不同品种百合花挥发性成分定性与定量分析

doi:10.3864/j.issn.0578-1752.2013.04.013

摘要/Abstract

摘要： 【目的】研究不同品种百合（Lilium spp.）花挥发物的组成成分与释放量，寻找百合关键致香成分。【方法】采用活体植株动态顶空套袋-吸附采集法与ATD-GC/MS（自动热脱附-气质联用）分析技术，对4个杂种系7个品种百合花的挥发性成分进行定性与定量分析。【结果】试验共检测出64种化合物，属淡香型的亚洲百合和LA百合杂种系的3个品种含有39种，属浓香型的东方百合和麝香百合杂种系的4个品种含有54种。不同香型百合花的挥发性成分存在显著差异，亚洲百合和LA百合杂种系烷烃类化合物释放量较高，主要成分为2-乙基-1-己醇、乙苯、邻二甲苯、2,2,4,6,6-五甲基庚烷、5-乙基-2,2,3-三甲基庚烷、3-甲基十一烷和2,2,6-三甲基癸烷。东方百合和麝香百合杂种系萜烯类释放量最高，β-月桂烯、罗勒烯、芳樟醇、2-乙基-1-己醇为其主要成分。【结论】3个淡香型百合品种中没有检测到萜烯类化合物和苯甲酸甲酯，而4个浓香型百合品种中萜烯类释放量最高，主要为β-罗勒烯和芳樟醇，可初步确定这两种化合物为百合花致香的关键成分。

关键词: 百合 , 动态顶空套袋-吸附采集法 , ATD-GC/MS , 花香成分

Abstract: 【Objective】The aim of this experiment was to analyze the floral volatile composition and emission, and find out the key aroma components of Lilium spp.【Method】Dynamic headspace collection and ATD-GC/MS (Auto Thermal Desorber-Gas Chromatography/Mass Spectrometry) were used to qualitatively and quantitatively analyze the floral volatile components of seven varieties of Lilium spp. from four lily hybrids. 【Result】A total of 64 floral volatile compounds were identified from Lilium spp, 39 compounds were identified from 3 varieties of Asiatic hybrids and Longiflorum×Asiatic hybrids with weak fragrance, 54 compounds from 4 varieties of Oriental hybrids and Longiflorum hybrids with strong fragrance. The results showed that the floral volatile components were significantly different among lily hybrids. The amount of hydrocarbons compounds were the highest in Asiatic hybrids and Longiflorum×Asiatic hybrids, the main volatile components were 2-ethyl-1-hexanol, ethylbenzene, o-xylene, 2,2,4,6,6-pentamethyl-heptane, 5-ethyl-2,2,3-trimethyl-heptane, 3-methyl-undecane and 2,2,6-trimethyl decane. But the main components released from the flowers of Oriental hybrids and Longiflorum hybrids were terpenes,including β-myrcene, β-ocimene, linalool and 2-ethyl-1-hexanol.【Conclusion】Terpenes and benzoic acid methyl ester were not detected from 3 lily varieties with weak aroma, while it was found that terpens were released with the highest amounts in 4 lily varieties with strong aroma. β-ocimene and linalool were the main terpenes and may be identified as key aroma components of lily flowers.

Key words: Lilium spp. , dynamic headspace collection , ATD-GC/MS , floral scent component

张辉秀, 胡增辉, 冷平生, 王文和, 徐芳, 赵静. 不同品种百合花挥发性成分定性与定量分析[J]. 中国农业科学, 2013, 46(4): 790-799.

ZHANG Hui-Xiu, HU Zeng-Hui, LENG Ping-Sheng, WANG Wen-He, XU Fang, ZHAO Jing. Qualitative and Quantitative Analysis of Floral Volatile Components from Different Varieties of Lilium spp.[J]. Scientia Agricultura Sinica, 2013, 46(4): 790-799.

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参考文献

[1]赵秀英, 张振杰, 张宏利, 汪佑民. 黄蔷薇花精油化学成分的研究.西北植物学报, 1994, 14(5): l54-156．

Zhao X Y, Zhang Z J, Zhang H L,Wang Y M. Studies on chemical compounds of the esesntila oil from flowers of Rosa hugonis Hemsl. Acta Botanica Boreali-Occidentalia Sinica, 1994,14(5): l54-156. (in Chinese)

[2]Effmert U, Grobe J, Röse USR, Ehrig F, Kägi, Piechulla B. Volatile complsition, emission pattern and localization of floral scent emission in Mirabilis jalapa (Nyctaginaceae). American Journal of Botany, 2005, 92: 2-12.

[3]金荷仙, 陈俊愉, 金幼菊. 南京不同类型梅花品种香气成分的比较研究. 园艺学报, 2005, 32(6): 1139.

Jin H X, Chen J Y, Jin Y J. Comparison of different cultivars of Prunus mume's major gas ingredients. Acta Horticulturae Sinica, 2005, 32(6): 1139. (in Chinese)

[4]范正琪, 李纪元, 田敏, 李辛雷, 倪穗. 三个山茶花种(品种)香气成分初探. 园艺学报, 2006, 33(3): 592-596.

Fan Z Q, Li J Y, Tian M, Li X L, Ni S. Preliminary studies of aromatic constituents among three species(Variation) of camellia. Acta Horticulturae Sinica, 2006, 33(3): 592-596. (in Chinese)

[5]曹慧, 李祖光, 沈德隆. 桂花品种香气成分的GC/MS指纹图谱研究. 园艺学报, 2009, 36(3): 391-398.

Cao H, Li Z G, Shen D L. GC/MS fingerprint analysis of Osmanthus fragrans Lour. in different varieties. Acta Horticulturae Sinica, 2009, 36 (3) : 391-398. ( in Chinese)

[6]彭红明. 中国兰花挥发及特征花香挥发性成分研究[D]. 北京. 中国林业科学研究院, 2009.

Peng H M. Studies on floral volatile characteristic constituents of Chinese orchids[D]. Beijing: Chinese Academy of Forestry, 2009.(in Chinese)

[7]Morinage S I, Kumano Y, Ota A, Yamaoka R, Sakai S. Day-night fluctuations in floral scent and their effects on reproductive success in Lilium auratum. Population Ecology, 2009, 51: 187-195.

[8]回瑞华, 侯冬岩, 李铁纯. GC/MS法分析百合花化学成分. 鞍山师范学院学报, 2003, 5(2) : 61-63.

Hui R H, Hou D Y, Li T C. Studies on chemical constituents from Lilium brownii F.E. Brown var. Viridulum Baker by GC/MS. Journal of Anshan Normal Universityurnal of Anshan Normal University, 2003, 5(2): 61-63. (in Chinese)

[9]张继, 马君义, 姚健, 杨永利, 赵小亮, 尚博林, 陶媚娜. 西伯利亚百合花挥发油化学成分的研究. 西北植物学报, 2003, 23(12): 2184-2187.

Zhang J, Ma J Y, Yao J, Yang Y L, Zhao X L, Shang B L, Tao M N. Studies on the composition of the volatile oil from the flowers of Lilium×siberia. Acta Botanica Boreali-Occidentalia Sinica, 2003, 23(12): 2184-2187. (in Chinese)

[10]张继, 赵小亮, 马君义, 孟鑫, 郭耀, 姚健, 曾家豫. 巴巴拉百合花的天然香气成分研究. 西北植物学报, 2005, 25(4): 786-790.

Zhang J, Zhao X L, Ma J Y, Meng X, Guo Y, Yao J, Zeng J Y. Chemical components in flower fragrance of Lilium ×barbaresco. Acta Botanica Boreali-Occidentalia Sinica, 2005, 25(4): 786-790. (in Chinese)

[11]沈文胜, 芮昶, 曹慧, 李祖光. 固相微萃取-气相色谱-质谱法分析香水百合的头香成分. 质谱学报, 2006, 27(增刊): 101-103.

Shen W S, Rui C, Cao H, Li Z G. Analysis of headspace volatiles emitted from fresh flowers of Lilium. spp by gas chromatography– mass spectrometry with solid–phase microextraction. Journal of Chinese Mass Spectrometry Society, 2006, 27 (Suppl.): 101-103. (in Chinese)

[12]范燕萍, 范丽琨. 不同类型百合花瓣挥发性香气成分分析. 中国园艺学会观赏园艺专业委员会2008年学术年会论文集. 哈尔滨: 中国园艺学会, 2008: 418-421.

Fan Y P, Fan L K. Analysis on the volatile components of different varieties of Lilium. Chinese Society of Horticulture, Ornamental Horticulture Professional Committee of the 2008 Annual Conference Proceedings[C]. Harbin: China Institute of Horticulture, 2008: 418-421. (in Chinese)

[13]刘灿明, 黄高山, 刘祥华. 超临界CO2萃取百合花挥发油的工艺研究. 天然产物研究与开发, 2005, 17(4):485-487.

Liu C M, Huang G S, Liu X H. Extraction of volatile oil from lilium brownii by supercritical CO2. Natural Product Research and Development, 2005, 17(4): 485-487. (in Chinese)

[14]Dudareva N, Pichersky E. Biochemical and molecular genetic aspects of floral scents. Plant Physiology, 2000, 122: 627-628.

[15]Raguso R A, Pichersky E. Floral volatiles from Clarkia breweri and C. concinna (Onagraceae): Recent evolution of floral scent and moth pollination. Plant Systematics and Evolution, 1995, 194: 55-67.

[16]Raguso R A, Pellmyr O. Dynamic headspace analysis of floral volatiles: a comparison of methods. Oikos, 1998, 81: 238-254.

[17]Pichersky E, Noel J P, Dudareva N. Biosynthesis of plant volatiles: nature’s diversity and ingenuity. Science, 2006, 311: 808-811.

[18]Chen F, Tholl D, D’Auria J C, Farooq A, Pichersky E, Gershenzonb J. Biosynthesis and emission of terpenoid volatiles from Arabidopsis flowers. Plant Cell, 2003, 15: 481-494.

[19]范燕萍, 王旭日, 余让才, 杨萍. 不同种姜花香气成分分析. 园艺学报, 2007, 34: 231-234.

Fan Y P, Wang X R, Yu R C, Yang P. Analysis on the aroma components in several species Hedychium. Acta Horticulturae Sinica, 2007, 34: 231-234. (in Chinese)

[20]张银华, 熊秀芳, 徐盈. 湖北栀子花挥发油的GC/MS分析. 武汉植物学研究, 1999, 17(1): 61-63．

Zhang Y H, Xiong X F, Xu Y. Analysis of Hubei volatile oil of gardenial flower by GC/MS. Journal of Wuhan Botanical Research, 1999, 17 (1): 61-63. (in Chinese)

[21]张丽霞, 王日为. 茉莉花香气研究进展. 福建茶叶, 1999(5): 4-7．

Zhang L X, Wang R W. The research progress in the fragrance of jasmine flower. Tea in Fujian, 1999(5): 4-7. (in Chinese)

[22]李瑞红, 范燕萍. 白姜花不同开花时期的香味组分及其变化.植物生理学通讯, 2007, 43(1): 176-182．

Li R H, Fan Y P. Changes in floral aroma constituents in Hedychium coronarium Koenig during different blooming stages. Plant Physiology Communications, 2007, 43(1): 176-182. (in Chinese)

[23]罗心毅. 小果蔷薇精油的化学成分. 云南植物研究, 1988, 10(4): 483-485.

Luo X Y. Studies on chemical constituents of Rosa cymosa. Yunnan Plant Research, 1988, 10(4): 483-485. (in Chinese)

[24]蒲自连, 梁健. 淡黄杜鹃植物挥发油化学成分的研究. 应用与环境生物学报, 1999, 5(4): 371-373.

Pu Z L, Liang J. Studies on chemical constituents of volatile oil of yellow azalea plants. Chinese Journal of Applied & Environment Biology, 1999, 5(4): 371-373. (in Chinese)

[25]张继, 马君义, 黄爱仑, 姚建, 杨永利. 千里香杜鹃挥发性成分的分析研究. 园艺学报, 2002, 29(4): 386-388.

Zhang J, Ma J Y, Huang A L,Yao J, Yang Y L. Studies on the essential constituent of Rhododendron thymifolium Maxim. Acta Horticulturae Sinica, 2002, 29(4): 386-388. (in Chinese)