稻曲病菌产孢能力及致病力测定

doi:10.3864/j.issn.0578-1752.2012.20.006

摘要/Abstract

摘要： 【目的】测定稻曲病菌（Ustilaginoidea virens）的产孢稳定性、产孢能力与致病力的相关性及在水稻品种上的致病性分化。【方法】采用液体培养和人工接种的方法对采自全国9个省（市）的150个稻曲病菌菌株进行产孢能力及田间致病力测定。【结果】3次产孢试验中产孢量标准偏差小于10.0的菌株有132个，占总数的88.0%。接种于两优培九（感病品种）的平均病情指数为67.7，其中98个菌株病情指数大于50；接种于淮稻5号（中等抗病品种）的平均病情指数为21.8；接种于武育粳3号（抗病品种）的平均病情指数为16.1，其中113个菌株病情指数小于25。稻曲病菌的产孢量与致病性呈正相关，但相关系数偏低，是中度—低度相关；根据150个稻曲病菌菌株在武育粳3号、淮稻5号和两优培九3个不同抗性品种上的致病力，将供试的150个稻曲病菌划分为7个致病类型，其中，第3致病类型（武育粳3号、淮稻5号和两优培九3个水稻品种表现为抗、抗、感）有53个菌株，占总数35.3%，为优势致病类型。【结论】稻曲病菌产孢性能较稳定，稻曲病菌的产孢量与致病力呈正相关。150个稻曲病菌菌株和3个不同抗性的水稻品种之间存在2种互作关系，大多数菌株与水稻品种抗性表现为弱互作关系，少数菌株与水稻品种抗性表现为强互作关系。

关键词: 稻曲病菌, 产孢, 致病力, 致病类型

Abstract: 【Objective】 The objective of this study is to test sporulation abilities and steady, pathogenicity, and relativity between the sporulation and pathogenicity of 150 strains of Ustilaginoidea virens. 【Method】A total of 150 strains of U. virens were collected from 9 provinces of China. Their sporulation abilities and pathogenicity were tested by liquid culture and artificial inoculation.【Result】The conidia-producing standard deviation of 132 strains was smaller than 10.0 in three conidia-producing tests, accounted for 88.0% of all strains. Three rice cultivars were inoculated with 150 strains. In the susceptible rice cultivar, Liangyoupeijiu, the average disease index of rice false smut was 67.7, the disease index of 98 strains were more than 50. In the medium resistance cultivar, Huaidao5, the average disease index was 21.8. In the resistant cultivar, Wuyujing3, the average disease index was 16.1, the disease index of 113 strains were shorter than 25. The results indicated that there was a positive relationship between the sporulation and pathogenicity, their correlation coefficient was on the low side, their correlation coefficient was medium-low. According to the pathogenicity of the 150 strains on three rice cultivars, they were divided into 7 pathogenic types. Pathogenic type III (resistant to Wuyujing 3 and Huaidao5, susceptible to Liangyoupeijiu) had 53 strains of U. virens, accounted for 35.3% of all strains, and it was a superior pathogen type. 【Conclusion】The sporulation of U. virens was steady. There was a positive relationship between the sporulation and pathogenicity. There were two interactions between 150 strains of U. virens and three rice varieties with different resistances, one was weak interaction which great majority strains showed, another was strong interaction which few strains showed.

Key words: Ustilaginoidea virens, sporulation, pathogenicity, pathogenic type

李, 燕, 于俊杰, 刘永锋, 尹小乐, 张荣胜, 俞咪娜, 陈志谊. 稻曲病菌产孢能力及致病力测定[J]. 中国农业科学, 2012, 45(20): 4166-4177.

LI YAN , YU Jun-Jie, LIU Yong-Feng, YIN Xiao-Le, ZHANG Rong-Sheng, YU Mi-Na, CHEN Zhi-Yi. Determination of Sporulation and Pathogenicity of Ustilaginoidea virens[J]. Scientia Agricultura Sinica, 2012, 45(20): 4166-4177.

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https://www.chinaagrisci.com/CN/Y2012/V45/I20/4166

参考文献

[1]Rush M C, Shahjahan A K M, Jones J P, Groth D E. Outbreak of false smut of rice in Louisiana. P1ant Disease, 2000, 84(1): 100.

[2]Yaegashi H, Fujita Y, Sonoda R. Severe outbreak of false smut of rice in 1988. Plant Protection, 1989, 43(6): 311-314.

[3]王兴国, 汤洪涛, 杨勤莲. 稻曲病粒形成过程及对健粒的影响. 植物保护, 1989, 15(5): 30-34.

Wang X G, Tang H T, Yang Q L. Grain caused by rice false smut′s forming process and the influence to the health grain. Plant Protection, 1989, 15(5): 30-34. (in Chinese)

[4]潘勋, 张淑平, 高俊全, 张晓东. 氮磷肥对稻曲病发生程度的影响. 河北农业科学, 1993(1): 18-20.

Pan X, Zhang S P, Gao J Q, Zhang X D. Influence of nitrogen and phosphorus to occurrence degree of rice false smut. Journal of Hebei Agricultural Sciences, 1993(1): 18-20. (in Chinese)

[5]Lu D H, Yang X Q, Mao J H, Ye H L, Wang Y P, Chen Y P, He Z Q, Chen F. Characterising the pathogenicity diversity of Ustilaginoidea virens in hybrid rice in China. Journal of Plant Pathology, 2009, 91(2): 443-451.

[6]董涛海, 钱钧, 徐国军, 章其江. 稻曲病对粳稻产量损失的测定. 浙江农业科学, 2003(2): 85-86.

Dong T H, Qian J, Xu G J, Zhang Q J. Rice false smut to determination of yield loss of Japonica. Journal of Zhejiang Agricultural Sciences, 2003(2): 85-86. (in Chinese)

[7]高俊, 奚本贵, 吴永芳, 吴庭友, 王德江, 赵阳. 水稻产量损失测定及经济阈值初探. 江苏农业科学, 2001(3): 36-37.

Gao J, Xi B G, Wu Y F, Wu T Y, Wang D J, Zhao Y. Measurement of the rice yield loss and first exploration of economic threshold. Journal of Jiangsu Agricultural Sciences, 2001(3): 36-37. (in Chinese)

[8]刘年喜, 王金辉, 刘二明, 李小娟, 郑和斌. 稻曲病产量损失测定及调查测报因子研究. 植物保护, 2010, 36(4): 145-147.

Liu N X, Wang J H, Liu E M, Li X J, Zheng H B. Rice yield loss caused by Ustilaginoidea virens and the factors for investigation and forecasting. Plant Protection, 2010, 36(4): 145-147. (in Chinese)

[9]季宏平. 水稻稻曲病产量损失及药剂防治的初步研究. 黑龙江农业科学, 2000(4): l8-l9.

Ji H P. Preliminary study on yield loss of rice damaged by rice false smut and chemical control to the disease. Heilongjiang Agricultural Science, 2000(4): l8-l9. (in Chinese)

[10]陈志谊, 聂亚锋, 刘永锋. 江苏省水稻品种对稻曲病的抗性鉴定及病菌致病力分化. 江苏农业学报, 2009, 25(4): 737-741.

Chen Z Y, Nie Y F, Liu Y F. Identification of rice resistant to rice false smut and the virulence differentiation of Ustilaginoidea virens in Jangsu Province. Journal of Jiangsu Agricultural Sciences, 2009, 25(4): 737-741. (in Chinese)

[11]张君成, 张炳欣, 陈志谊, 刘永锋, 陆凡. 稻曲病的接种方法及其效果初探. 中国水稻科学, 2003, 17(4): 390-392.

Zhang J C, Zhang B X, Chen Z Y, Liu Y F, Lu F. Preliminary study on inoculation method of rice false smut and its effect. Chinese Journal of Rice Science, 2003, 17(4): 390-392. (in Chinese)

[12]马慧, 刘培斌, 刘少霞, 陈丽静, 姜明兰, 钟文田. 稻曲病菌粗毒素的初步研究. 辽宁农业科学, 2001(2): 40-41.

Ma H, Liu P B, Liu S X, Chen L J, Jiang M L, Zhong W T. Preliminary study on crude toxin caused by Ustilaginoidea virens. Liaoning Agricultural Science, 2001(2): 40-41. (in Chinese)

[13]高峻. 稻曲病粒对鸡和兔的毒性. 植物保护, 1987, 13(3): 52-53.

Gao J. Toxicity of grain caused by rice false smut′s to chicken and rabbit. Plant Protection, 1987, 13(3): 52-53. (in Chinese)

[14]张君成, 陈志谊, 张炳欣, 刘永锋, 陆凡. 稻曲病的接种技术研究. 植物病理学报, 2004, 34(5): 463-467.

Zhang J C, Chen Z Y, Zhang B X, Liu Y F, Lu F. Inoculation techniques used for inducing rice false smut efficiently. Acta Phytopathologica Sinica, 2004, 34(5): 463-467. (in Chinese)

[15]唐春生, 高家樟, 黄守行, 刘二明, 刘承龙, 谢伟, 罗学艺, 肖青. 稻曲病病情分级标准的研究和应用. 湖南农业大学学报, 2000, 26(2): 122-124.

Tang C S, Gao J Z, Huang S X, Liu E M, Liu C L, Xie W, Luo X Y, Xiao Q. Research and application of classification standard of rice false smut. Journal of Hunan Agricultural University, 2000, 26(2): 122-124. (in Chinese)

[16]李松冈. 实用生物统计. 北京: 北京大学出版社, 2002: 202-207.

Li S G. Practical Biometric. Beijing: Peking University Press, 2002: 202-207. (in Chinese)

[17]杨小林, 喻大昭, 陈其志, 张舒, 吕亮. 湖北省稻瘟病生理小种的组成与分布. 植物病理学报, 2005, 35(6): 204.

Yang X L, Yu D Z, Chen Q Z, Zhang S, Lü L. Race composition and distribution of Magnaporthe grisea in Hubei Province. Acta Phytopathologica Sinica, 2005, 35(6): 204. (in Chinese)

[18]de Araujo L G, Prabhu A S, Pereira P A A . RAPD marker linked to a gene conferring resistance to race IB-9 of Pyricularia grisea in a somaclone of the rice cultivar Araguaia. Plant Cell, Tissue and Organ Culture, 2004, 78: 151-158.

[19]Consolo V F, Cordo C A, Salerno G L. DNA fingerprint and pathotype diversity of Pyricularia oryzae populations from Argentina. Australasian Plant Pathology, 2008, 37: 357-364.

[20]刘永锋, 陈志谊, 刘邮洲, 罗楚平, 聂亚锋, 于俊杰, 王晓宇, 尹小乐. 2001-2010年江苏省稻瘟病菌种群变化分析. 江苏农业学报, 2010, 26(6): 1233-1237.

Liu Y F, Chen Z Y, Liu Y Z, Luo C P, Nie Y F, Yu J J, Wang X Y, Yin X L. Changes of Magnaporthe oryzae population in Jiangsu Province from 2001 to 2010. Jiangsu Journal of Agricultural Science, 2010, 26(6): 1233-1237. (in Chinese)

[21]Le M T, Arie T, Teraoka T. Population dynamics and pathogenic races of rice blast fungus, Magnaporthe oryzae in the Mekong Delta in Vietnam. Journal of General Plant Pathology, 2010, 76: 177-182.

[22]Lee S, Wamishe Y, Jia Y, Liu G, Jia M H. Identification of two major resistance genes against race IE-1k of Magnaporthe oryzae in the indica rice cultivar Zhe733. Molecular Breeding, 2009, 24: 127-134.

[23]Adachi N, Oku T. PCR-mediated detection of Xanthomonas oryzae pv. oryzae by amplification of the 16s-23s rDNA spacer region sequence. Journal of General Plant Pathology, 2000, 66: 303-309.

[24]Tanaka K, Nakatsu S, Katsuba Z, Furuya H, Tsuchiya T, Oku T. Notes on the occurrence of pathogenic races of Xanthomonas oryzae pv.oryzae found in Hiroshima Prefecture during 2000-2003. Journal of General Plant Pathology, 2005, 71: 144-146.

[25]Oku T, Sato K, Kurao M, Matsuura S, Sakai Y, Tsuchiya T. Notes on the occurrence of pathogenic races of Xanthomonas oryzae pv. oryzae found in Hiroshima Prefecture in 1999. Journal of General Plant Pathology, 2000, 66: 332-334.

[26]Oku T, Tanaka K, Iwamoto M, Inoue Y, Ochiai H, Kaku H, Tsuge S, Tsuno K. Structural conservation of the hrp gene cluster in Xanthomons oryzae pv. oryzae. Journal of General Plant Pathology, 2004, 70: 159-167.

[27]Li Z K, Luo L J, Mei H W. A ''defeated'' rice resistance gene acts as a QTL against a virulent strain of Xanthomonas oryzae pv. oryzae. Molecular and General Genetics, 1999, 261(1): 58-63.