红皮白肉与红皮红肉火龙果植株茎部内生微生物群落结构特征

doi:10.3864/j.issn.0578-1752.2024.02.015

摘要/Abstract

摘要：

【目的】比较分析红皮白肉与红皮红肉火龙果植株茎部内生微生物群落结构特征，旨在探究红肉火龙果果肉颜色形成与植株内生微生物群落的关联性，挖掘与其颜色形成相关的功能微生物。【方法】以红皮白肉与红皮红肉火龙果植株茎部为试验材料，基于高通量测序技术比较分析茎部内生微生物（细菌、真菌）多样性与丰富度，门、属分类水平的群落结构组成以及优势菌群相对丰度占比；基于LEfSe差异分析探究红皮白肉与红肉火龙果植株茎部中具有显著差异的内生微生物。【结果】红皮白肉与红皮红肉火龙果植株茎部内生微生物群落结构特征存在显著差异。红皮红肉火龙果植株茎部中特有的细菌、真菌OTU数量均高于相应的白肉火龙果；门分类水平表现为，子囊菌门（Ascomycota）真菌在红肉火龙果植株茎部中的相对丰度占比高于相应的白肉火龙果，其相对丰度占比是白肉火龙果的1.15倍；属分类水平表现为，链霉菌属（Streptomyces）细菌是红肉火龙果植株茎部中相对丰度占比最高的优势细菌属，青霉菌属（Penicillium）真菌是红肉火龙果植株茎部中相对丰度占比最高的优势真菌属，二者相对丰度占比分别是白肉火龙果的1.24倍和4.27倍；另一方面，红肉火龙果植株茎部中还富集了列契瓦尼尔氏菌属（Lechevalieria）、糖霉菌属（Glycomyces）、未分类肠杆菌属（unclassified_f__Enterobacteriaceae）、马杜拉放线菌属（Actinomadura）等优势细菌属以及unclassified_f__Serendipitaceae、unclassified_c__GS13、unclassified_o__Atractiellales、unclassified_o__Auriculariales、篮状菌属（Talaromyces）等优势真菌属。LEfSe差异分析结果显示，原小单孢菌属（Promicromonospora）细菌和木霉菌属（Xylomyces）真菌在红肉火龙果植株茎部中显著富集。【结论】内生微生物群落组成与红肉火龙果果肉中色素的产生、代谢积累密切相关；链霉菌属、未分类肠杆菌属、原小单孢菌属细菌及子囊菌门、青霉菌属、篮状菌属和木霉菌属真菌可能是红肉火龙果果肉色素合成与代谢积累相关的功能微生物。

关键词: 火龙果, 果肉颜色, 内生微生物, 高通量测序

Abstract:

【Objective】 The differences of the endophytic microbial community structures between Hylocereus undatus and H. polyrhizus were analyzed, so as so to explore the correlation between the pulp color of H. polyrhizus and the endophytic microbial community composition and their functional microorganisms. 【Method】 Based on high-throughput sequencing technology, the diversity and richness of endophytic microorganisms (bacteria and fungi) in stems between H. undatus and H. polyrhizus were analyzed. Meanwhile, based on LEfSe analysis, the differences of endophytic microorganisms in stems between H. undatus and H. polyrhizus were also investigated.【Result】 The significant differences of the endophytic microbial community structures were found in stems between H. undatus and H. polyrhizus. Meanwhile, the numbers of specific bacterial and fungal OTUs in stems of H. polyrhizus were all higher than those of H. undatus. At the phylum level, the relative abundance ratio of Ascomycota in stems of H. polyrhizus was 1.15 times higher than that of H. undatus. At the genus level, Streptomyces and Penicillium were the highest abundant dominant bacterial and fungal genera in stems of H. polyrhizus, which were 1.24 and 4.27 times higher than those of H. undatus, respectively. In addition, some bacterial genera, such as Lechevalieria, Glycomyces, unclassified_f__Enterobacteriaceae, Actinomadura, and some fungal gerera, such as Talaromyces, unclassified_f__Serendipitaceae, unclassified_c__GS13, unclassified_o__Atractiellales, unclassified_o__Auriculariales were enriched in stems of H. polyrhizus. LEfSe analysis also showed that Promicromonospora and Xylomyces were significant enriched in stems of H. polyrhizus. 【Conclusion】 All above results suggested that the formation of pigment was closely related to the compositions of endophytic microbial community in stems of H. polyrhizus. The bacterial genera, such as Streptomyces, unclassified_f__Enterobacteriaceae, Promicromonospora, and the fungal phylum and genera, such as Ascomycota, Penicillium, Talaromyces and Xylomyces, were all the potential microorganisms in relating to pigment synthesis and metabolic accumulation in stems of H. polyrhizus.

Key words: Pitaya (Hylocereus undulatus Britt), pulp color, endophytic microorganisms, high-throughput sequencing

周信雁, 陈思宇, 魏宇飞, 朱瑜, 冯君茜, 丁点草, 陆贵锋, 杨尚东. 红皮白肉与红皮红肉火龙果植株茎部内生微生物群落结构特征[J]. 中国农业科学, 2024, 57(2): 416-428.

ZHOU XinYan, CHEN SiYu, WEI YuFei, ZHU Yu, FENG JunQian, DING DianCao, LU GuiFeng, YANG ShangDong. Characteristics of Endophytic Microbial Community Structures in Stems Between Hylocereus undatus and H. polyrhizus[J]. Scientia Agricultura Sinica, 2024, 57(2): 416-428.

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链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2024.02.015

https://www.chinaagrisci.com/CN/Y2024/V57/I2/416

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引物名称 Primer name	引物类型 Primer type	引物序列 Primer sequence (5′→3′)	测序平台 Sequencing platform	序列长度 Sequencing length (bp)
799F 1193R	内生细菌 Endophytic bacteria	AACMGGATTAGATACCCKG ACGTCATCCCCACCTTCC	MiSeq	394
ITS1F	内生真菌 Endophytic fungi	CTTGGTCATTTAGAGGAAGTAA		242
ITS2R	内生真菌 Endophytic fungi	GCTGCGTTCTTCATCGATGC		242

处理 Treatment		OTU数量 Numbers of operational taxonomic units	不同分类类别数量 Numbers of different taxonomic categories
处理 Treatment		OTU数量 Numbers of operational taxonomic units	门 Phylum	纲 Class	目 Order	科 Family	属 Genus	种 Species
内生细菌Endophytic bacteria	白肉RW	466	18	33	93	149	262	366
	红肉RR	491	19	35	95	157	274	377
	总计Total	555	21	40	106	174	303	424
内生真菌Endophytic fungi	白肉RW	268	6	20	44	88	125	160
	红肉RR	340	7	24	58	103	153	203
	总计Total	466	7	25	63	117	191	261

处理 Treatment		香农指数 Shannon index	辛普森指数 Simpson index	Ace指数 Ace index	Chao1指数 Chao1 index	覆盖范围 Coverage (%)
内生细菌 Endophytic bacteria	白肉RW	3.71±0.46a	0.07±0.032a	410.36±56.91a	403.63±63.72a	0.99
内生细菌 Endophytic bacteria	红肉RR	3.82±0.58a	0.06±0.032a	408.99±57.14a	404.74±63.53a	0.99
内生真菌 Endophytic fungi	白肉RW	1.84±0.93a	0.38±0.301a	154.52±49.35a	156.17±48.77a	0.99
内生真菌 Endophytic fungi	红肉RR	2.65±0.14a	0.13±0.008a	171.38±40.97a	175.96±38.94a	0.99