ramie (Boehmeria nivea),inbred line clones,ISSR,genetic relationship,"/> <font face="Verdana">苎麻自交无性繁殖系遗传关系的ISSR分析</font>

中国农业科学 ›› 2011, Vol. 44 ›› Issue (8): 1543-1552 .doi: 10.3864/j.issn.0578-1752.2011.08.002

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

苎麻自交无性繁殖系遗传关系的ISSR分析

刘立军,王绪霞,汪波,彭定祥   

  1. 华中农业大学植物科学技术学院
  • 收稿日期:2010-06-23 修回日期:2010-08-08 出版日期:2011-04-15 发布日期:2011-04-15
  • 通讯作者: 彭定祥

Analysis of Genetic Relationship of Ramie (Boehmeria nivea) Inbred Line Clones as Revealed by ISSR

LIU Li-jun, WANG Xu-xia, WANG Bo, PENG Ding-xiang   

  1. 华中农业大学植物科学技术学院
  • Received:2010-06-23 Revised:2010-08-08 Online:2011-04-15 Published:2011-04-15

摘要:

【目的】通过ISSR分子标记研究苎麻自交无性繁殖系资源之间的遗传关系,为育种及科学合理保存和利用现有种质资源提供理论依据和技术支持。【方法】利用ISSR分子标记对40份自交无性繁殖系(8份来自巴西,32份来自中国5个主产省)进行遗传关系分析。【结果】30条ISSR引物对40份材料的DNA模板进行扩增和电泳检测,扩增总条带数为116条,多态性达78.45%,Nei's平均遗传距离为0.2405,平均Shannon指数为0.3679,每条引物扩增2—10条条带。根据材料的地理位置来源,将40份自交无性繁殖系分为6个类群,多态位点百分率(pp)在23.28%—67.24%。Nei's遗传距离在0.0964—0.2285,Shannon指数在0.1408—0.3310。第一、第二、第三主成分的贡献率分别为72.67%、5.36%和2.11%,累计贡献率为80.15%。【结论】主成分分析与采用ISSR标记进行聚类的结果十分一致,利用ISSR标记技术可较准确地分析苎麻自交无性繁殖系的遗传多样性及亲缘关系。

关键词: 苎麻, 自交无性繁殖系, ISSR, 遗传关系

Abstract:

【Objective】ISSR markers were used to study the genetic relationship and investigate the genetic diversity and genetic structure for providing a theoretical basis and technical support for appropriate conservation and application of existing genetic resources of ramie inbred line clones. 【Method】Genetic relationship of 40 inbred line clones (including 8 ramie inbred line clones from Brazil and 32 from 5 provinces of China)were analyzed using ISSR markers. 【Result】The genomic DNAs of 40 inbred line clones were amplified by PCR with 30 polymorphic ISSR primers, which produced 116 bands from 200 bp to 2 000 bp bands were polymorphism. While the polymorphism of ISSR was 78.45% (91 bands out of 116), ranging from 2 (ISSR6) and 10 (ISSR22), with an average of 3.87. Pairwise comparison was made among all the inbred line clones included in this study. The Nei's genetic distance was 0.2405, Shannon's Information index was 0.3679. Based on the appearance of the markers, the genetic relationships were analyzed using UPGMA and the genetic Dice coefficients were calculated. According to the regions of origin of 40 ramie inbred line clones, they were classified into 6 groups. The Nei's genetic distance was between 0.0964 and 0.2285, Shannon's Information index was from 0.1408 to 0.3310. The Mantel examination between cluster analysis and coefficients matrix showed good fitness. The results of principal components analysis showed that the accumulative variance contribution of the first three principal components was 80.15%, the variance contribution of the first principal component was 72.67%, and the second principal and third principal components were 5.63% and 2.11%, respecitively. 【Conclusion】The results of hierarchical cluster analysis were commendably in accordance with principal components analysis. The genetic relationship and genetic diversity of ramie inbred line clones could be assessed efficiently by ISSR markers.

Key words: ramie (Boehmeria nivea)')">ramie (Boehmeria nivea), inbred line clones, ISSR, genetic relationship