关键词: 猪活体性状, 数量性状基因座, 微卫星标记, 区间定位, 资源群

Abstract:

【Objective】Pork is an important food source now representing forty-three percent of red meat consumed in the world and is a valuable resource economically in China. It is vital to identfy QTLs associated with economically important traits in pig. 【Method】Sixteen Chinese indigenous Lantang sows were crossed with eight Landrace pig boars to construct the resource population. According to the pig linkage map of USDA-MARC2.0 and the distance between adjacent markers seleted about 10-20 cM intervals, twenty-one microsatellite DNA markers on pig chromosomes 1, 4 and 8 were used for genotyping of the parents F1 and F2 with ABI 377 and then were analysed to map QTL in the web sit (http://latte.cap.ed.ac.uk) with QTL Express software package. The gene frequence was calculated. 【Result】Two putative QTL containing a QTL for body height (BodyHh) and another QTL for body length (BodyLh) found at 68 cM on SSC1 and at 72cM on SSC4 both reached at least suggestive significance at the chromosome-wise (P＜0.05), respectively. The former closely linked to SW2185 (67.6cM), explaining 2.22% of phenotypic variation and the latter was located in between SW839 and SW0214.【Conclusion】Two putative QTL including a body height QTL on SSC1 and a body length on SSC4 were identified. These results will facilitate fine mapping efforts to identify genes controlling live traits that can be incorporated into marker-assisted selection programs to accelerate genetic improvement in pig populations.

Key words: pig live trait, quantitative trait loci, microsatellite marker, interval mapping, resource population