荧光辅助糖电泳检测葡甘露寡糖条件的分析

doi:10.3864/j.issn.0578-1752.2011.15.016

中国农业科学 ›› 2011, Vol. 44 ›› Issue (15): 3206-3213.doi: 10.3864/j.issn.0578-1752.2011.15.016

荧光辅助糖电泳检测葡甘露寡糖条件的分析

姚喜梅, 石波, 刘程程, 梁平, 李静梅

1. 中国农业科学院饲料研究所
2. 中国农业科学院作物科学研究所

收稿日期:2011-03-16 修回日期:2011-05-03 出版日期:2011-08-01 发布日期:2011-05-05
通讯作者: 通信作者石波，Tel：010-82106075；E-mail：shibo@mail.caas.net.cn
基金资助:
国家“863”计划项目（2006AA10Z337和2007AA091601-2）

Analysis of Glucomanno-Oligosaccharides by Fluorophore-Assisted Carbohydrate Electrophoresis

YAO Xi-Mei, SHI Bo, LIU Cheng-Cheng, LIANG Ping, LI Jing-Mei

1. 中国农业科学院饲料研究所
2. 中国农业科学院作物科学研究所

Received:2011-03-16 Revised:2011-05-03 Online:2011-08-01 Published:2011-05-05
Contact: Xi-Mei YAO

摘要/Abstract

摘要： 【目的】探索荧光辅助糖电泳（FACE）检测葡甘露寡糖组分的条件。【方法】以魔芋葡甘露寡糖为原料，对比3种荧光衍生剂的电泳分离效果，在此基础上研究分离胶浓度、衍生化反应条件及样品杂质对FACE分离效果的影响，确定FACE分析葡甘露寡糖的最佳条件。【结果】FACE分析葡甘露寡糖最佳条件是：荧光衍生剂为ANTS，分离胶浓度25%，ANTS用量 2 μL，衍生化反应温度37℃，衍生化反应时间16 h。【结论】FACE对葡甘露寡糖组分有很好的分离效果，使用本方法可以确定葡甘露寡糖中至少含有14种组分，为葡甘露寡糖的定性定量分析提供一种有效的检测手段。

关键词: 葡甘露寡糖, 荧光辅助糖电泳, ANTS, 检测

Abstract: 【Objective】 The objective of this study was to explore the conditions of FACE detecting glucomanno- oligosaccharides. 【Method】 Konjac glucomanno-oligosaccharides was used as the substrate, the optimal parameters were determined by investigating the effects of 3 kinds of fluorescer, the concentration of resolving gel, the conditions of derivatization reaction and sample impurities on FACE. 【Result】 The optimal conditions of FACE used in detecting glucomanno-oligosaccharides were as follows: fluorescer ANTS, concentration of resolving gel 25%, content of ANTS 2 μL, derivation reaction temperature 37℃ and derivation reaction time 16 h.【Conclusion】These findings show that FACE has a high resolution to glucomanno- oligosaccharides, and 14 kinds of components were detected by FACE, which provides an effective method for qualitative and quantitative analysis of glucomanno-oligosaccharides.

Key words: Glucomanno-oligosaccharides, FACE, ANTS, Detecting

姚喜梅, 石波, 刘程程, 梁平, 李静梅. 荧光辅助糖电泳检测葡甘露寡糖条件的分析[J]. 中国农业科学, 2011, 44(15): 3206-3213.

YAO Xi-Mei, SHI Bo, LIU Cheng-Cheng, LIANG Ping, LI Jing-Mei. Analysis of Glucomanno-Oligosaccharides by Fluorophore-Assisted Carbohydrate Electrophoresis[J]. Scientia Agricultura Sinica, 2011, 44(15): 3206-3213.

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荧光辅助糖电泳检测葡甘露寡糖条件的分析

Analysis of Glucomanno-Oligosaccharides by Fluorophore-Assisted Carbohydrate Electrophoresis

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