中华蜜蜂csd多态性分析

doi:10.3864/j.issn.0578-1752.2011.23.018

摘要/Abstract

摘要： 【目的】分析不同地理群体中华蜜蜂（Apis cerana cerana）csd的多态性。【方法】以吉林长白山、海南海口、广西南宁、湖北神农架和江西靖安5个省市的中蜂工蜂为试验材料，提取每个工蜂样品的基因组DNA，对csd 3区进行PCR扩增、克隆和测序，分析csd的多态性。【结果】对中国5个省市的中蜂csd 3区基因组序列进行了克隆测序，获得32条csd基因单倍型，其中吉林长白山和江西靖安群体csd的多态性显著高于其它群体，而吉林长白山群体和江西靖安群体之间，广西南宁群体、海南海口群体和湖北神农架群体之间csd的多态性没有显著差异。利用csd进行群体分析表明江西靖安中蜂与吉林长白山中蜂的核苷酸分歧度和遗传距离均最大，湖北神农架中蜂与广西南宁中蜂之间核苷酸分歧度和遗传距离最小。系统进化树表明来自5个地理群体的单倍型在进化树上混杂在一起，并没有按照地理来源分为5支。【结论】中华蜜蜂csd在各个地理群体中均具有很高的多态性，并且多态性水平在群体间有差异。

关键词: 中华蜜蜂, csd, 多态性, 单倍型

Abstract: 【Objective】 The objective of this study is to analyze the polymorphism of csd in different geographical populations of Chinese honeybee (Apis cerana cerana) (Hymenoptera: Apidae). 【Method】 Worker bees collected from Changbaishan (Jilin), Haikou (Hainan), Nanning (Guangxi), Shennongjia (Hubei) and Jing’an (Jiangxi) were used as materials, and genome DNA was extracted from each sample for PCR amplification of the csd region 3, PCR products were cloned and sequenced, polymorphism of csd in different geographical populations were analyzed by using the obtained sequences. 【Result】 The genomic region 3 of csd in Apis cerana cerana from 5 geographical populations were cloned and 32 csd haplotypes were obtained. The polymorphism of csd in Changbaishan (Jilin) and Jing’an (Jiangxi) populations were significantly higher than those in Nanning, Haikou and Shennongjia populations, while the polymorphism of csd between Changbaishan (Jilin) and Jing’an (Jiangxi) populations, and that between Nanning (Guangxi), Haikou (Hainan) and Shennongjia (Hubei) populations showed no significant difference. Meanwhile, population analysis based on csd haplotypes showed that the nucleotide divergence and genetic distance between Changbaishan (Jilin) and Jing’an (Jiangxi) populations were maximal, while those between Shennongjia (Hubei) and Nanning (Guangxi) populations were minimal. The phylogenetic analysis showed that all the haplotypes from these five geographical populations were mixed on the phylogenetic tree, not from 5 clade according to different geographical origins. 【Conclusion】 csd has a high level of polymorphism in all these populations, moreover, the polymorphism level showed some difference among populations.

Key words: Apis cerana cerana, csd, polymorphism, haplotype

刘志勇, 王子龙, 王欢, 曾志将. 中华蜜蜂csd多态性分析[J]. 中国农业科学, 2011, 44(23): 4911-4917.

LIU Zhi-Yong, WANG Zi-Long, WANG Huan, ZENG Zhi-Jiang. Polymorphism Analysis of csd in Five Populations of Chinese Honeybee[J]. Scientia Agricultura Sinica, 2011, 44(23): 4911-4917.

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https://www.chinaagrisci.com/CN/Y2011/V44/I23/4911

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