白菜硫代葡萄糖甙合成中MAM 的进化分析

doi:10.3864/j.issn.0578-1752.2014.21.016

摘要/Abstract

摘要： 【目的】探索硫代葡萄糖甙合成中关键基因-甲基硫代烷烃基苹果酸合成酶（methylthioalkylmalate，MAM）基因在白菜基因组中的进化情况，为研究白菜MAM的功能提供理论指导。【方法】根据白菜数据库BARD信息分析拟南芥、盐芥和白菜MAM的共线性关系，利用MEME在线软件预测MAM序列的高度保守基序（motif），通过R软件分析93份白菜材料MAM的表达模式，并利用MEGA5.05软件构建MAM的系统进化树。【结果】白菜的3个亚基因组均保留着MAM的同源基因。其中与拟南芥具有共线性的5个同源基因可能源自基因组古三倍化复制，其它两个同源基因可能是由转座扩增产生的；基序分析结果表明，与拟南芥的MAM相比，白菜的MAM同源基因存在基序的缺失，并且大多发生在序列的两端；在93份白菜材料中，白菜MAM同源基因之间的表达量有很大差异，Bra021947与Bra018524只在少数材料中存在极低的表达，并且Bra029356的表达量低于可检测水平；进化树分析发现，除Bra018524外，其他的白菜MAM同源基因与拟南芥的MAM聚集在不同的分支上。【结论】白菜的MAM可能在拟南芥和白菜物种分化之后独立起源并进化出不同的功能。

关键词: 白菜, 硫甙, 白菜MAM , 共线性, 进化树

Abstract: 【Objective】The objective of this study is to explore the duplication and evolution of Brassica rapa MAM which are key genes in the process of glucosinolates synthesis. This study will provide a theoretical basis for future MAM research. 【Method】 The synthenic relationship of MAM genes among Arabidopsis thaliana, Schrenkiella parvula and Brassica rapa were analyzed, according to B. rapa datebase BARD, conserved motif of MAM genes were predicted by online software MEME, the expression of MAM homologous genes in 93 materials of Brassica rapa was analyzed by software R, and the phylogenetic tree was constructed by software MEGA 5.05. 【Result】 The results showed that the MAM homologous genes were located in three subgenomes of B. rapa. Amongst seven MAM genes, five geneswhich are synthenic orthologs to A. thaliana mainly originated from whole genome triplication. The remaining two genes may derive from translocation. Motif analysis result indicated that there were many differences in both ends of protein sequences in BrMAM homologous genes, compared with AtMAM genes. Expression data showed that the expression level of these homologous genes was variable, Bra021947 and Bra018524 expressed little in several materials of B. rapa, the expression of Bra029356 could be detected in all materials. In the phylogenetic tree, BrMAM homologous genes and AtMAM genes were located in different branches, in addition to Bra018524. 【Conclusion】 These results indicated that the B. rapa MAM genes had an independent evolution after the divergence of B. rapa and A. thaliana.

Key words: Brassica rapa, glucosinolates, BrMAM genes, synteny, phylogenetic tree

刘锦，张冀芳，梁建丽，程锋，武剑，王晓武. 白菜硫代葡萄糖甙合成中MAM 的进化分析[J]. 中国农业科学, 2014, 47(21): 4309-4317.

LIU Jin, ZHANG Ji-fang, LIANG Jian-li, CHENG Feng, WU Jian, WANG Xiao-wu. Evolutionary Study of MAM Genes Which Function in the Process of Glucosinolates Synthesis in Brassica rapa[J]. Scientia Agricultura Sinica, 2014, 47(21): 4309-4317.

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