of Natural Products, 2018, 81(11): 2501-2511.
[24]	LI J, YANG X, LIN Y Y, YUAN J, LU Y J, ZHU X, LI J, LI M F, LIN Y C, HE J G, LIU L. Meroterpenes and azaphilones from marine mangrove endophytic fungus Penicillium 303#. Fitoterapia, 2014, 97: 241-246.
[25]	FENG Q M, YU Y, TANG M X, ZHANG T Y, ZHANG M Y, WANG H F, HAN Y Q, ZHANG Y X, CHEN G, PEI Y H. Four new hybrid polyketide-terpenoid metabolites from the Penicillium sp. SYPF 7381 in the rhizosphere soil of Pulsatilla chinensis. Fitoterapia, 2018, 125: 249-257.
[26]	MATSUDA Y, AWAKAWA T, WAKIMOTO T, ABE I. Spiro-ring formation is catalyzed by a multifunctional dioxygenase in austinol biosynthesis. Journal of the American Chemical Society, 2013, 135(30): 10962-10965.
[27]	STIERLE D B, STIERLE A A, PATACINI B. The berkeleyacetals, three meroterpenes from a deep water acid mine waste Penicillium. Journal of Natural Products, 2007, 70(11): 1820-1823.
[28]	时杰, 余玥, 刘冰, 李文兰. 真菌来源杂萜类化合物的研究进展. 中国药学杂志, 2024, 59(6): 476-485.
	SHI J, YU Y, LIU B, LI W L. Research progress of meroterpenoids produced by fungi. Chinese Pharmaceutical Journal, 2024, 59(6): 476-485. (in Chinese)
[29]	韩海燕. 大型真菌中含苔色酸核心骨架杂萜类化合物的生物合成研究[D]. 哈尔滨: 东北林业大学, 2024.
	HAN H Y. Biosynthesis of meroterpenoids containing an orsellinic acid backbone in macrofungi[D]. Harbin: Northeast Forestry University, 2024. (in Chinese)
[30]	龙涛. 一类DMOA衍生杂萜核心骨架的合成[D]. 贵阳: 贵州大学, 2024.
	LONG T. Synthesis of a class of DMOA-derived hybrid terpenoid core skeletons[D]. Guiyang: Guizhou University, 2024. (in Chinese)
[31]	GAN D, LIU J Q, YANG Y J, WANG C Y, ZHU L, LI C Z, CAI L, DING Z T. Phytotoxic meroterpenoids with herbicidal activities from the phytopathogenic fungus Pseudopestalotiopsis theae. Phytochemistry, 2023, 206: 113522.
[32]	DARMA R, SHANG Z, BRACEGIRDLE J, MOGGACH S, MCDONALD M C, PIGGOTT A M, SOLOMON P S, CHOOI Y H. Transcriptomics-driven discovery of new meroterpenoid rhynchospenes involved in the virulence of the barley pathogen Rhynchosporium commune. ACS Chemical Biology, 2025, 20(2): 421-431.
[33]	HUANG Z H, LIANG X, LI C J, GU Q, MA X, QI S H. Talaromynoids A-I, highly oxygenated meroterpenoids from the marine-derived fungus Talaromyces purpureogenus SCSIO 41517 and their lipid accumulation inhibitory activities. Journal of Natural Products, 2021, 84(10): 2727-2737.
[34]	WU Y Z, XIA G Y, XIA H, WANG L Y, WANG Y N, LI L, SHANG H C, LIN S. Seco and nor-seco isodhilarane-type meroterpenoids from Penicillium purpurogenum and the configuration revisions of related compounds. Journal of Natural Products, 2022, 85(1): 248-255.
[35]	WANG X D, LI N, ZAN T X, XU K, GAO S H, YIN Y X, YAO M H, WANG F. Genome-wide analysis of the TIFY family and function of CaTIFY7 and CaTIFY10b under cold stress in pepper (Capsicum annuum L.). Frontiers in Plant Science, 2023, 14: 1308721.
[36]	PAN J J, HU Y R, WANG H P, GUO Q, CHEN Y N, HOWE G A, YU D Q. Molecular mechanism underlying the synergetic effect of jasmonate on abscisic acid signaling during seed germination in Arabidopsis. The Plant Cell, 2020, 32(12): 3846-3865.
[37]	PELAGIO-FLORES R, ORTIZ-CASTRO R, MENDEZ-BRAVO A, MACIAS-RODRIGUEZ L, LOPEZ-BUCIO J. Serotonin, a tryptophan-derived signal conserved in plants and animals, regulates root system architecture probably acting as a natural auxin inhibitor in Arabidopsis thaliana. Plant and Cell Physiology, 2011, 52(3): 490-508.
[38]	WAN J P, ZHANG P, SUN L L, LI S, WANG R L, ZHOU H K, WANG W Y, XU J. Involvement of reactive oxygen species and auxin in serotonin-induced inhibition of primary root elongation. Journal of Plant Physiology, 2018, 229: 89-99.
[39]	PAN J J, WANG H P, YOU Q G, CAO R, SUN G L, YU D Q. Jasmonate-regulated seed germination and crosstalk with other phytohormones. Journal of Experimental Botany, 2023, 74(4): 1162-1175.

引物名称Primer name	引物序列Primer sequence
JAZ-F	TGCCGATAGCAAGGAGGAAT
JAZ-R	ATTTCTCACCGTCTGCTTGC
EC4.1.1.28 (1)-F	GGGTCTCGTGCCTACTTACA
EC4.1.1.28 (1)-R	GCATTGAACATGGCAGCAAC
EC4.1.1.28 (2)-F	TGAAGCCCACCGTGTTCAT
EC4.1.1.28 (2)-R	AGCCCTAACTTCTTCGCCTT
EC2.1.1.4-F	GGCACTCAAGTGTGCAGTAG
EC2.1.1.4-R	TTCAGAAGCCTCTGCACGTA
Actin-F	TCCCTGGTATCGCTGACCGTA
Actin-R	CCCTTTGAGATCCACATCTGTT

指标 Index	化合物 Compound	供测浓度Test concentration (μg·mL^-1)						EC₅₀(μg·mL^-1)
指标 Index	化合物 Compound	10	20	30	40	50	60	EC₅₀(μg·mL^-1)
胚根抑制率 Inhibition rate for radicle (%)	草甘膦Glyphosate	26.0±0.6b	41.5±0b	48.9±0c	57.9±2.3c	60.7±0.4c	61.7±7.0c	30.9
	乙草胺Acetochlor	74.6±1.0a	78.1±1.3a	79.0±0.9b	79.5±1.9b	80.7±1.8b	81.6±0.8b	<10.0
	3	16.7±3.6c	21.0±2.2c	23.2±1.8d	24.1±1.7e	28.1±2.0e	48.7±3.5d	107.9
	4	9.0±1.4d	10.6±0.1d	19.0±2.1e	30.0±4.2d	34.2±1.4d	36.2±1.5e	89.3
	6	23.7±2.8b	42.0±3.2b	83.7±0.6a	87.1±1.0a	91.2±0.7a	92.9±0.7a	19.1
胚芽抑制率 Inhibition rate for germ (%)	草甘膦Glyphosate	21.0±3.2c	25.0±0c	39.3±1.9c	41.6±4.4c	44.7±8.1b	47.1±5.2b	66.2
	乙草胺Acetochlor	79.2±0.6a	81.5±0.4a	83.0±0.1a	84.4±0.7a	85.1±1.0a	86.3±1.7a	<10.0
	3	7.7±1.0d	9.6±1.2d	10.4±0.4d	19.5±2.3d	23.3±0.6c	37.2±5.3c	89.6
	4	1.8±0.5e	2.1±1.1e	6.7±0.3e	9.8±0.5e	14.6±2.2d	25.3±3.1d	94.5
	6	27.4±3.0b	55.8±3.6b	73.3±1.2b	75.1±1.2b	78.1±2.0a	80.1±0.7a	17.9


样品 Sample	原始测序数据总条目数 Raw reads	原始测序总数据量 Raw bases	质控后测序数据总条目数 Clean reads	质控后测序总数据量 Clean bases	错误率 Error rate (%)	Q30百分比 Q30 (%)	GC碱基含量 GC content (%)
CK_1	45487752	6868650552	45141502	6763367603	0.0127	94.76	50.55
CK_2	52780488	7969853688	52378850	7852792340	0.0126	94.90	49.58
CK_3	43547122	6575615422	43203412	6480763587	0.0127	94.80	49.92
6_1	42260856	6381389256	41909208	6285904222	0.0129	94.52	50.60
6_2	44006146	6644928046	43647696	6548403658	0.0129	94.38	50.51
6_3	46373380	7002380380	46009254	6903393150	0.0125	95.09	50.70

菌核青霉HY5中3,5-二甲基苔色酸类混源萜的结构及其除草活性

Structure and Herbicidal Activity of 3,5-Dimethylorsellinic Acid-Derived Meroterpenoids from Penicillium sclerotiorum HY5

RichHTML

PDF

赞

可视化

摘要/Abstract

引用本文

使用本文

图/表 8

参考文献 39

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	李睿, 梁跃, 白杨, 张桂悦, 王楠楠, 乔松林, 张改平. 自噬在猪繁殖与呼吸综合征病毒感染中的作用机制研究进展[J]. 中国农业科学, 2025, 58(4): 792-801.
[2]	陈志,张逸,路钦越,郭佳禾,梁艳,张明怡星,杨章平. 茶树油对LPS诱导的奶牛乳腺炎的作用及其机制[J]. 中国农业科学, 2021, 54(14): 3124-3133.
[3]	付兵,王美,刘建阳,林伟,张成省,赵栋霖. 海洋来源杂色曲霉次级代谢产物及其抗植物病原细菌活性[J]. 中国农业科学, 2020, 53(19): 3964-3974.
[4]	马树杰，刘琳，芦小鹏，马志卿，张兴. 中国粗榧生物碱的除草活性[J]. 中国农业科学, 2016, 49(19): 3746-3753.
[5]	曾幼玲，杨瑞瑞. 植物miRNA的生物学特性及在环境胁迫中的作用[J]. 中国农业科学, 2016, 49(19): 3671-3682.
[6]	张量，张敬泽. 渐绿木霉抑菌物质的分离纯化及其对植物病原菌的抑制作用[J]. 中国农业科学, 2015, 48(5): 882-888.
[7]	钟守琴，刘波，魏朝富，胡斐南. 紫色泥岩土壤＜2 mm岩屑及其对抗剪强度的作用机制[J]. 中国农业科学, 2015, 48(23): 4846-4858.
[8]	高芬12, 吴元华2, 王梦亮1. 可可链霉菌182-2活性代谢产物对烟草赤星病菌的作用[J]. 中国农业科学, 2013, 46(23): 4933-4940.
[9]	卢彩鸽, 刘伟成?, 刘霆, 董丹, 张涛涛, 刘德文. 利迪链霉菌A01活性代谢产物对甘蓝枯萎病菌的抑制作用及其机理[J]. 中国农业科学, 2012, 45(18): 3764-3772.
[10]	张利辉, 冯江兰, 董金皋. 灰葡萄孢BC7-3菌株固体发酵条件的优化[J]. 中国农业科学, 2011, 44(16): 3477-3484.
[11]	何璐, 纪明山, 王勇, 徐洪波, 齐补坤. 一株苦参内生真菌的抑菌特性及活性成分的结构鉴定[J]. 中国农业科学, 2011, 44(15): 3127-3133 .
[12]	孙卫青,周光宏,徐幸莲,彭增起 . 蒸煮腌肉色素的结构分析[J]. 中国农业科学, 2010, 43(9): 1912-1918 .
[13]	滕云,余志义,崔文华,张新刚,全鑫,孙秋,侯太平 . 高山绣线菊提取物对几种植物真菌的抑制活性及其活性成分[J]. 中国农业科学, 2009, 42(7): 2380-2385 .
[14]	徐娇,许文超,康占海,刘保峰,张金林. 瓜果腐霉除草活性成分的分离鉴定[J]. 中国农业科学, 2009, 42(6): 1994-2001 .
[15]	. 万隆霉素抗菌活性成分的分离鉴定[J]. 中国农业科学, 2008, 41(10): 3104-3109 .