细胞型朊蛋白在BV2小神经胶质细胞体外激活中的作用

doi:10.3864/j.issn.0578-1752.2013.09.021

中国农业科学 ›› 2013, Vol. 46 ›› Issue (9): 1932-1938.doi: 10.3864/j.issn.0578-1752.2013.09.021

细胞型朊蛋白在BV2小神经胶质细胞体外激活中的作用

付永瑶, 师福山, 王继宏, 杨利峰, 周向梅, 尹晓敏, 赵德明

中国农业大学动物医学院/国家动物海绵状脑病实验室，北京100193

收稿日期:2012-01-07 出版日期:2013-05-01 发布日期:2013-03-30
通讯作者: 通信作者赵德明，Tel：010-62732975；E-mail：zhaodm@cau.edu.cn
作者简介:付永瑶，Tel：010-62736980；E-mail：fyyabu@126.com
基金资助:
国家自然科学基金（31172293）、国家科技支撑计划（2011BAI15B01）

Effects of Prion Protein on the Regulation of Classical and Alternative Activation of BV2 Microglia in vitro

FU Yong-Yao, SHI Fu-Shan, WANG Ji-Hong, YANG Li-Feng, ZHOU Xiang-Mei, YIN Xiao-Min, ZHAO De-Ming

Laboratory of National Animal TSE/College of Veterinary Medicine, China Agricultural University, Beijing 100193

Received:2012-01-07 Online:2013-05-01 Published:2013-03-30

摘要/Abstract

摘要： 【目的】研究细胞型朊蛋白对小神经胶质细胞不同激活方式的影响。【方法】用IFN-γ、IL-4和IL-10分别刺激BV2细胞，RT-PCR方法检测PrPC的mRNA表达量。SiRNA干扰将PrPC沉默，用上述因子刺激细胞，用RT-PCR和Western-blot检测相关参数。【结果】用IFN-γ、IL-4和IL-10分别刺激小神经胶质细胞后可导致PrPC的mRNA表达量下降；PrPC沉默后的小神经胶质细胞对IFN-γ刺激的反应应答减弱；PrPC沉默可以显著地改变由IL-4诱导的神经胶质细胞的激活表型；但是对IL-10诱导的小神经胶质细胞激活却没有影响。【结论】PrPC既能影响小神经胶质细胞从静止状态到激活状态的转换，也在小神经胶质细胞的经典激活和替代激活途径中发挥调节作用。

关键词: 小干扰RNA , 朊蛋白 , 小神经胶质细胞 , 激活

Abstract: 【Objective】 The purpose of this study is to investigate the effects of PrPC on various forms of microglial activation. 【Method】 BV2 microglia were treated, respectively, with IFN-γ, IL-4, or IL-10, and the mRNA expression of PRNP was examined by RT-PCR. Then the effects of si-RNA-mediated disruption of PRNP on different parameters of microglial activation in IFN-γ, IL-4, or IL-10-stimulated microglia were analyzed by RT-PCR and western-blot. 【Result】PRNP mRNA expression was invariably downregulated in microglia upon exposure to IFN-γ, IL-4, or IL-10. PRNP silencing prior to cytokines treatment reduced the responsiveness of microglia to INF-γ treatment, significantly altered IL-4-induced microglial activation phenotype, and had no effect on IL-10-induced microglial activation.【Conclusion】Together, these results support a role of PrPC in the modulation of the shift of microglia from a quiescent state to an activated phenotype and in the regulation of the microglial response during classical and alternative activation.

Key words: SiRNA , prion protein , microglia , activation

付永瑶, 师福山, 王继宏, 杨利峰, 周向梅, 尹晓敏, 赵德明. 细胞型朊蛋白在BV2小神经胶质细胞体外激活中的作用[J]. 中国农业科学, 2013, 46(9): 1932-1938.

FU Yong-Yao, SHI Fu-Shan, WANG Ji-Hong, YANG Li-Feng, ZHOU Xiang-Mei, YIN Xiao-Min, ZHAO De-Ming. Effects of Prion Protein on the Regulation of Classical and Alternative Activation of BV2 Microglia in vitro[J]. Scientia Agricultura Sinica, 2013, 46(9): 1932-1938.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2013.09.021

https://www.chinaagrisci.com/CN/Y2013/V46/I9/1932

参考文献

[1]Aguzzi A, Sigurdson C, Heikenwaelder M. Molecular mechanisms of prion pathogenesis. Annual Review of Pathology-mechanisms of Disease, 2008, 3: 11-40.

[2]Linden R, Martins V R, Prado M A, Cammarota M, Izquierdo I, Brentani R R. Physiology of the prion protein. Physiological Reviews, 2008, 88(2): 673-728.

[3]Wu G. Nakajima K, Takeyama N, Yukawa M, Taniuchi Y, Sakudo A, Onodera T. Species-specific anti-apoptotic activity of cellular prion protein in a mouse PrP-deficient neuronal cell line transfected with mouse, hamster, and bovine Prnp. Neuroscience Letters, 2008, 446(1): 11-15.

[4]Prusiner S B. Prions. Proceedings of the National Academy of Sciences of the USA, 1998, 95(23): 13363.

[5]Nimmerjahn A, Kirchhoff F, Helmchen F. Resting microglial cells are highly dynamic surveillants of brain parenchyma in vivo. Science, 2005, 308(5726): 1314-1318.

[6]Soulet D, Rivest S. Bone-marrow-derived microglia: myth or reality? Current Opinion in Pharmacology, 2008, 8(4): 508-518.

[7]Garden G A, Möller T. Microglia biology in health and disease. Journal of Neuroimmune Pharmacology, 2006, 1(2): 127-137.

[8]Ransohoff R M, Perry V H. Microglial physiology: unique stimuli, specialized responses. Annual Review of Immunology, 2009, 27: 119-145.

[9]Colton C A. Heterogeneity of microglial activation in the innate immune response in the brain. Journal of Neuroimmune Pharmacology, 2009, 4(4): 399-418.

[10]Mosser D M. The many faces of macrophage activation. Journal of Leukocyte Biology, 2003, 73(2): 209-212.

[11]Latz E. The inflammasomes: mechanisms of activation and function. Current Opinion in Immunology, 2010, 22(1): 28-33.

[12]Martinez F O, Helming L, Gordon S. Alternative activation of macrophages: an immunologic functional perspective. Annual Review of Immunology, 2009, 27: 451-483.

[13]Henn A, Lund S, Hedtjärn M, Schrattenholz , Pörzgen P, Leist M. The suitability of BV2 cells as alternative model system for primary microglia cultures or for animal experiments examining brain inflammation. Altex, 2009, 26(2): 83.

[14]Wong M L, Medrano J F. Real-time PCR for mRNA quantitation. Biotechniques, 2005, 39(1): 75.

[15]Gordon S. Alternative activation of macrophages. Nature Reviews Immunology, 2003, 3(1): 23-35.

[16]Davis B K, Wen H, Ting J P Y. The inflammasome NLRs in immunity, inflammation, and associated diseases. Annual Review of Immunology, 2011, 29: 707-735.

[17]Franchi L, Eigenbrod T, Muñoz-Planillo R, Nuñez G. The inflammasome: a caspase-1-activation platform that regulates immune responses and disease pathogenesis. Nature Immunology, 2009, 10(3): 241-247.

[18]Martinon F, Mayor A, Tschopp J. The inflammasomes: guardians of the body. Annual Review of Immunology, 2009, 27: 229-265.

[19]Schroder K, Tschopp J. The inflammasomes. Cell, 2010, 140(6): 821-832.

[20]Hume D A, Ross I L, Himes S R, Sasmono R T, Wells C A, Ravasi T. The mononuclear phagocyte system revisited. Journal of Leukocyte Biology, 2002, 72(4): 621-627.

[21]Nguyen M D, Julien J P, Rivest S. Innate immunity: the missing link in neuroprotection and neurodegeneration? Nature Reviews Neuroscience, 2002, 3(3): 216-227.

[22]Adams D O, Hamilton T A. The cell biology of macrophage activation. Annual Review of Immunology, 1984, 2(1): 283-318.

[23]Colton C A, Mott R T, Sharpe H, Xu Q, Van Nostrand W E, Vitek M P. Expression profiles for macrophage alternative activation genes in AD and in mouse models of AD. Journal of Neuroinflammation, 2006, 3(1): 27.

细胞型朊蛋白在BV2小神经胶质细胞体外激活中的作用

Effects of Prion Protein on the Regulation of Classical and Alternative Activation of BV2 Microglia in vitro

PDF

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	张丹丹,许腾腾,高迪,齐昕,宁伟,汝振远,张翔栋,郭腾龙,申屠璐燕,于童,马洋洋,李运生,张运海,曹祖兵. 转录因子TEAD4对猪早期胚胎发育的调控[J]. 中国农业科学, 2021, 54(20): 4456-4465.
[2]	张宇飞,曹满园,王丽英,赵伟刚,李晓霞,常彤,许保增. 梅花鹿激活素A重组蛋白真核表达、纯化及其生物活性的测定[J]. 中国农业科学, 2020, 53(5): 1058-1070.
[3]	许冬,王玲,丛胜波,王金涛,李文静,万鹏. 红铃虫性信息素合成激活肽基因克隆、序列特征及在不同发育阶段的表达分析[J]. 中国农业科学, 2018, 51(23): 4449-4458.
[4]	李月，刘晓东，谢宗铭，董永梅，武冬梅，陈受宜. 棉花GhGT-2转录因子的克隆及其功能分析[J]. 中国农业科学, 2015, 48(24): 4872-4884.
[5]	许玲，卫培培，张大勇，徐照龙，何晓兰，黄益洪，马鸿翔，邵宏波. 大豆转录因子基因GmMYB111的克隆及功能分析[J]. 中国农业科学, 2015, 48(15): 3079-3089.
[6]	魏秀丽，谢小雷，张春晖，李侠，王春青. 猪宰后肌肉体系中μ-calpain及肌原纤维蛋白理化特性的变化规律[J]. 中国农业科学, 2015, 48(12): 2428-2438.
[7]	王文静, 马浩然, 李加纳, 张洪博. Ac/Ds转座子激活标签技术研究进展[J]. 中国农业科学, 2013, 46(14): 2845-2855.
[8]	王学斌, 俞慧清, 吴晓东, 徐旭俊, 陈建泉, 成国祥. 利用PRNP-/-羊制备朊蛋白多克隆抗体及其特性分析[J]. 中国农业科学, 2012, 45(7): 1399-1405.
[9]	张婷婷, 辛颖, 张志强, 任充华, 杨涵江, 王令, 张智英. PiggyBac转座子介导的诱导细胞永生化转基因载体的构建及其整合效率的检测[J]. 中国农业科学, 2012, 45(17): 3576-3583.
[10]	. 猪早期孤雌胚组蛋白乙酰化与组蛋白去乙酰化酶基因mRNA表达水平相关性研究 [J]. 中国农业科学, 2011, 44(6): 1224-1231 .
[11]	付大波,王友炜,侯永清,丁斌鹰,王蕾,刘玉兰,朱慧玲 . α-酮戊二酸对脂多糖刺激断奶仔猪肌肉能量代谢的影响 [J]. 中国农业科学, 2011, 44(4): 814-822 .
[12]	黄明, 黄峰, 黄继超, 徐宝才, 周光宏, 徐幸莲. 内源性蛋白酶对宰后肌肉嫩化机制研究进展[J]. 中国农业科学, 2011, 44(15): 3214-3222.
[13]	黄明;薛梅;黄继超;徐宝才 ;郭琳;徐幸莲;周光宏 . 鸡肉钙激活酶与其它畜禽肉中钙激活酶对钙离子浓度敏感性的比较[J]. 中国农业科学, 2011, 44(12): 2538-2544 .
[14]	张曼玲,赵丽华,周鑫,李荣凤 . 不同激活方法对猪孤雌胚胎单倍体率的影响 [J]. 中国农业科学, 2011, 44(1): 218-224 .
[15]	黄明,汤晓燕,黄峰,薛梅,周光宏 . 黄牛肉中钙激活酶系统的动力学性质#br# [J]. 中国农业科学, 2010, 43(8): 1664-1669 .