高致病性PRRSV与PCV2共感染协同致病性研究

doi:10.3864/j.issn.0578-1752.2012.18.019

摘要/Abstract

摘要： 【目的】为阐明高致病性猪繁殖与呼吸综合征病毒（HP-PRRSV）和猪圆环病毒2型（PCV2）的协同致病作用，澄清两种病毒不同时间顺序共感染与其协同致病力之间的关系。【方法】选取35日龄阴性健康猪30头，随机分6组，PCV2/HP-PRRSV顺序共感染组、HP-PRRSV/PCV2顺序共感染组、HP-PRRSV+PCV2同时共感染组、HP-PRRSV感染组、PCV2感染组、未接种对照组。感染后每天测量体温、观察临床症状，每周称量体重、采血。用流式细胞仪检测血液中的CD3+ CD4+ CD8-、CD3+ CD4- CD8+、γδT、NK细胞及粒细胞和单核细胞变化；免疫过氧化物酶单层细胞试验（IPMA）检测这两种病毒抗体变化；用ELISA法检测血清中IFN-γ、TNF-α、IL-10、IL-2、GM-CSF细胞因子变化；用荧光定量PCR法检测血清和组织中病毒载量。对各组试验猪进行组织病理学观察。【结果】HP-PRRSV/PCV2顺序共感染组临床症状最严重，死亡率达60%；组织和血清中病毒载量显著高于其它组，抗体滴度明显低于其它组；淋巴细胞亚群及细胞因子（尤以TNF-α）变化也最为明显。【结论】HP-PRRSV和PCV2之间存在协同致病作用，且猪群先感染HP-PRRSV后感染PCV2可明显提高猪群发病率和死亡率。本研究对临床HP-PRRSV和PCV2的综合防制提供科学依据。

关键词: 高致病性猪繁殖与呼吸综合征病毒, 猪圆环病毒2型, 共感染, 协同致病性

Abstract: 【Objective】 The purpose is to study the synergetic pathogenic effect between highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) and porcine cirovirus type 2 (PCV2), and to clarify the relation of ordinal co-infection and synergetic pathogenicity. 【Method】 Thirty 35-day-old piglets were randomly selected and separated into 6 groups which designated PCV2/HP-PRRSV ordinal co-infection group, HP-PRRSV/PCV2 ordinal co-infection group, HP-PRRSV+PCV2 simultaneous co-infection group, HP-PRRSV infection group, PCV2 infection group and control group, respectively. Clinical symptom and temperature were recorded everyday post-inoculation, meanwhile, weighing body weight and collecting blood samples. Variations of CD3+CD4+CD8-, CD3+CD4-CD8+, γδT, NK, granulocyte and mononuclear cell were determined by flow cytometry. Two kinds of antibodies against HP-PRRSV and PCV2 were quantified by immunoperoxidase monolayer assay (IPMA). Concentrations of IFN-γ, TNF-α, IL-10, IL-2, GM-CSF in serum were determined by ELISA. Pathological changes were observed through making pathological section for different organs. 【Result】 All the findings suggest that the pathogenicity of HP-PRRSV/PCV2 ordinal co-infection is conspicuously more severe than other testing groups. Mortality of this group is 60% surprisingly. Virus load in body is the highest while antibodies level is the lowest. Variation of immunocyte subgroups and cytokines (especially TNF-α) is the most conspicuous. 【Conclusion】 There is a synergetic pathogenicity of co-infection of pigs with HP-PRRSV and PCV2. If HP-PRRSV infecting precedes PCV2, the morbility and mortality will be conspicuously enhanced. This study has provided a scientific proof to control the two viruses.

Key words: highly pathogenic porcine reproductive and respiratory syndrome virus, porcine cirovirus type 2, co-infection, synergetic pathogenicity

范培虎, 危艳武, 郭龙军, 吴洪丽, 黄立平, 刘长明. 高致病性PRRSV与PCV2共感染协同致病性研究[J]. 中国农业科学, 2012, 45(18): 3859-3872.

FAN Pei-Hu, WEI Yan-Wu, GUO Long-Jun, WU Hong-Li, HUANG Li-Ping, LIU Chang-Ming. Studies on Synergetic Pathogenicity of Co-infection with Highly Pathogenic PRRSV and PCV2[J]. Scientia Agricultura Sinica, 2012, 45(18): 3859-3872.

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