中国农业科学 ›› 2009, Vol. 42 ›› Issue (8): 2949-2957 .doi: 10.3864/j.issn.0578-1752.2009.08.038

• 兽医 • 上一篇    下一篇

2007年中国部分地区猪圆环病毒2型分子流行病学分析

张建武,庄金山,刘长龙,王小敏,袁世山

  

  1. (中国农业科学院上海兽医研究所/中国动物卫生与流行病学中心上海分中心,动物传染病防治研究室/农业部动物寄生虫学重点开放实验室)
  • 收稿日期:2008-07-26 修回日期:2009-02-12 出版日期:2009-08-10 发布日期:2009-08-10
  • 通讯作者: 袁世山

Molecular Epidemiology Analysis on Porcine Circovirus Type 2 in Some Regions of China in 2007

ZHANG Jian-wu, ZHUANG Jin-shan, LIU Chang-long, WANG Xiao-min, YUAN Shi-shan
  

  1. (中国农业科学院上海兽医研究所/中国动物卫生与流行病学中心上海分中心,动物传染病防治研究室/农业部动物寄生虫学重点开放实验室)
  • Received:2008-07-26 Revised:2009-02-12 Online:2009-08-10 Published:2009-08-10
  • Contact: YUAN Shi-shan

摘要:

【目的】对采集自上海、江西、山东、江苏、河南和湖南等省的257份进行了PCV2的检测,确定中国PCV2感染情况及分子特征。【目的】设计一对针对PCV2 ORF2的引物,利用PCR对其进行PCV2基因组的检测,对PCV2阳性的样品,进行了ORF2的克隆、序列测定和分析。【结果】结果显示PCV2阳性率为24.51%(63/257),并能从健康猪群中检测的阳性;各PCV2分离株间ORF2核苷酸序列同源性为84.4%~100%,氨基酸同源性为82.5%~100%,与所属群的不同而不同。【结论】中国有2个群的PCV2毒株在流行,以PCV2b群为主(70.97%,22/31),但也有PCV2a群毒株(29.03%,9/31)的流行,同时PCV2流行的基因型没有明显的地域间差异。部分毒株符合高致病性PCV2的特征,暗示中国已存在高致病性PCV2的流行。

关键词: 猪圆环病毒2型, 开放阅读框架2, 基因型

Abstract:

【Objective】 A pair of primer was designed for detection of porcine circovirus type 2 from Shanghai, Jiangxi, Shandong, Jiangsu, Henan and Hunan in 2007. 【Result】 Results indicated that the positive percentage of PCV2 was 24.51% (63/257) and positive samples were detected from healthy pigs. Gene amplificated from PCR product was cloned and sequenced. Sequence analysis illustrated that nucleotide and amino acid homology of ORF2 of PCV2 isolates were 84.4%-100% and 82.5%-100%, respectively, and defferent belong to defferent groups . 【Conclusion】 There were two groups of PCV2 strains prevailing in China, the PCV2b group strains were predominant prevailing strain (70.97%, 22/31) , but the PCV2a group strains were prevailing also. There was no difference in genotype of PCV2 isolated from defferent geographical regions. Some PCV2 strains owned character of high pathogenic PCV2 implied that there was a high pathogenic PCV2 prevailing in China.

Key words: porcine circovirus type 2, ORF2, genotype