中国农业科学 ›› 2008, Vol. 41 ›› Issue (6): 1838-1844 .doi: 10.3864/j.issn.0578-1752.2008.06.035

• 兽医 • 上一篇    下一篇

肉种鸡MDV和REV人工共感染的动态病理学与抗原定位研究

刁秀国,张 利,成子强,朱 国,王桂花,孟祥凯,高婷婷,崔治中   

  1. 山东农业大学动物科技学院
  • 收稿日期:2007-04-13 修回日期:2007-07-10 出版日期:2008-06-10 发布日期:2008-06-10
  • 通讯作者: 成子强

Dynamic pathology and antigen location study on broiler breeders with coinfection of MDV and REV

Guo ZHU Zi-qiang CHENG Gui-hua WANG Xiang-kai MENG Ting-ting GAO Zhi-zhong CUI   

  1. 山东农业大学动物科技学院
  • Received:2007-04-13 Revised:2007-07-10 Online:2008-06-10 Published:2008-06-10

摘要: 【目的】深入了解MDV与REV人工共感染肉种鸡后疾病的发生、发展状况,为二者临床复杂的混合感染提供确实的鉴别诊断方法和明确的诊断时间。【方法】MDV和REV强毒株人工共感染1日龄肉种鸡,定期剖检,进行病理组织学、细胞凋亡、免疫组化和肿瘤组织的超微结构观察。【结果】肝、胰腺、腺胃、盲肠扁桃体、心肌在感染后1周出现以小淋巴细胞浸润为主的炎症,2~9周时,大部分实质器官出现以幼稚淋巴细胞、大中小淋巴细胞为主的渐进性增生灶,部分增生灶中可见原始网状细胞和马立克氏病细胞;10周后免疫器官实质细胞出现明显的凋亡;免疫组化显示,肝、脾、法氏囊、胸腺等在2周时呈双抗原阳性;电镜下,肝脏肿瘤组织中可见多形态淋巴细胞,核分裂相和细胞凋亡同时存在,同一细胞中可见MDV和REV两种病毒粒子。【结论】MDV与REV共感染对病程起协同和促进作用,发病早,病变明显;免疫酶及荧光方法可用于共感染的早期诊断(2周以前);而后期(4周以后)可通过病理组织学进行鉴别诊断,两种病毒粒子、马立克氏病细胞、原始网状细胞、多形态和幼稚淋巴细胞可作为诊断依据。

关键词: 马立克氏病病毒, 网状内皮组织增殖病病毒, 共感染, 动态病理学, 抗原定位, 肉种鸡

Abstract: 【Objective】To deeply understand the generation and development of coinfection of MDV and REV in broiler breeders,then find the optimal time of differential diagnosis.【Method】We studied pathohistological changes, apoptosis, immunohistochemistry and ultrastructure of tumor issues of broiler breeders inoculated with MDV and REV.【Result】The study showed that proliferation of small lymphocytes were seen in main organs at 1 week old, then immature lymphocytes,all kinds of lymphocytes,primitive reticular cells and Marek’s disease cells were observed after 2 weeks. Cell apoptosis couldn’t be seen untill 10 week in immune system. Immunohistochemistry detection showed that two special cells were observed in main organs at 2 weeks old. Multi-morphology Lymphocytes, MDV and REV, mitotic figures and apoptosis of lymphocytes were observed with transmission electron microscope.【Conclusion】Dynamic pathohistological study showed that pathological changes of coinfection chickens is earlier and manifester than single infection chickens. Coinfection chickens showed that inflammation is main phenomenon in early stage and tumour cells proliferated in late stage. So differential diagnosis can be done by pathohistology in early stage. Marek’s disease cells, primitive reticular cells, immature lymphocytes and two virus can serve as a basis for differential diagnosis. Immunohistochemistry detection is accurate method for late stage.

Key words: Marek’s disease virus, Reticuloendotheliosis virus, coinfection, dynamic pathology, antigen location