关键词: 石榴皮多酚, 抗氧化活性, HPLC

Abstract:

【Objective】 The purpose of the experiment is to study the composition of pomegranate peel polyphenols (PPPs) and its antioxidant activities in vitro and in vivo. 【Method】 The components and the contents of PPPs were analysed by HPLC, and the antioxidant activities in vitro of PPPs were measured by the methods of deoxidization capability, superoxide anion radical scavenging capacity, hydroxyl radical scavenging capacity, DPPH radical scavenging capacity and anti-lipid peroxidation capacity. The effects of PPPs on antioxidant indexes of blood, liver and brain tissue in vivo were investigated by using mice as the experimental materials. 【Result】 Nine phenolic compositions were identified from PPPs extracts, in which the pulicalagin is the main component with the content of 65.75% of total phenols. Antioxidant activities in vitro tests showed that PPPs had high deoxidization capacity, scavenging free radicals activities and anti-lipid peroxidation capacity. Animal experiments showed that PPPs could raise the SOD activities in blood, liver and brain tissue, as well as CAT, GSH-Px activities in blood and liver, but reduce the contents of MDA in blood, liver and brain tissue. 【Conclusion】Pomegranate peel are rich in polyphenols with the main component of pulicalagin, and it has significant antioxidant activities. It is concluded that PPPs may be exploited as a good natural antioxidant as well as an important base material of functional foods and biomedicinals.

Key words: pomegranate peel polyphenols, antioxidation activities, HPLC