新疆南疆猪繁殖与呼吸综合征病毒分子流行病学研究

doi:10.3864/j.issn.0578-1752.2012.20.019

摘要/Abstract

摘要： 【目的】确定新疆南疆猪养殖场是否存在PRRSV感染，以及感染的PRRSV毒株类型及基因特点，从而针对性的有效防制PRRS。【方法】设计7对引物，对疑似PRRS发病猪场组织样品进行RT-PCR、克隆、测序和序列分析。【结果】47例样品，35例PRRSV阳性，阳性率高达74.47%（35/47）；35株均为美洲型毒株，其中有10株属于高致病性PRRSV，占总检测样品的21.28%（10/47），占阳性样品的28.57%（10/35）；综合分析本研究采集样品猪养殖场不存在欧洲型毒株。【结论】新疆南疆存在美洲型PRRSV感染，且存在高致病性毒株。

关键词: 新疆南疆, 猪繁殖与呼吸综合征病毒, 分子流行病学

Abstract: 【Objective】The objective of the study is to determine whether the porcine reproductive and respiratory syndrome virus infection is present in pig farms of South Xinjiang, and to determine the types and gene characteristics of PRRSV strain, thus pertinency effective prevention of PRRS can be made. 【Method】Seven pairs of primers were designed, and by using RT-PCR, the cloning, sequencing and sequence analysis on suspected PRRS pig tissue samples were conducted. 【Result】 There were 35 PRRSV positive in 47 test samples. The positive rate was as high as 74.47% (35/47). The 35 strains were all American type strain. There were 10 strains belong to the highly pathogenic PRRSV, accounting for 21.28% of the total tested samples (10/47), accounted for 28.57% of the positive samples(10/35); According to the comprehensive analysis, no Europe type of strains were found in the samples collected from these pig farms in this study. 【Conclusion】The American type of PRRSV strain infection and highly pathogenic strains exist in South Xinjiang.

Key words: South Xinjiang, porcine reproductive and respiratory syndrome virus （PRRSV）, molecular epidemiology

赵丽, 刘永宏, 焦海宏, 张志峰, 刘俊峰, 陈兖州, 温雅琴, 刘博, 李江涛, 崔浩然, 程波, 张春光. 新疆南疆猪繁殖与呼吸综合征病毒分子流行病学研究[J]. 中国农业科学, 2012, 45(20): 4288-4299.

ZHAO Li, LIU Yong-Hong, JIAO Hai-Hong, ZHANG Zhi-Feng, LIU Jun-Feng, CHEN Yan-Zhou, WEN Ya-Qin, LIU Bo, LI Jiang-Tao, CUI Hao-Ran, CHENG Bo, ZHANG Chun-Guang. Molecular Epidemiology Study on Porcine Reproductive and Respiratory Syndrome Virus in South Xinjiang[J]. Scientia Agricultura Sinica, 2012, 45(20): 4288-4299.

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