亚慢性镉暴露对五指山猪肝脏生化指标的影响

doi:10.3864/j.issn.0578-1752.2012.03.019

摘要/Abstract

摘要： 【目的】研究亚慢性镉（Cd）暴露对五指山猪（Wuzhishan Pig, WZSP）肝脏生化指标的影响，寻找亚慢性镉暴露致肝脏损伤的敏感性生化指标，为镉肝脏毒性的监测及预防提供科学依据。【方法】将3月龄健康WZSP 20头随机分为5组，分别饲喂添加镉0.0 mg•kg-1(Ⅰ组)、0.5 mg•kg-1（Ⅱ组）、2.0 mg•kg-1（Ⅲ组）、8.0 mg•kg-1（Ⅳ组）及32.0 mg•kg-1（Ⅴ组）的全价饲料，持续喂养100 d。试验期，每20 d测1次饲料镉含量，血清天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）和γ-谷氨酰胺基转移酶（γ-GGT）活性；试验结束后测定血液和肝脏中镉的含量。【结果】随着镉暴露剂量的增加，与Ⅰ组相比，各剂量组肝脏镉含量(L-Cd)显著升高（P＜0.05），Ⅳ组、Ⅴ组血液镉含量(B-Cd)显著升高（P＜0.05）；与Ⅰ组相比，Ⅱ组和Ⅲ组在镉暴露80 d后ALT活性显著升高（P＜0.05），Ⅳ组在镉暴露60 d后显著升高（P＜0.05），Ⅴ组在镉暴露20 d后显著升高（P＜0.05）；与Ⅰ组相比，Ⅱ组和Ⅲ组在镉暴露20 d后AST活性显著升高（P＜0.05），40 d后差异不显著（P＞0.05），Ⅳ组和Ⅴ组在镉暴露100 d后显著升高（P＜0.05）；与Ⅰ组相比，Ⅱ组、Ⅳ组和Ⅴ组在镉暴露100 d后GGT活性显著升高（P＜0.05）；相关分析结果显示，镉暴露量、B-Cd和L-Cd三者间极显著相关（P＜0.01），ALT活性与镉暴露量和L-Cd显著相关（P＜0.05）。【结论】高于0.5 mg•kg-1的亚慢性镉暴露可造成肝脏损伤，导致ALT、AST、GGT活性升高。

关键词: 五指山猪, 镉, 肝脏, 亚慢性毒性

Abstract: 【Objective】 The objective of this study was to look for a sensitive monitoring index of liver injury induced by cadmium and provide a scientific basis for cadmium monitoring and prevention by studying the influences of subchronic cadmium exposure on liver biochemical indexes.【Method】Twenty healthy 3-month-old Wuzhishan Pig (WZSP )were divided randomly into 5 groups, and then fed with basal diet containing 0.0 mg•kg-1 (groupⅠ), 0.5 mg•kg-1 (group Ⅱ), 2.0 mg•kg-1 (group Ⅲ), 8.0 mg•kg-1 (group Ⅳ), 32.0 mg•kg-1 (group Ⅴ) doses of cadmium respectively over one hundred days. Blood were collected every 20 days to determine the activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and γ-glutamyltransferase (γ-GGT). Blood cadmium and liver cadmium were determined at the end of experiment. 【Result】 Compared with group Ⅰ, ALT activity in groups Ⅱ and Ⅲ increased significantly after exposing to cadmium for 80 days (P＜0.05), followed by 60 days for group Ⅳ and 20 days for group Ⅴ but not within 40 days. AST activities in groups Ⅱ and Ⅲ increased significantly after exposing to cadmium for 20 days (P＜0.05) but not within 40 days (P＞0.05), while groups Ⅳ and Ⅴ increased significantly within 100 days (P＜0.05). GGT activities in groups Ⅱ, Ⅳ, and Ⅴ all increased significantly after exposing to cadmium for 100 days (P＜0.05). Liver cadmium and blood cadmium in groups Ⅳ and Ⅴ increased significantly compared to group Ⅰ(P＜0.05). There was a significant correlation among cadmium exposure, blood cadmium and liver cadmium at the 0.01 level, ALT activity was significantly correlated with cadmium exposure and liver cadmium at the 0.05 level. 【Conclusion】 Liver injury can be induced by subchronic cadmium exposure at concentrations higher than 0.5 mg•kg-1. Serum ALT activity and blood cadmium can be used respectively as a liver effective biomarker and exposure biomarker of liver injury induced by subchronic cadmium exposure.

Key words: Wuzhishan Pig, cadmium, liver, subchronic toxicity

吴小胜, 魏帅, 魏益民, 郭波莉, 杨鸣琦, 刘晓玲. 亚慢性镉暴露对五指山猪肝脏生化指标的影响[J]. 中国农业科学, 2012, 45(3): 562-568.

WU Xiao-Sheng, WEI Shuai, WEI Yi-Min, GUO Bo-Li, YANG Ming-Qi, LIU Xiao-Ling. Effect of Sub-chronic Cadmium Exposure on Liver Biochemical Index in Wuzhishan Pig[J]. Scientia Agricultura Sinica, 2012, 45(3): 562-568.

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