苦瓜皂苷和多糖的连续提取工艺及其对α-葡萄糖苷酶的抑制作用

doi:10.3864/j.issn.0578-1752.2011.19.016

摘要/Abstract

摘要： 【目的】建立超声-微波法连续提取苦瓜皂苷和多糖的优化工艺，并评价其对α-葡萄糖苷酶的抑制作用。【方法】采用二次回归正交旋转组合设计优化超声-微波法连续提取苦瓜中皂苷和多糖的工艺条件，采用4-硝基酚-2-D吡喃葡萄糖苷(PNPG)法测定皂苷和多糖提取物对α-葡萄糖苷酶抑制率。【结果】以含水质量分数13%的苦瓜干为原料，先提取皂苷后提取多糖的条件为，将其粉碎至200目后，以体积分数75%乙醇为溶剂，料液比 1﹕18，超声-微波协同萃取（50 W，20 kHz）16 min，在该条件下，皂苷提取率达2.51%。提取皂苷后的残渣，继续以水为溶剂，料液比1﹕14，pH 8.0，时间23 min，温度84℃，超声-微波协同萃取（50 W，20 kHz）20 min，多糖提取率达12.86%。苦瓜皂苷和多糖提取物对α-葡萄糖苷酶均有不同程度的抑制作用，其IC50 分别为1.03 mg•mL-1和10.73 mg•mL-1，苦瓜皂苷的抑制效果显著强于多糖的（P＜0.05）。【结论】采用超声-微波协同作用连续提取苦瓜皂苷和多糖工艺，可有效利用原料、提高产出和效率，所得苦瓜皂苷和多糖提取物对α-糖苷酶均表现抑制作用，显示一定的降糖潜力。

关键词:

苦瓜, 皂苷, 多糖, 连续提取, &alpha, -葡萄糖苷酶

Abstract: 【Objective】 The technical process and suitable conditions for continuous extraction of saponin and polysaccharide from Momordica charantia L. by ultrasonic-microwave synergistic extraction were established and to evaluate their inhibition effects on α-glucosidase 【Method】 Quadratic Regression Rotational Combinational Design was applied to optimize the extraction process of saponin and polysaccharide from Momordica charantia L. using continuous ultrasonic-microwave synergistic extraction method. The method with substrate 4-nitrophenol-2-?-D-glucopyranoside(PNPG) was used to measure the α-glucosidase inhibitory effect of saponin and polysaccharide extract. 【Result】 The optimum conditions for saponin and polysaccharide extraction form Momordica charantia L. were as follows: the dried Momordica charantia L. with 13% of water content was grinded to pass a 200 μm sieve; the solvent was 75% enthanol and the ratio of material to solvent was 1:18; ultrasonic-microwave synergistic extraction for 16 min (the power of microwave was 50 W and the ultrasonic frequency was 20 kHz). Under this condition the extraction yield of saponin from Momordica charantia L. was 2.51%. The residue was further dissolved in water of pH 8.0 for extraction of polysaccharide, the ratio of material to solvent was 1:14; the ultrasonic-microwave synergetic extraction time was 23 min (the power of microwave was 50 W and the ultrasonic frequency was 20 kHz) at a temperature of 84℃. Under these conditions, the extraction yield of polysaccharide from Momordica charantia L. was 12.86%. Both of the extracts of saponin and polysaccharide from Momordica charantia L. showed an obvious inhibitory activity against α-glucosidase with values of 1.03 and 10.73 mg•mL-1, respectively.【Conclusion】 The raw materials were utilized effectively and the extraction yield and efficiency were increased using a method of ultrasonic-microwave synergistic extraction. The extracted saponin and polysacchride from Momordica charantia L. showed an obvious inhibition effect on α-glucosidase with promising of blood sugar reducing potentials.

Key words:

Momordica charantia L., saponin, polysaccharide, continuous extraction, α-glucosidase

王琪, 邓媛元, 张名位, 张瑞芬, 张雁, 唐小俊, 魏振承, 池建伟. 苦瓜皂苷和多糖的连续提取工艺及其对α-葡萄糖苷酶的抑制作用[J]. 中国农业科学, 2011, 44(19): 4058-4065.

WANG Qi, DENG Yuan-Yuan, ZHANG Ming-Wei, ZHANG Rui-Fen, ZHANG Yan, TANG Xiao-Jun, WEI Zhen-Cheng, CHI Jian-Wei. Continuous Extraction of Saponin and Polysaccharide from Momordica charantia L. and Their Inhibitory Effect on α-glucosidase[J]. Scientia Agricultura Sinica, 2011, 44(19): 4058-4065.

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https://www.chinaagrisci.com/CN/Y2011/V44/I19/4058

参考文献

[1]Liva H, Michi T, Shigeru T, Munehiro O, Orie M, Masayuki U, Yoshinori A. Momordica charantia constituents and antidiabetic screening of the isolated major compounds. Chemical and Pharmaceutical Bulletin, 2006, 54(7): 1017-1021.

[2]Nivitabishekam S N, Asad M, Prasad V S. Pharmacodynamic interaction of Momordica charantia with rosiglitazone in rats. Chemico-Biological Interactions, 2009, 177: 247-253.

[3]王先远, 金宏, 徐志勤, 高兰兴. 苦瓜皂甙降血糖作用及其机制初探. 氨基酸和生物资源, 2001, 23(3): 42-45.

Wang X Y, Jin H, Xu Z Q, Gao L X. Study on hypoglycemic effect of momordica saponins and its mechanism. Amino Acids and Biotic Resources, 2001, 23(3): 42-45. (in Chinese)

[4]徐斌, 董英, 张慧慧, 崔恒林, 陆琪. 苦瓜多糖对链脲佐菌素诱导糖尿病小鼠的降血糖效果. 营养学报, 2006, 28(5): 401-408.

Xu B, Dong Y, Zhang H H, Cui H L, Lu Q. The antihyperglycemic effects of polysaccharide from Momordica charantia in STZ-induced diabetic mice. Acta Nutrimenta Sinica, 2006, 28(5): 401-408. (in Chinese)

[5]Parichat B, Artiwan S. Extraction of phenolic compounds from fruits of Bitter Melon (Momordica charantia) with subcritical water extraction and antioxidant activities of these extracts. Chiang Mai Journal Science, 2008, 35(1): 123-130.

[6]田力东, 张名位, 郭祀远, 张雁, 张瑞芬. 苦瓜皂苷的提取工艺优化研究. 现代食品科技, 2007, 23(1): 40-44.

Tian L D, Zhang M W, Guo S Y, Zhang Y, Zhang R F. Study on optimization of extraction technology of saponin from Momordica charantia L. Modern Food Science Technology, 2007, 23(1): 40-44. (in Chinese)

[7]陈鋆, 刘振香, 吴昌胜. 苦瓜中提取苦瓜皂苷的工艺研究. 广州化工, 2010, 38(1): 59-61.

Chen Y. Liu Z X. Wu C S. Study on extracting technology of saponins from bitter melon. Guangzhou Chemical Industry, 2010, 38(1): 59-61. (in Chinese)

[8]钟平, 张小联. 微波辐射诱导萃取法从苦瓜中提取苦瓜甙. 材料导报, 2007, 21(11A): 22-23.

Zhong P, Zhang X L. Extraction of glycoside from balsam pear by microwave radiation. Materials Review, 2007, 21(11A): 22-23. (in Chinese)

[9]陈志强, 李贞景, 王昌禄. 响应面法优化超声提取苦瓜皂苷工艺条件的研究. 氨基酸和生物资源, 2007, 29(4): 21-25.

Chen Z Q, Li Z J, Wang C L. Study on optimum technique of extracting total saponins from bitter melon by supersonic wave. Amino Acids and Biotic Resources, 2007, 29(4): 21-25. (in Chinese)

[10]黄婧, 张名位, 辛修锋, 张雁, 张瑞芬. 苦瓜粗多糖提取工艺的优化. 农业机械学报, 2007, 38(6): 112-116.

Huang J, Zhang M W, Xing X F, Zhang Y, Zhang R F. Optimization of extraction parameters for Momordica charantia polysaccharides, Transactions of the Chinese Society for Agricultural Machinery, 2007, 38(6): 112-116. (in Chinese)

[11]吴茄笛, 阚国仕, 陈红漫, 李贺民. 双酶水解法提取水溶性苦瓜多糖的研究. 安徽农业科学, 2005, 33(1): 73-74.

Wu J D, Gan G S, Chen H M, Li H M. Studies on the Enzyme Hydrolysis of Soluble Polysaccharide from Momordica charantia. Journal of Anhui Agricultral Sciences, 2005, 33(1): 73-74. (in Chinese)

[12]李宏睿, 范琳琳, 徐明生, 黄占旺, 张文波. 苦瓜多糖超声波辅助提取工艺优化. 食品与机械, 2010, 26(2): 107-109.

Li H R, Fan L L, Xu M S, Huang Z W, Zhang W B. Optimization on extraction technology of Momordica charantia polysaccharides by ultrasound. Food & Machinery, 2010, 26(2): 107-109. (in Chinese)

[13]全吉淑, 尹学哲, 田中真实, 金泽武道. 大豆胚轴提取物的降糖作用及其机制研究. 营养学报, 2004, 26(3): 207-210.

Quan J S, Yin X Z, Tanaka M, Kanazawa T. The hypoglycemic effects of soybean hypocotyls extract in diabetic rats and their mechanism. Acta Nutrimenta Sinica, 2004, 26(3): 207-210. (in Chinese)

[14]田力东, 张名位, 郭祀远, 张瑞芬, 池建伟, 魏振承, 张雁, 唐小俊. 不同苦瓜品种的皂苷含量及对α-葡萄糖苷酶活性抑制作用的比较. 中国农业科学, 2008, 41(10): 3415-3421.

Tian L D, Zhang M W, Guo S Y, Zhang R F, Chi J W, Wei Z C, Zhang Y, Tang X J. Comparison of saponin contents of different varieties of Momordica charantia L. and their inhibition on α-glucosidase activity. Scientia Agricultura Sinica, 2008, 41(10): 3415-3421. (in Chinese)

[15]田丽梅, 王旻, 陈卫. 枸杞多糖对α-葡萄糖苷酶的抑制作用. 华西药学杂志, 2006, 21(2): 131-133.

Tian L M, Wang M, Chen W. Inhibition effects of Lyceum barbarum polysaccharides on α-glucosidase. West China Journal of Pharmaceutical Sciences, 2006, 21(2): 131-133. (in Chinese)

[16]Tiabou T A, Nahar K S, Fuendjiep V, Ngandeu F, Ngono N A, Choudhary M I. α-Glucosidase inhibitors from Millettia conraui. Chemical and Pharmaceutical Bulletin, 2007, 55(9): 1402-1403.

[17]Sin H N, Yusof S. Optimization of hot water extraction for sapodilla juice using response surface methodology. Journal of Food Engineering, 2006, 74(3): 352-358.

[18]Valachovic P, Pechova A, Mason T J. Towards the industrial production of medicinal tincture by ultrasound assisted extraction. Ultrasonics Sonochemistry, 2001, 8: 111-117.

[19]Hromadkova Z, Ebringerova A, Valachovi P. Comparison of classical and ultrasound-assisted extraction of polysaccharides from Salvia officinalis L. Ultrasonics Sonochemistry, 1999, 5: 163-168.

[20]Hromadkova Z, Ebringerova A, Valachovi P. Ultrasound-assisted extraction of water-soluble polysaccharides from the roots of valerian. Ultrasonics Sonochemistry, 2002, 9: 37-44.

[21]李健, 任惠峰, 陈姝娟, 刘宁, 雄文波. 微波辅助萃取苦瓜总皂苷工艺研究. 中国食品学报, 2009, 9(3): 78-83.

Li J, Ren H F, Chen S J, Liu N, Xiong W B. Studies on extracting of total saponins in bitter melon by microwave-assisted extractive method. Journal of Chinese Institute of Food Science and Technology, 2009, 9(3): 78-83. (in Chinese)

[22]Donatella M C, Ronaldo P F. α-Glucosidase inhibitors prevent diet-induced increases in intestinal sugar transport in diabetic mice. Metabolism Clinical and Experimental, 2006, 55: 832-841.

[23]陈海敏, 严小军, 林伟. α-葡萄糖苷酶抑制剂的构效关系. 中国生物化学与分子生物学报, 2003, 19(6): 780-784.

Chen H M, Yan X J, Lin W. Relationship between structure and function of α glucosidase inhibitiors. Chinese Journal of Biochemistry and Molecular Biology, 2003, 19(6): 780-784. (in Chinese)

[24]Sook Y L, Seok H E, Yong K K, Nam I P, Sang U P. Cucurbitane-type triterpenoids in Momordica charantia Linn. Journal of Medicinal Plants Research, 2009, 3(13):1264-1269.

[25]程霜, 崔庆新, 王勇. 水溶性苦瓜多糖的提取与测定. 郑州粮食学院学报, 2000, 21(2): 53-59.

Cheng S, Cui Q X, Wang Y. Extraction and determination of water-soluble polysaccharide of Momordica charantia. Journal of Zhengzhou Grain College, 2000, 21(2): 53-59. (in Chinese)

[26]陈静, 程永强, 刘晓庆, 孙健, 李里特. 食品中α-葡萄糖苷酶抑制剂的研究进展. 食品科学, 2007, 28(4): 360-363.

Chen J, Cheng Y Q, Liu X Q, Sun J, Li L T. Research progress review on α-glucosidase inhibitor in food. Food Science, 2007, 28(4): 360-363. (in Chinese)