胆碱对鹅体内脂质代谢及肝脏FAS基因mRNA表达的影响

doi:10.3864/j.issn.0578-1752.2013.13.015

摘要/Abstract

摘要： 【目的】探讨胆碱对青农灰鹅体内脂质转运、代谢的作用及脂肪酸合成酶FAS（fatty acid synthase）基因表达的影响，确定鹅日粮中胆碱的最适添加量。【方法】将180只1日龄青农灰鹅随机分为6个处理组，每处理组3个重复，每个重复10只。试验在玉米-豆粕型饲粮的基础上各组的胆碱添加量分别为0、600、1 200、1 800、2 400和3 000 mg•kg-1，试验期15周。屠宰后测定肝脏和血清总胆固醇、甘油三酯，血清低密度脂蛋白胆固醇、高密度脂蛋白胆固醇、葡萄糖、胰岛素、胰高血糖素水平，肝脏脂蛋白酯酶、肝脂酶及血清胆碱酯酶活性和肝脏脂肪酸合成酶（FAS）基因表达量。【结果】①4周龄时，饲粮中添加胆碱显著降低肝脏和血清总胆固醇、甘油三酯、血清低密度脂蛋白胆固醇、胰岛素水平和血清胆碱酯酶活性（P＜0.05或P＜0.01）；显著提高肝脏脂蛋白酯酶、肝脂酶活性和血清胰高血糖素水平（P＜0.05）；但对血清高密度脂蛋白胆固醇、葡萄糖含量影响不显著（P＞0.05）。15周龄时，饲粮中添加胆碱显著降低肝脏和血清总胆固醇、甘油三酯、低密度脂蛋白胆固醇含量与胰岛素水平及血清胆碱酯酶活性（P＜0.05或P＜0.01）；显著提高肝脏脂蛋白酯酶、肝脂酶活性和血清高密度脂蛋白胆固醇、胰高血糖素水平（P＜0.05）；但对血清葡萄糖含量影响不显著（P＞0.05）。②饲粮中添加胆碱显著提高肝脏FAS基因的表达量（P＜0.01），且肝脏脂肪酸合成酶基因表达量随胆碱添加水平呈先下降后上升趋势。【结论】综合考虑胆碱对鹅肝脏脂质代谢的影响，1—4和5—15周龄鹅饲粮中胆碱适宜添加量分别为1 200—1 800和1 200 mg•kg-1。

关键词: 胆碱 , 青农灰鹅 , 肝脂、血液生化及激素指标 , FAS基因

Abstract: 【Objective】 This experiment was conducted to study the effects of choline supplemented in diet on lipids metabolism and FAS mRNA expression in liver tissue of Qingnonghui geese. 【Method】 One hundred and eighty Qingnonghui geese at 1 day of age were selected and randomly divided into 6 groups with 3 replicates for each group and 10 geese for each replicate. Six groups of geese were fed the diets supplemented with 0, 600, 1 200, 1 800, 2 400 and 3 000 mg•kg-1 choline in the basal diets, respectively. The experiment lasted for fifteen weeks. The contents of liver and serum total cholesterol, triglyceride, serum low density lipoprotein cholesterol, high density lipoprotein cholesterol, blood glucose, insulin and glucagon level, liver lipoprotein lipase, hepatic lipase and serum cholinesterase activities, the gene expression of fatty acid synthetase mRNA were measured.【Result】 The results showed that at 4 weeks of age, supplementation of choline in diet significantly decreased the contents of liver and serum total cholesterol, triglyceride, serum low density lipoprotein cholesterol, insulin level, and serum cholinesterase activity (P＜0.05 or P＜0.01), and significantly increased the contents of serum glucagon and the activity of liver lipoprotein lipase and hepatic lipase (P＜0.05), but had no significant difference in contents of serum high density lipoprotein cholesterol and blood glucose (P＞0.05). At 15 weeks of age, supplementation of choline in diet significantly decreased the contents of liver and serum total cholesterol, triglyceride, serum low density lipoprotein cholesterol, insulin level and serum cholinesterase activity (P＜0.05 or P＜0.01), and significantly increased the contents of serum high density lipoprotein cholesterol, glucagon and the activities of liver lipoprotein lipase and hepatic lipase (P＜0.05), but had no significant difference in the contents of blood glucose (P＞0.05). Supplementation of choline in diet significantly increased the gene expression of fatty acid synthetase mRNA (P＜0.01), and the trend of it was decrease first and then increase, but had no significant conic relation between them (R2＜0.700). 【Conclusion】Comprehensively considering the effects of choline on lipids metabolism in liver of geese, under this experimental conditions, the optimal dietary of choline in diet is recommended as 1 200-1 800 mg•kg-1 and 1 200 mg•kg-1 for 1-4 and 5-15 weeks of age geese.

Key words: choline , Qingnonghui goose , liver lipid and blood biochemical indexes , serum hormone , FAS gene

张文旭, 王宝维, 葛文华, 张名爱, 李文立. 胆碱对鹅体内脂质代谢及肝脏FAS基因mRNA表达的影响[J]. 中国农业科学, 2013, 46(13): 2777-2787.

ZHANG Wen-Xu, WANG Bao-Wei, GE Wen-Hua, ZHANG Ming-Ai, LI Wen-Li. Effects of Choline on Lipids Metabolism and FAS mRNA Expression in Liver of Qingnonghui Geese[J]. Scientia Agricultura Sinica, 2013, 46(13): 2777-2787.

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