中国红麻炭疽病病原菌的分离鉴定及rDNA-ITS序列分析

doi:10.3864/j.issn.0578-1752.2012.17.007

中国农业科学 ›› 2012, Vol. 45 ›› Issue (17): 3515-3521.doi: 10.3864/j.issn.0578-1752.2012.17.007

中国红麻炭疽病病原菌的分离鉴定及rDNA-ITS序列分析

刘晓倩, 祁建民, 陈玉森, 陈绵才, 陈美霞, 刘伟, 方平平, 林荔辉, 陶爱芬

1.福建农林大学作物遗传育种与综合利用教育部重点实验室/农业部东南黄/红麻科学观测实验站，福州 350002
2.福建农林大学植物保护学院，福州 350002；3海南省农业科学院农业环境与植物保护研究所，海口 570100

收稿日期:2012-01-04 出版日期:2012-09-01 发布日期:2012-07-31
通讯作者: 通信作者祁建民，Tel：0591-87644898；E-mail：Qijm863@163.com。通信作者陈玉森，E-mail：13599391089@163.com
作者简介:刘晓倩，E-mail：huanyinglanghua@163.com
基金资助:
国家麻类产业技术体系建设项目（nycytx-19-512）、农业部东南黄/红麻科学观测实验站建设项目（农科教（2011）9号）、福建省南方麻类种质资源共享平台项目（2011N2002）、福建省农业五新项目（Scz10007）

Identification and rDNA-ITS Sequence Analysis of the Pathogen of Kenaf Anthracnose

LIU Xiao-Qian, QI Jian-Min, CHEN Yu-Sen, CHEN Mian-Cai, CHEN Mei-Xia, LIU Wei, FANG Ping-Ping, LIN Li-Hui, TAO Ai-Fen

1.福建农林大学作物遗传育种与综合利用教育部重点实验室/农业部东南黄/红麻科学观测实验站，福州 350002
2.福建农林大学植物保护学院，福州 350002；3海南省农业科学院农业环境与植物保护研究所，海口 570100

Received:2012-01-04 Online:2012-09-01 Published:2012-07-31

摘要/Abstract

摘要： 【目的】明确中国红麻炭疽病病原菌种类。【方法】从河南、安徽、浙江、福建4省红麻主产区采集红麻炭疽病病原菌样本，进行分离、纯化，共获得16个菌株，在致病性测定及形态学特征鉴定的基础之上，从中选取AH、HN、ZJ、FJ 4个典型病原菌菌株，对rDNA-ITS区域进行基因序列分析。【结果】AH、HN、ZJ与FJ炭疽病菌株在形态特征及致病力有明显差异；rDNA-ITS序列实际长度分别为541、545、541、535 bp，AH、HN、ZJ与FJ炭疽病菌株同源性达90%—91%，而AH、HN和ZJ 3个菌株的同源性达96%—98%。【结论】从分子水平上对中国麻区红麻炭疽病病原菌种类进行鉴定分析，证实中国红麻主产区近年炭疽病发生的病原菌类型主要有黑线炭疽菌（Colletotrichum dematium）和胶孢炭疽菌（C. gioeosporioides），其中以黑线炭疽菌致病力较强，胶孢炭疽菌致病力较弱。

关键词: 红麻, 炭疽病菌, 病原种类, 致病力, rDNA-ITS序列

Abstract: 【Objective】The objective of this study is to determine the possible pathogens of kenaf anthracnose of China.【Method】The samples of diseased kenaf were collected and isolated from Anhui, Henan, Zhejiang, Fujian Provinces of China. AH, HN, ZJ, FJ strains were selected from sixteen pathogens and their pathogenicities were determined. Identification was carried out by using traditional morphological methods and sequencing of rDNA-ITS. 【Result】 The differences among AH, HN, ZJ and FJ strains in morphological characteristics and pathogenicities were relatively abundant. The rDNA-ITS length of AH, HN, ZJ, and FJ strains was 541, 545, 541, and 535 bp, respectively. The homology between FJ strain and other strains was 90%-91%, and the homology among AH, HN, ZJ strains was 96%-98%.【Conclusion】The pathogens of kenaf anthracnose were identified by analysis at the molecular level. It is confirmed that kenaf anthracnose in China is caused by C. dematium and C. gioeosporioides, and the pathogenicity of C. dematium is greater than C. gioeosporioides.

Key words: kenaf, Colletotrichum, pathogen species, pathogenicity, rDNA-ITS sequence

刘晓倩, 祁建民, 陈玉森, 陈绵才, 陈美霞, 刘伟, 方平平, 林荔辉, 陶爱芬. 中国红麻炭疽病病原菌的分离鉴定及rDNA-ITS序列分析[J]. 中国农业科学, 2012, 45(17): 3515-3521.

LIU Xiao-Qian, QI Jian-Min, CHEN Yu-Sen, CHEN Mian-Cai, CHEN Mei-Xia, LIU Wei, FANG Ping-Ping, LIN Li-Hui, TAO Ai-Fen. Identification and rDNA-ITS Sequence Analysis of the Pathogen of Kenaf Anthracnose[J]. Scientia Agricultura Sinica, 2012, 45(17): 3515-3521.

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中国红麻炭疽病病原菌的分离鉴定及rDNA-ITS序列分析

Identification and rDNA-ITS Sequence Analysis of the Pathogen of Kenaf Anthracnose

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