中国农业科学 ›› 2013, Vol. 46 ›› Issue (16): 3452-3459.doi: 10.3864/j.issn.0578-1752.2013.16.016

• 畜牧·兽医·资源昆虫 • 上一篇    下一篇

黑色素细胞中过量表达miR-137对TYRP-1和TYRP-2的影响

马淑慧1, 薛霖莉2, 徐刚1, 侯亚琴3, 耿建军1, 曹靖4, 赫晓燕1, 王海东1, 董常生1   

  1. 1.山西农业大学动物科技学院,山西太谷 030801
    2.山西农业大学信息学院,山西太谷 030801
    3.山西省长治市畜牧兽医局,山西长治 046000
    4.北京农业职业学院畜牧兽医系,北京 102442
  • 收稿日期:2013-03-26 出版日期:2013-08-15 发布日期:2013-05-27
  • 通讯作者: 通信作者王海东,Tel:0354-6288980;E-mail:13623541993@163.com
  • 作者简介:马淑慧,E-mail:mashuhui880113@163.com
  • 基金资助:

    山西省青年基金项目(2012021028-3)、国家自然科学基金项目(30671512、31172283)

The Influences of Over-Expressing miR-137 on TYRP-1 and TYRP-2 in Melanocytes

MA Shu-hui1, XUE Lin-li2, XU Gang1, HOU Ya-qin3, GENG Jian-jun1, CAO Jing4, HE Xiao-yan1, WANG Hai-dong1, DONG Chang-sheng1   

  1. 1.College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801
    2.College of Information, Shanxi Agricultural University, Taigu 030801
    3.Animal Husbandry and Veterinary Bureau of Shanxi Province,Changzhi 046000
    4.Beijing Vocational College of Agriculture Department of Animal Husbandry and Veterinary, Beijing 102442
  • Received:2013-03-26 Online:2013-08-15 Published:2013-05-27

摘要: 【目的】证明miR-137通过调控MITF而间接影响小鼠黑色素细胞TYRP-1和TYRP-2的表达,从而影响黑色素的生成。【方法】通过细胞转染技术在细胞水平过量表达miR-137,对黑色素细胞中黑色素含量进行了测定,经RT-PCR以及western blot检测转染后细胞TYRP-1和TYRP-2在mRNA和蛋白水平的变化。【结果】黑色素含量明显减少,在mRNA水平,TYRP1显著降低至0.4倍,TYRP-2显著降低至0.6倍。在蛋白水平,TYRP-1显著降低至0.61倍,TYRP-2显著降低至0.73倍。【结论】过量表达miR-137会通过调控MITF而使黑色素细胞TYRP-1和TYRP-2的表达量降低,从而揭示了miR-137通过调节TYRP-1和TYRP-2的表达间接调控了黑色素的生成。

关键词: miR-137 , MITF , TYRP-1 , TYRP-2 , 黑色素

Abstract: 【Objective】Coat color is one of the most important economic characters. There are as many as hundreds of regulation genes involved in the formation process of coat color, and the mechanism(s) have not been completely determined. TYRP-1 and TYRP-2 are involved in regulation and control of melanin synthesis, and they are members of tyrosinase family. MITF is inhibited by miR-137 at the translational level, however, TYRP-1 and TYRP-2 were regulated by MITF at the transcriptional level. In this study, the effect of miR-137 on the production of TYRP-1 and TYRP-2 by regulating MITF was studied. 【Method】Over-expression of miR-137 was conducted through the cell transfection technique and the contents of melanin in melanocytes were extracted, and then the level of TYRP-1 and TYRP-2 was detected using RT-PCR and western blot. 【Result】 The results showed that the contents of melanin were significantly reduced. TYRP1 mRNA was significantly reduced to 0.4 times and TYRP-2 mRNA was significantly reduced to 0.6 times. TYRP-1 protein was significantly reduced to 0.61 times and TYRP-2 protein was significantly reduced to 0.73 times. 【Conclusion】 Results of the study suggested that miR-137 indirectly regulates the variation of YRP-1 and TYRP-2 by regulating MITF, and the miR-137 may indirectly mediate the alteration of coat color through regulating the expression of TYRP-1and TYRP-2.

Key words: miR-137 , MITF , TYRP-1 , TYRP-2 , melanin