中国农业科学 ›› 2008, Vol. 41 ›› Issue (7): 2162-2167 .doi: 10.3864/j.issn.0578-1752.2008.07.039

• 兽医 • 上一篇    下一篇

临床型乳房炎与正常奶牛血浆的差异蛋白质组研究

杨永新,赵兴绪,张 勇,陶金忠   

  1. 甘肃农业大学动物医学院
  • 收稿日期:2007-05-25 修回日期:2007-10-25 出版日期:2008-07-10 发布日期:2008-07-10
  • 通讯作者: 赵兴绪

Comparative proteomics analysis of plasma from healthy dairy cows and clinical mastitic cows

  

  1. 甘肃农业大学动物医学院
  • Received:2007-05-25 Revised:2007-10-25 Online:2008-07-10 Published:2008-07-10

摘要: 【目的】检测和分析临床型乳房炎与正常奶牛血浆中差异表达的蛋白质。【方法】用二维凝胶电泳分离血浆蛋白,凝胶用硝酸银染色和考马斯亮蓝染色,PDQuest7.4软件自动检测分析差异表达蛋白斑点并经高效液相色谱串联离子阱质谱鉴定。【结果】凝胶图谱分析后得到3个差异蛋白斑点,鉴定为2种蛋白质(结合珠蛋白前体和乳腺球蛋白)。结合珠蛋白前体在临床型乳房炎奶牛血浆中表达量增加,可作为乳房炎诊断标记分子;而乳腺球蛋白的表达量下调。【结论】临床型乳房炎与正常奶牛的血浆中蛋白质的表达存在差异,对差异表达蛋白质的研究有利于探索机体的生理病理状态,可为疾病的诊断和治疗提供全面的信息。

关键词: 奶牛, 临床型乳房炎, 血浆, 差异表达蛋白质

Abstract: To investigate the differential proteomic expressions in plasma from healthy dairy cows and clinical mastitic cows, the current research presents the protein changes using two-dimensional gel electrophoresis (2-DE), after the stained with silver nitrate and colloidal coomassie, differential expression proteins were detected by PDQuest 7.4 software and subjected to ion trap mass spectrometer equipped with a Surveyor HPLC system, differential spots of protein were identified. The results showed that 3 protein spots were differentially expressed proteins and identified to be 2 proteins. Overall, haptoglobin precursor was an up-regulated in cows infected with clinical mastitis and could be a mastitis-associated diagnostic marker, while SCGB 2A1 (secretoglobin, family 2A, member 1) was down-regulated protein. There was a significant difference at protein level in plasma from healthy dairy cows and clinical mastitic cows, it suggests that the differential proteome expression analysis of may be useful to clarify the mechanisms involved in the pathophysiology, and to further study of the related proteins and the molecular markers of clinical mastitis.

Key words: dairy cows, clinical mastitis, plasma, proteome